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ATP nucleic acid aptamer, method and kit for chromogenic detection of ATP using nano-gold

A nucleic acid aptamer and nano-gold technology, which is applied in color/spectral characteristic measurement, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of cumbersome operation steps, expensive instruments, time-consuming and labor-consuming, and achieve high sensitivity , easy operation and low cost

Active Publication Date: 2020-12-04
SHENZHEN KIVITA INNOVATIVE DRUG INST
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

These methods have good sensitivity and specificity, but require expensive instruments and cumbersome procedures, and are time-consuming and labor-intensive

Method used

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  • ATP nucleic acid aptamer, method and kit for chromogenic detection of ATP using nano-gold
  • ATP nucleic acid aptamer, method and kit for chromogenic detection of ATP using nano-gold
  • ATP nucleic acid aptamer, method and kit for chromogenic detection of ATP using nano-gold

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Embodiment 1

[0040] Kit composition

[0041] a) Detection solution: 20mM Tris-HCl buffer (100mM NaCl, 5mM MgCl 2 , pH 7.4) (contains a DNA probe with the sequence shown in SEQ ID NO: 1: 1 μM)

[0042] b) NaCl solution (1.6M)

[0043] c) Nano gold sodium citrate buffer solution (14nm, concentration is 1nM)

[0044] Solution to be tested containing ATP

[0045] ATP aqueous solution, wherein the ATP concentration is 10 μM.

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Abstract

The invention provides an ATP aptamer, and a method and a kit which are used for detecting ATP via nanogold color development. The ATP aptamer possesses a base sequence represented by SEQ ID NO:1. The method comprises following steps: 1, an ATP-containing solution to be tested is mixed with a detection solution containing the ATP aptamer; 2, a mixed solution obtained in step 1 is mixed with a nanogold solution, and a salt solution is added for uniform mixing; 3, an obtained mixed liquid obtained in step 2 is subjected to instrument detection or observation. According to the method, detection of ATP is realized, no chemical modification is needed, no complex reaction process is needed, no expensive equipment is used, detection can be completed even via naked-eye observation, operation is simple and convenient, cost is low, detection time is short, the kit is convenient to use, sensitivity is high, and specificity is high.

Description

technical field [0001] The invention relates to the technical field of biological detection, and more specifically, relates to an ATP nucleic acid aptamer, a method and a kit for detecting ATP by color development of nano gold. Background technique [0002] ATP, as an essential universal energy source in the synthesis of cells for the survival and reproduction of all organisms, plays a very important role in the metabolic process of the body. Studies have shown that ATP can be used as an indicator of cell survival and cell damage. The abnormal concentration of ATP in cells is closely related to many diseases, such as tumors, cardiovascular diseases, Parkinson's syndrome and so on. Therefore, rapid and accurate detection of ATP has great significance in clinical application. [0003] The existing ATP detection methods mainly include HPLC method, luciferin / luciferase method and electrophoresis method. These methods have good sensitivity and specificity, but require expensiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/115G01N21/33
CPCC12N15/115G01N21/33
Inventor 张晶珠樊婷婷毛瑜李露露
Owner SHENZHEN KIVITA INNOVATIVE DRUG INST
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