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A kind of multifunctional electrochemical sensor and preparation method thereof

A sensor and electrochemical technology, applied in the field of electrochemistry, can solve problems such as inability to have, and achieve the effects of easy control, improved sterilization effect, and low cost

Active Publication Date: 2019-05-28
HAINAN MICROKRYPTON BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current electrochemical sensors can only simply detect bacteria, and cannot have the multi-functional functions of simultaneous removal, detection, and sterilization

Method used

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  • A kind of multifunctional electrochemical sensor and preparation method thereof
  • A kind of multifunctional electrochemical sensor and preparation method thereof
  • A kind of multifunctional electrochemical sensor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation method of vancomycin-functionalized AgNPs@ZnO / FTO multifunctional sensor:

[0045] (1) Preparation of ZnO / FTO: 50mM Zn(NO 3 ) 2 ·6H 2 O was added into ultrapure water, magnetically stirred until it was completely dissolved, and named solution A; at the same time, 50mM hexamethylenetetramine was added into ultrapure water and magnetically stirred to complete solution, named solution B; then solution A and solution B Mix equal volumes. Afterwards, immerse the cleaned FTO wrapped in aluminum sheets into the above mixed solution, place the conductive surface vertically downward, and put it in a water bath at 90°C for 3 hours; then cool the solution to room temperature, take out the FTO, rinse and heat at 40°C After drying for 6h, ZnO-modified FTO with a columnar structure can be obtained (such as figure 1 A);

[0046] The FTO after the cleaning is that the FTO is cleaned successively with acetone, ethanol / water NaOH mixed solution, and ultrapure water for 1...

Embodiment 2

[0051] Example 2: The multifunctional sensor obtained above is applied in water bodies, and its detection performance on harmful microorganisms Staphylococcus aureus.

[0052] Take Staphylococcus aureus (500~10 6 ) evaluation of vancomycin-functionalized AgNPs@ZnO / FTO multifunctional sensor:

[0053] First prepare the bacterial suspension, inoculate the stock solution of Staphylococcus aureus into the sterilized LB liquid medium, then place it in an air constant temperature shaker at 37°C and 150rpm, and cultivate it overnight. The cultured bacterial suspension was centrifuged and suspended in 0.01mol / L PBS (pH=7.4) buffer to obtain a concentration of 1×10 8 cfu / mL Staphylococcus aureus suspension, ready to use

[0054] In the detection experiment, dilute the above-mentioned bacterial suspension to 100-10 with sterile 0.01mol / L PBS (pH=7.4) buffer solution. 6 cfu / mL concentration gradient, and then immerse the multi-functional sensor prepared by the present invention in bac...

Embodiment 3

[0057] The vancomycin-functionalized Ag@ZnO / FTO multifunctional sensor obtained above can kill and isolate harmful microorganisms Staphylococcus aureus in water.

[0058] Sterilization experiment:

[0059] Get staphylococcus aureus stock solution and dilute to 10 with sterilized 0.01mol / L PBS (pH=7.4) buffer solution 6 cfu / mL. Place the diluted bacterial solution in a 5mL reactor, add the multifunctional sensor prepared in Example 1 and immerse it in the bacterial solution, take 1.0mL of the reaction solution after 12 hours of reaction, buffer with 0.01mol / L PBS (pH=7.4) According to the serial dilution method, several gradients (10, 100, 100 times) were successively diluted, and then 100 μL were taken from the solutions of different dilution times on the prepared LB solid medium, and the bacterial solution was evenly spread on the LB culture medium. basically. Invert the LB medium, put it into an electric thermostat incubator and incubate at 37°C for 24 hours, count the nu...

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Abstract

The invention relates to the field of electrochemistry, and concretely relates to a multifunctional electrochemical sensor for simultaneously realizing capture, sterilization and detection, and a making method thereof. The sensor is ZnO nanorods loaded with AgNPs and vancomycin, wherein a mass ratio of the AgNPs to the vancomycin is 1:1 to 1: 6. The preparation method has the advantages of simple process, easiness in control and low cost, and allows the multi-functional electrochemical sensor for simultaneously realizing capture, sterilization and detection to be produced. The synergistic effect of the vancomycin and nano-silver is used to highly-efficiently kill the harmful microorganisms in water and separate and detect pathogens in the water, so the sensor has very good application prospect in the field of water purification.

Description

technical field [0001] The invention belongs to the field of electrochemistry, and in particular relates to a multifunctional electrochemical sensor capable of capturing, sterilizing and detecting at the same time and a preparation method thereof. Background technique [0002] Every year, many people die from bacterial water pollution, such as "super bacteria" pollution and so on. If bacterial contamination can be diagnosed quickly and the bacteria killed quickly, the mortality rate of bacterial infection will be greatly reduced [1]. [0003] Electrochemical biosensor is an analytical method based on solid electrodes and biologically sensitive molecules. Because electrochemical sensors have the advantages of high efficiency, simplicity, easy operation and low cost, electrochemical sensors have been widely used in the analysis of DNA [2] and viruses [3]. Among many bacterial detection technologies, electrochemical biosensors have attracted widespread attention. For example,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26B82Y5/00B82Y15/00B82Y30/00B82Y40/00C02F1/461
CPCB82Y5/00B82Y15/00B82Y30/00B82Y40/00C02F1/46109C02F2303/04G01N27/26
Inventor 杨治庆张盾王毅
Owner HAINAN MICROKRYPTON BIOTECHNOLOGY CO LTD
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