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Method for extracting senplus from diutan gum fermenting liquid

A technology of diyou gum and fermentation liquid, which is applied in the field of extraction of fermentation products to achieve the effects of mild conditions, improved quality performance and economic value, and fast enzymolysis reaction speed

Active Publication Date: 2017-07-25
张星昊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And since "Food Additive Xanthan Gum" (GB1886.41-2015) has no standardized detection method for its purity, those skilled in the art generally refer to the gellan gum in "Food Additive Gellan Gum" (GB25535-2010) Content detection method to detect the purity of diyou gum

Method used

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  • Method for extracting senplus from diutan gum fermenting liquid
  • Method for extracting senplus from diutan gum fermenting liquid

Examples

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Embodiment 1

[0054] A method for extracting diyout gum from diyout gum fermentation broth, which is to extract diyout gum from the fermented broth after centrifugation and extraction; comprising the following process steps:

[0055] A. Fermentation broth pretreatment

[0056] A1. Add lysozyme with an enzyme activity of 100,000 u / g to the fermented broth containing diyout gum at pH=6-8, the concentration of lysozyme in the fermented broth is 100ppm, stir well and maintain the reaction for 5 hours;

[0057] A2, the fermented liquid after the reaction in step A1 is warmed up to 130 DEG C and maintained for 8 minutes and then lowered to 60 DEG C, adding acid protease with an enzyme activity of 50000u / g, the concentration of acid protease in the fermented liquid is 200ppm, at pH=3- 4. Under the condition of temperature of 50°C, react for 4 hours to make the pretreated fermentation broth;

[0058] B. Primary extraction: According to the ratio of fermented liquid after pretreatment: ethanol solu...

Embodiment 2

[0068] The difference between this embodiment and embodiment 1 is:

[0069] A method for extracting diyout gum from diyout gum fermentation broth, which is to extract diyout gum from the fermented broth after centrifugation and extraction; comprising the following process steps:

[0070] A. Fermentation broth pretreatment

[0071] A1. Add lysozyme with an enzyme activity of 100,000 u / g to the fermented broth containing diyout gum at pH=6-8, the concentration of lysozyme in the fermented broth is 200ppm, stir well and maintain the reaction for 3 hours;

[0072] A2. Raise the temperature of the fermented broth after the reaction in step A1 to 140°C for 5 minutes and then drop to 70°C, add acid protease with enzyme activity of 50000u / g, the concentration of acidic protease in the fermented broth is 400ppm, at pH=3- 4. Under the condition of 60°C, react for 2 hours to make pretreated fermentation liquid;

[0073] B. Primary extraction: According to the ratio of fermented liquid ...

Embodiment 3

[0080] The difference between this embodiment and embodiment 1 is:

[0081] A method for extracting diyout gum from diyout gum fermentation broth, which is to extract diyout gum from the fermented broth after centrifugation and extraction; comprising the following process steps:

[0082] A. Fermentation broth pretreatment

[0083] A1. Add lysozyme with an enzyme activity of 100,000 u / g to the fermented broth containing diyout gum at pH=6-8, the concentration of lysozyme in the fermented broth is 150ppm, stir well and maintain the reaction for 4 hours;

[0084] A2. Raise the temperature of the fermented broth after the reaction in step A1 to 135°C for 7 minutes and then drop to 65°C, add acid protease with enzyme activity of 50000u / g, the concentration of acidic protease in the fermented broth is 300ppm, at pH=3- 4. Under the condition of temperature of 55°C, react for 3 hours to make pretreated fermentation liquid;

[0085] B. Primary extraction: According to the ratio of fe...

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Abstract

The invention belongs to the extracting of fermenting products, in particular to a method for extracting diutan gum from a diutan gum fermenting liquid. The method comprises the following processing steps of pretreatment of fermenting liquid, primary extracting, primary centrifuging, secondary extracting, three times of diluting, and the like. The method has the advantages that the technical problems of low purity and viscosity of a diutan gum product prepared by the existing diutan gum post-extracting technology and the like are solved; compared with the existing product, the technology cycle is short, the control easy, the production cost is low, and the purity and viscosity of product are better.

Description

technical field [0001] The invention belongs to the extraction of fermentation products, in particular to a method for extracting diyou gum from diyou gum fermentation liquid. Background technique [0002] Diyou gum is a new type of microbial polysaccharide following xanthan gum, gellan gum, and Wenlun gum. It is a water-soluble biopolymer produced by microbial fermentation. It has excellent thickening properties, pseudoplasticity, high temperature resistance, and High salt and wide range of pH adaptability and other excellent characteristics, can be widely used in petroleum, building materials and other industries, with broad application prospects. [0003] The existing technology of using microbial fermentation to produce diyou gum includes two parts: fermentation and post-extraction. The applicant disclosed a sphingomonas and a method for preparing Dingyou gum fermentation broth by using the patent number 201410541800.4. The main technical idea is to select sphingomonas ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 张星昊
Owner 张星昊
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