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use 15 N labeling method for studying nitrogen transfer in phytoplankton and aquatic plants

A technology for aquatic plants and planktonic algae, which is applied in the fields of testing plants/trees, sample preparation for testing, instruments, etc., can solve problems such as lack of unified understanding, and achieve the effect of avoiding overload operation, accurate detection, and convenient migration.

Active Publication Date: 2020-08-04
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But about phytoplankton 15 The research on N labeling is still insufficient, for the growth of phytoplankton in the aquatic environment 15 The abundance of N and the optimum of phytoplankton 15 Lack of unified understanding of N label quantity

Method used

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  • use <sup>15</sup> N labeling method for studying nitrogen transfer in phytoplankton and aquatic plants
  • use <sup>15</sup> N labeling method for studying nitrogen transfer in phytoplankton and aquatic plants
  • use <sup>15</sup> N labeling method for studying nitrogen transfer in phytoplankton and aquatic plants

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Embodiment 1

[0022] Embodiment 1: A kind of method utilizing 15N labeling to study nitrogen migration in Microcystis aeruginosa and Eichhornia chinensis;

[0023] 1) Take Microcystis aeruginosa ( Microcystis aeruginosa FACHB 905) as an example, a 500 mL Erlenmeyer flask was used to culture in a light incubator, the temperature was 25°C±1°C, and the light intensity was 30 µmol photons m -2 ·s -1 , the photoperiod is 12:12, using BG 11 The culture medium was cultured statically, with Na 15 NO 3 In the culture medium 5 N abundance was adjusted to 5%, and labeled algal cells were collected after 14 days.

[0024] for the determination of algae 15 N-abundant algal cells were collected by GF / C membrane suction filtration, 10ml of algae liquid was filtered onto the GF / C membrane with a vacuum pump, and the membrane was placed in a freeze dryer and freeze-dried to constant weight. Cut the dried filter membrane into 1mm particles with scissors, save the sample, and measure its concentratio...

Embodiment 2

[0029] Embodiment 2: a kind of utilization 15 The method of N labeling to study the transfer of nitrogen in Microcystis aeruginosa and water plantain;

[0030] Experimental method: 1) Put Microcystis aeruginosa into a 500 mL Erlenmeyer flask and culture it in a light incubator at a temperature of 25°C±1°C and a light intensity of 30 µmol photons m -2 ·s -1 , the photoperiod is 12:12, using BG 11 The culture medium was cultured statically, with Na 15 NO 3 In the culture medium 5 N abundance was adjusted to 5%, and labeled algal cells were collected after 16 days.

[0031] for the determination of algae 15 N-abundant algal cells are collected by GF / C membrane suction filtration method, take 10ml of algae liquid and filter it onto the GF / C membrane with a vacuum pump, and place the membrane in a freeze dryer to freeze-dry to constant weight; use scissors to filter the dried Cut the film into 1.5mm particles, save the sample, and measure its density with a stable isotope ma...

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Abstract

The invention discloses a method for researching nitrogen migration of floating algae and aquatic plants by using 15N marking. The method comprises the steps of culturing floating algae cells in a liquid culture medium containing Na15NO3 for 10-16d, collecting algae cells through a GF / C membrane suction filter method and measuring the abundance of 15N in the algae cells through an isotope mass spectrometer; collecting the algae cells marked by the 15N, co-culturing the algae cells and the aquatic plants for 18-30d, wherein the abundance of the 15N in the marked algae cells is 3.8-4.8%; and measuring the abundance of the 15N in the aquatic plants to be 2.7-3.9% through the isotope mass spectrometer to obtain the g nitrogen migration and conversion efficiency between the floating algae and the aquatic plants. The experiment condition and method are limited, the condition that the subsequently measured migration results are controlled within a specified threshold is ensured, overload operation of a detecting instrument is avoided, the detection is accurate and the migration effect of the floating algae and the aquatic plants is convenient to calculate.

Description

technical field [0001] The invention relates to the field of research on eutrophication of water bodies, in particular to a 15 N labeling method for studying nitrogen transport in phytoplankton and aquatic plants. Background technique [0002] Eutrophication is the main problem facing lakes in China. Frequent outbreaks of cyanobacterial blooms are an important natural sign of the gradual increase in eutrophication, and it is also one of the main environmental problems in China. Nitrogen is one of the key nutrient elements that cause eutrophication of water bodies, so reducing nitrogen is undoubtedly one of the prerequisites for the treatment of eutrophic water bodies. For algae-type eutrophic water bodies, among many technical methods for removing endogenous nitrogen, ecological interception and aquatic phytoremediation technologies are effective measures to remove cyanobacteria and restore the self-purification ability of water bodies, and have good research and applicatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62G01N1/28G01N1/34
CPCG01N1/28G01N1/34G01N27/62G01N33/0098
Inventor 秦红杰刘旻慧张志勇闻学政王岩张迎颖
Owner JIANGSU ACAD OF AGRI SCI