Method for distinguishing gymnodiptychus dybowskii distributed in southern and northern Xinjiang regions by utilizing ISSR molecular marker technology

Abstract

The invention discloses a method for distinguishing gymnodiptychus dybowskii distributed in southern and northern Xinjiang regions by utilizing an ISSR molecular marker technology. The method comprises the following steps that 1, a gymnodiptychus dybowskii genome DNA is extracted; 2, the gymnodiptychus dybowskii genome DNA is amplified by utilizing ISSR-PCR reaction; 3, a gymnodiptychus dybowskii DNA electrophoresis mode chart is established; 4, the gymnodiptychus dybowskii distributed in the southern and northern Xinjiang regions is distinguished. The gymnodiptychus dybowskii is distinguished by utilizing the ISSR molecular marker technology, geographical populations distributed in different water systems in the southern and northern Xinjiang regions are distinguished according to genetic characteristics, a reliable basis is provided for species distinguishing, and a technical guarantee is also provided for enhancement and releasing of a parent source and fry quality.

Description

technical field [0001] The invention relates to a method for using ISSR molecular marker technology to identify Xinjiang Pyramidus undatus distributed in northern and southern Xinjiang, and belongs to the field of biotechnology. Background technique [0002] Xinjiang naked heavy lip fish ( Gymnodiptychus dybowskii ), commonly known as naked cucumber fish, belongs to Cypriniformes (Cypriniformes), Cyprinidae (Cyprinidae), Schizothoracinae (Schizothoracinae), naked heavy lip fish ( Gymnodiptychus ). It is mainly distributed in the Yili River Basin in my country, the waters of the Junggar Basin on the northern slope of the Tianshan Mountains, the Kaidu River in southern Xinjiang, and the Syr Darya River in Central Asia, the upper reaches of the tributaries of Lake Balkhash, and Lake Issyk-Kul. As a representative indigenous species and an ecologically sensitive species in the Yili River system, the wild population resources of the Xinjiang carp have been seriously damaged du...

AI Technical Summary

Problems solved by technology

However, the genetic management of released parents has not been effectively carried out, and there is a phenomenon that the source of released seedling parents is relatively chaotic

Existing studies have confirmed that there are huge differences in the genetic structure of the populations of the Ili River Basin on the northern slope of the Tianshan Mountains and the Kaidu River Basin in southern Xinjiang. Blind release without effective identification of the source of the parents will seriously affect the genetic diversity. sex, causing genetic pollution among species and population degradation, which is not conducive to the effective use of natural resources

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