Construction method of molecular marker fingerprint of radix rehmanniae and identification method of frequent cultivated varieties thereof

Abstract

The invention discloses a construction method of a molecular marker fingerprint of radix rehmanniae and an identification method of frequent cultivated varieties thereof. The method comprises the following steps of: collecting and pre-treating a fresh radix rehmanniae sample; extracting and purifying a fresh radix rehmanniae sample genomic DNA; performing SCoT-PCR amplification; performing agarose gel electrophoresis; constructing SCoT molecular marker fingerprints of test samples; and identifying seven frequent radix rehmanniae cultivated varieties by means of the constructed SCoT molecular fingerprints and the like. The method disclosed by the invention can save time and cost, is convenient to operate and fast and accurate; an accurate and reliable identification fingerprint can be obtained by SCoT-PCR amplification and agarose gel electrophoresis; the fingerprints are constructed by six screened SCoT primers and seven frequent radix rehmanniae cultivated varieties: sweet potato king, Kangyu 831, Beijing #3, Beijing #2, Jinzhuangyuan, Jinjiu and Wwen 85-5 are differentiated.

Description

technical field [0001] The invention belongs to the technical field of identification methods for common cultivars of Rehmannia glutinosa, and in particular relates to the construction of molecular marker fingerprints of rehmannia rehmanniae and an identification method for common cultivars thereof. Background technique [0002] Rehmannia glutinosa (Rehmannia glutinosa) is a perennial herbaceous plant of the genus Rehmannia in the family Scrophulariaceae. It includes six species of Rehmannia glutinosa, Rehmannia lobata, Rehmannia glutinosa, Rehmannia glutinosa, Rehmannia glutinosa and Rehmannia rehmannia, distributed in China, Japan, Korea and other countries. In my country, Rehmannia glutinosa is mainly distributed in Henan, Shanxi, Liaoning, Shandong, Shaanxi and Anhui. As a medicine and food material, rehmannia root has important economic value. Therefore, Rehmannia glutinosa is favored by people and widely studied. [0003] The target start codon polymorphism molecular...

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Problems solved by technology

However, SCoT markers have not been reported on Rehmannia glutinosa

[0004] In addition, due to the long-term vegetative propagation of Rehmannia glutinosa and the introduction and exchange of varieties between intervals, most varieties degenerate after years of planting, with serious declines in quality and yield, and uneven external forms

However, the current research on Rehmannia glutinosa mainly includes breeding, chemical regulation, and pest control, and there are relatively few studies on the genetic diversity and genetic relationship of Rehmannia glutinosa from a molecular perspective.

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