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A gold nanorod/polyethylene glycol/carbon dot nano-hybrid imaging contrast material and its preparation method and application

A technology of polyethylene glycol and gold nanorods, applied in general/multifunctional contrast agents, echo/ultrasonic imaging agents, pharmaceutical formulations, etc. Low fluorescence efficiency of nanorods and other issues, to achieve the effect of improving biocompatibility, good stability, and good water solubility

Active Publication Date: 2020-04-10
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As excellent fluorescent contrast agents, organic dyes and semiconductor quantum dots can be applied in the field of FLIM imaging, but there are still some defects: certain cytotoxicity and photobleaching phenomenon (Are quantum dots toxic? Exploring the discrepancy between cell culture and animal studies.Acc.Chem.Res.,2013,46,662-671)
However, gold nanorods suffer from low fluorescence efficiency and cannot be directly used in FLIM imaging systems.
[0005] Each mode has its own shortcomings. For example, FLIM imaging has the problem of insufficient imaging depth caused by the fluorescence signal being reflected and scattered by the tissue, and DR imaging has the problem of poor accuracy due to its large imaging range.
A single imaging mode can no longer fully meet the requirements of major disease diagnosis

Method used

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  • A gold nanorod/polyethylene glycol/carbon dot nano-hybrid imaging contrast material and its preparation method and application
  • A gold nanorod/polyethylene glycol/carbon dot nano-hybrid imaging contrast material and its preparation method and application
  • A gold nanorod/polyethylene glycol/carbon dot nano-hybrid imaging contrast material and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0050] The preparation of a nano-hybrid imaging contrast material of gold nanorods / polyethylene glycol / carbon dots comprises the following steps:

[0051] 1) Preparation of gold nanorods

[0052] Gold nanorods were prepared by seed-mediated growth method (Preparation and growth mechanism of gold nanorods (NRs) using seed-mediated growth method. Chem. Mater. 2003, 15, 1957-1962). The prepared gold nanorods have a diameter of about 15nm, a length of about 45nm, an aspect ratio of 3, and an ultraviolet absorption peak of 600nm. figure 1 A transmission electron microscope image of the gold nanorod is shown.

[0053] 2) Preparation of gold nanorod / polyethylene glycol composite

[0054] Take 10 mL of the mixture containing gold nanorods prepared in 1) and put it in a centrifuge tube, then add 79.2 μL of mPEG-SH with a concentration of 10 mmol / L and 0.8 μL of HS-PEG-COOH with a concentration of 10 mmol / L ( The ratio of mPEG-SH and HS-PEG-COOH is 99:1), wherein the molecular weight...

Embodiment 2

[0063] The preparation of a nano-hybrid imaging contrast material of gold nanorods / polyethylene glycol / carbon dots comprises the following steps:

[0064] 1) Preparation of gold nanorods

[0065] Gold nanorods were prepared by seed-mediated growth method (Preparation and growth mechanism of gold nanorods (NRs) using seed-mediated growth method. Chem. Mater. 2003, 15, 1957-1962). The obtained gold nanorod has a diameter of 10 nm, a length of 100 nm, an aspect ratio of 10, and an ultraviolet absorption peak at 950 nm.

[0066] 2) Preparation of gold nanorod / polyethylene glycol composite

[0067] Take 10 mL of the mixture containing gold nanorods prepared in 1) into a centrifuge tube, and then add 36 μL of mPEG-SH with a concentration of 10 mmol / L and 36 μL of HS-PEG-NH with a concentration of 10 mmol / L 2 (mPEG-SH and HS-PEG-NH 2 The ratio is 1:1), and the molecular weights of mPEG-SH and HS-PEG-COOH used are both 10000. After stirring at room temperature for 4 h, centrifugin...

Embodiment 3

[0075] The preparation of a nano-hybrid imaging contrast material of gold nanorods / polyethylene glycol / carbon dots comprises the following steps:

[0076] 1) Preparation of gold nanorods

[0077]Gold nanorods were prepared by seed-mediated growth method (Preparation and growth mechanism of gold nanorods (NRs) using seed-mediated growth method. Chem. Mater. 2003, 15, 1957-1962). The obtained gold nanorods have a diameter of about 15 nm and a length of about 45 nm. The aspect ratio is 3, and the ultraviolet absorption peak is 600nm.

[0078] 2) Preparation of gold nanorod / polyethylene glycol composite

[0079] Take 10 mL of the mixture containing gold nanorods prepared in 1) into a centrifuge tube, then add 72 μL of mPEG-SH with a concentration of 10 mmol / L and 8 μL of HS-PEG-COOH with a concentration of 10 mmol / L (mPEG- The ratio of SH and HS-PEG-COOH is 9:1), and the molecular weights of mPEG-SH and HS-PEG-COOH used are both 1000. After stirring at room temperature for 4 h...

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Abstract

The invention discloses a gold nano-rod / polyethylene glycol / carbon dot nano-hybrid imaging contrast material which comprises polyethylene glycol, a gold nano-rod and a carbon dot. The gold nano-rod is connected to one end of the polyethylene glycol, the carbon dot is connected to the other end of the polyethylene glycol, the gold nano-rod is connected with the polyethylene glycol through a gold-sulfur coordination bond, and the carbon dot is connected with the polyethylene glycol through an amido bond. A preparation method of the gold nano-rod / polyethylene glycol / carbon dot nano-hybrid imaging contrast material is simple and controllable, and the unique optical property of the gold nano-rod is kept while water solubility of the gold nano-rod is enhanced. Besides, the material has the surface plasma resonance property of the gold nano-rod and the fluorescent property of the carbon dot. Diffuse reflection imaging can be realized by the aid of excellent optical reflection and scattering properties of the gold nano-rod, and fluorescent lifetime imaging is realized by the aid of the carbon dot, so that multi-modal imaging is realized.

Description

technical field [0001] The invention relates to the technical field of biological imaging. More specifically, it relates to a gold nanorod / polyethylene glycol / carbon dot nano-hybrid imaging contrast material and its preparation method and application. Background technique [0002] With the improvement of disease diagnosis requirements, people put forward higher requirements for the resolution, sensitivity, imaging depth and targeting effect of imaging technology. [0003] Fluorescence lifetime (FLT) imaging microscopy (FLIM) is a spectroscopic technique with high application value in biology and biomedicine, and can be used for quantitative analysis and measurement of the microenvironment of cells. Unlike conventional imaging techniques based on fluorescence intensity, FLIM can obtain better images. Because the fluorescence lifetime is a characteristic of the molecule itself, it is related to the microenvironment of the fluorescent molecule, but has nothing to do with the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K49/22
CPCA61K49/0002A61K49/0054A61K49/0065A61K49/221
Inventor 周树云凌果胡秀杰孙兴明谢政
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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