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Rubus hirsutus anthocyanin and application thereof

A technology of anthocyanins and fennel, which is applied in the field of medicine, and can solve problems such as the research and report on the hypoglycemic effect of anthocyanins in lentils.

Active Publication Date: 2017-07-07
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the components of anthocyanins in Pennywort have not been revealed clearly, and there are no studies and reports on the hypoglycemic effect of Anthocyanins in Pennywort in vivo

Method used

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  • Rubus hirsutus anthocyanin and application thereof
  • Rubus hirsutus anthocyanin and application thereof
  • Rubus hirsutus anthocyanin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of embodiment 1 pomegranate anthocyanins

[0021] (1) Wash 1000g of fresh pomegranates, add 70% ethanol solution according to the ratio of material to liquid ratio 1:6 (w / v) and mix thoroughly, ultrasonically extract for 90min, vacuum pumping rate after ultrasonic, vacuum at 60°C Rotary evaporation removes ethanol, obtains the crude extract of the anthocyanin of the rotundum;

[0022] (2) Inject the crude extract of the pomegranate anthocyanins into the macroporous resin, rinse the resin with deionized water at a flow rate of 2BV / h, and then use 40%-80% ethanol solution with a flow rate gradient of 1BV / h Elution, collect the eluate with obvious red color, remove ethanol by vacuum rotary evaporation at 60°C, and freeze-dry to obtain the anthocyanin of fennel. According to calculation, 1000g of fresh fennel can be purified by the above steps to obtain 2.81g of flower color of fennel. glycosides.

Embodiment 2

[0023] Example 2 Identification of the Anthocyanin Components of Pompomia

[0024] The anthocyanin of the rotundum in Example 1 is made into the solution of 1mg / mL, and detects with high performance liquid phase, detection method is as follows: mobile phase is A phase (0.1% acetonitrile), B phase (1.5% formic acid aqueous solution), gradient Elution, 5%-60% Phase A elution for 30min, injection volume 10μL, temperature 30℃, flow rate 0.8mL / min, detection wavelength 520nm. At the same time, cyanidin-3-O-glucoside, geranidin-3-O-glucoside and geranidin-3-O-rutinoside standard substances were used for qualitative and quantitative analysis. figure 1 It is the high performance liquid chromatogram of the anthocyanin components of Pompanoderma, peak 1 is cyanidin-3-O-glucoside, peak 2 is geranidin-3-O-glucoside, and peak 3 is geranidin-3-glucoside O-rutinoside, of which geranium-3-O-glucoside content accounts for 89.14% of the total anthocyanin content. The chemical structural formu...

Embodiment 3

[0031] Example 3 The α-glucosidase inhibition experiment of fenugreek anthocyanins

[0032] Prepare a series of concentrations of Punica anthocyanin solution, mix 5 μL α-glucosidase solution (5U / ml, (dissolved in PBS, pH 6.8) and 5 μL PBS solution, add 500 μL PBS solution, and react at 37°C for 20 minutes. Add 10 μL of 10 mM pNPG (p-nitrophenyl-α-D-glucopyranoside) solution, react at 37 °C for 30 min, then add 500 μL of 1mol / L Na 2 CO 3 , the absorbance value is measured at 405nm, according to the formula (1-A sample 405 / A control405 ) to calculate the α-glucosidase half inhibitory concentration IC 50 . figure 2 and image 3 The results showed that the anthocyanins of Pompanoderma can effectively inhibit the activity of α-glucosidase, and its half inhibitory concentration IC 50 It was 0.23 μg / mL, and its activity was significantly stronger than that of the positive drug acarbose (33 μg / mL). Therefore, the anthocyanins of Pompomia can effectively inhibit the activity o...

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Abstract

The invention discloses rubus hirsutus anthocyanin and application thereof. The rubus hirsutus anthocyanin mainly comprises cyanidin-3-O-glucoside, pelargonine 3-O-glucoside and pelargonine 3-O-rutinoside, wherein the pelargonine 3-O-glucoside is main anthocyanin. An animal experiment proves that the rubus hirsutus anthocyanin can significantly reduce fasting blood glucose of db / db diabetic mice and shows excellent hypoglycemic activity in vivo. An in-vitro experiment proves that the rubus hirsutus anthocyanin can play a hypoglycemic role by inhibiting the alpha-glucosidase activity and promoting the glucose consumption of cells. Therefore, the rubus hirsutus anthocyanin can be taken as hypoglycemic functional food or a hypoglycemic drug for the prevention and the treatment of glycometabolism disorder-related diseases. The invention provides a new idea for comprehensive development and utilization of rubus hirsutus resources in China.

Description

technical field [0001] The present invention relates to the field of medicine, in particular to a pombe anthocyanin and its application, especially the application of the natural source pompon anthocyanin in the preparation of hypoglycemic functional food, health care product or hypoglycemic drug. Background technique [0002] Diabetes is one of the most important non-communicable diseases currently threatening human health. The development of diabetes is often accompanied by the outbreak of other metabolic syndromes, such as hyperlipidemia, kidney failure and inflammation, which bring great physical and mental pain to human beings. There are two types of diabetes: type 1 diabetes (insulin dependent) and type 2 diabetes (non-insulin dependent). According to the statistics of the International Diabetes Federation (IDF), the number of diabetic patients in the world reached 370 million in 2011, and the number of type 2 diabetic patients accounted for 90% of the total number of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048C07H17/065C07H1/08A23L33/105
CPCA23V2002/00A61K31/7048A61K36/73C07H1/08C07H17/065A23V2200/328A23V2250/21A23V2300/14
Inventor 陈卫徐阳鲍涛苏鸿铭李玉婷
Owner ZHEJIANG UNIV
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