Lung cancer monitoring kit and use method thereof

Abstract

The invention provides a lung cancer monitoring kit and a use method thereof. The kit includes: a reagent A, which is prepared by adding 5% of SDS to a 10 mM ammonium bicarbonate solution; a reagent B, which is prepared by adding not less than 2.2 unit / [mu]L of exoglycosidase to NP40 being 3.3% in concentration; a reagent C, which is a mixture liquid composed of, in equal volume, a solution of 20 mM of APTS dissolved in 1.2 M of citric acid and a solution of 1M of an organic reducing agent dissolved in DMSO; a reagent D, which includes 0.25 [mu]L of NH4Ac with concentration being 100 mM and pH value being 5, 0.2 [mu]L of sialidase, and 2.55 [mu]L of H2O2. The kit can evaluate lung cancer patients with detection on a fingerprint spectrum of an oligosaccharide chain in blood glycoprotein as a diagnosis index, thereby diagnosing the tumor stage of the lung cancer. The kit allows numerous lung cancer patients to take regular and non-invasive detection, and helps a medicinal worker to detect the lung cancer and monitor progression of the lung cancer timely.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a lung cancer monitoring kit and a use method thereof. Background technique [0002] Worldwide, lung cancer is the leading cause of cancer death in both men and women. In 2005, it was estimated that there were about 500,000 new cases of lung cancer in China (about 330,000 cases in men and about 170,000 cases in women). There are two main reasons for the high mortality rate of lung cancer: one is the lack of effective early diagnosis methods for lung cancer, and more than 70% of the patients are diagnosed at an advanced stage; the other is that advanced lung cancer (especially non-small cell lung cancer, NSCLC) is sensitive to chemotherapy The overall effective rate of various existing chemotherapy regimens for NSCLC is only about 30%. [0003] Lung cancer can be divided into two types according to anatomical classification: central lung cancer and peripheral lun...

AI Technical Summary

Problems solved by technology

There are two main reasons for the high mortality rate of lung cancer: one is the lack of effective early diagnosis methods for lung cancer, and more than 70% of the patients are diagnosed at an advanced stage; the other is that advanced lung cancer (especially non-small cell lung cancer, NSCLC) is sensitive to chemotherapy The overall effective rate of various existing chemotherapy regimens for NSCLC is only about 30%.

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