Gold nano composite material of high target tumor, and preparation method and application of gold nano composite material

A composite material and gold nanotechnology, applied in the field of medicine, can solve problems such as unsatisfactory treatment of tumors, and achieve the effects of reducing toxic and side effects, high targeting, and reducing dosage

Inactive Publication Date: 2017-07-07
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the deficiencies in the prior art above, the purpose of the present invention is to provide a highly tumor-targeting gold nanocomposite material and its prep

Method used

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  • Gold nano composite material of high target tumor, and preparation method and application of gold nano composite material
  • Gold nano composite material of high target tumor, and preparation method and application of gold nano composite material
  • Gold nano composite material of high target tumor, and preparation method and application of gold nano composite material

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Experimental program
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Embodiment 1

[0052] 1. Synthesis of gold nanoparticles by citric acid reduction method.

[0053] 2. Through the traditional sodium chloride hybridization method, the hybridized double-stranded DNA was obtained by incubating on a constant temperature shaker at 37 degrees. The DNA sequence is shown in Table 1.

[0054] 3. Synthesis of AMD3100 coupled with mercapto PEG: use polyethylene glycol with a molecular weight of 650, dissolve 2 mol of PEG in tetrahydrofuran; dissolve 3 mol of AMD3100 in water, adjust the pH to 7~8 with HCl; mix PEG and AMD3100 was mixed according to the mass ratio of 1:1.5, stirred and reacted at room temperature for 48 hours, and tetrahydrofuran was evaporated to obtain PEG modified with AMD3100.

[0055] 4. At room temperature, put 4×10 -9 mol gold nanometer, 2×10 -5 mol double-stranded DNA and the above steps to obtain PEG 2×10 modified with AMD3100 -5 mol The three are mixed and stirred for 24 hours to obtain the complex of the three.

[0056] 5. Embedding do...

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Abstract

The invention discloses a gold nano composite material of a high target tumor, and a preparation method and application of the gold nano composite material. The gold nano composite material is prepared from doxorubicin, AMD3100, double-chain DNA (deoxyribonucleic acid), PEG (polyethylene glycol) and gold nano particles; the doxorubicin is loaded on the double-chain DNA, and the AMD3100 is modified on the PEG; and the double-chain DNA and the PEG are connected onto the gold nano particles by virtue of sulfydryl. According to the gold nano composite material of the high target tumor, the anticancer drug doxorubicin is used for achieving a chemotherapy effect on tumor cells, and a small molecular inhibitor AMD3100 can be combined with a CXCR4 receptor highly expressed on the surface of the tumor cells in a targeting way. Since the AMD3100 can be combined with and can lower the expression of the CXCR4 and can also inhibit the invasion transfer of the tumor, the combination of the AMD3100 and the CXCR4 can effectively improve an effect for killing the liver cancer, can reduce the consumption of the doxorubicin, and can reduce the toxicity and adverse effect for the normal cells by specifically targeting a liver cancer tissue.

Description

technical field [0001] The invention relates to the field of medicine, and mainly relates to a highly tumor-targeting gold nanocomposite material and its preparation method and application. Background technique [0002] Cancer is one of the diseases with the highest mortality worldwide. Traditional chemotherapy has achieved remarkable results in the clinical treatment of tumors. However, the side effects of anticancer drugs seriously limit their application. In order to improve the treatment of tumor cells by drugs and reduce the side effects of drugs, various nano-drug delivery systems have been developed, such as targeted nano-liposomes, polymer nano-materials, and dendritic polymer carriers. Among them, gold nanoparticles are widely used in nano-drug delivery systems because of their simple preparation methods, easy modification, and low toxicity. However, the current gold nano-targeted drug-loading system often achieves tumor targeting through its own EPR effect or co...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/30A61K47/02A61K47/60A61K31/704A61K31/395A61P35/00
CPCA61K9/5115A61K9/513A61K31/395A61K31/704A61K2300/00
Inventor 张蓉刘亚丽黄景彬李国兵王庆周丽张倩唐勤胡长鹏
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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