Esophageal cancer detection reagent and application thereof in esophageal cancer detection

Abstract

The invention provides an esophageal cancer detection reagent and a preparation method thereof, and the detection reagent is prepared by mixing the following reagents: a reagent A which is prepared by adding 0.5-5% by mass of SDS into an ammonium bicarbonate solution with a concentration of 10 mM; a reagent B which is a mixed solution prepared by mixing 0.01-10 U / 10 [mu]L of glycosamine acylase and 0.01-10 U / 10 [mu]L of sialidase and has a pH value of 4-9; a reagent C which is prepared by dissolving 8-aminopyrene-1,3,6-trisulfonic acid in DMSO (dimethylsulfoxide) and has a concentration of 0.01 mM to 1M; and a reagent D which is a stop solution. According to the invention, a glycome map in serum is determined by a detection reagent, a peak value is quantified for statistical analysis, and the establishment method of the esophageal cancer serum glycome map model is provided for detecting esophageal cancer.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a detection method for esophageal cancer, in particular to a detection method for esophageal cancer based on the specific fingerprint of serum glycoprotein oligosaccharide chain detection (G-Test). Background technique [0002] Esophageal cancer (Esophageal Carcinoma) is a common malignant tumor in humans, accounting for more than 90% of esophageal tumors, ranking second only to gastric cancer in the retrospective survey of all malignant tumor deaths. It is estimated that about 200,000 people die of esophageal cancer every year in the world, and it is one of the most common malignant tumors. Early esophageal cancer has no obvious clinical symptoms. It is mostly caused by local lesions stimulating abnormal esophageal motility or spasm, or local inflammation, erosion, superficial ulcers, and tumor infiltration. It often occurs repeatedly, and the intermittent period can be asympto...

AI Technical Summary

Problems solved by technology

2. There are no highly sensitive and specific markers for tumor markers

However, the mass spectrometry instrument is precise, the operation of the equipment is complicated, and the mass spectrometer is expensive, so it is not suitable for popularization and use in clinical practice; (3) capillary electrophoresis (CE): capillary electrophoresis is low in cost, high in column efficiency, high in sensitivity, fast in speed, and easy to inject. The amount is small and the operation is simple, but the repeatability is not high and the stability is not as good as HPLC

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