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Application of reagent for detecting PITX1 (paired-like homeodomain 1) expression in preparation of gastric cancer prognosis evaluation kit

A prognostic evaluation and expression technology, which is applied in the field of biological and medical diagnosis, can solve problems such as differences in diagnosis and treatment, poor prognosis, and differences in the efficacy of chemotherapy drugs for patients. It achieves simple introduction, high feasibility, and easy prediction and evaluation Effect

Active Publication Date: 2017-07-28
SOUTHEAST UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Gastric cancer is a disease that seriously endangers the health of our people. So far, there is no better targeted drug, resulting in poor treatment effect and poor prognosis for patients.
In addition, gastric cancer is a highly heterogeneous disease, and the etiology of different patients is different, resulting in differences in diagnosis and treatment
In particular, different genetic bases may cause differences in the efficacy of chemotherapy drugs in patients
At present, there is no technical means at home and abroad that can effectively judge the prognosis of the differences in the efficacy of chemotherapy drugs.

Method used

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  • Application of reagent for detecting PITX1 (paired-like homeodomain 1) expression in preparation of gastric cancer prognosis evaluation kit

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1 Immunohistochemical detection method of PITX1 expression

[0020] 1. Surgically resected gastric cancer specimens, fixed in neutral formalin, cut into tissue blocks with a thickness of 2-3 mm, embedded in paraffin, and sectioned with a thickness of 3-4 mm;

[0021] 2. Baking slices: 65 ℃, 1~2 hours;

[0022] 3. Dewax the slices to water: first place the slices in xylene solution I for 15 minutes, take them out and place them in xylene solution II again for 15 minutes, then take out the slices and place them in absolute ethanol I for 5 minutes min, take it out and put it in absolute ethanol II again for 5 min, take it out and put it in 85% ethanol for 5 min, then take it out and put it in 75% ethanol for 5 min, and finally take out the slice and wash it in distilled water for 5 min;

[0023] 4. Antigen retrieval: Place tissue sections in a repair box filled with pH 9.0 EDTA antigen retrieval buffer and carry out antigen retrieval in a microwave oven. During th...

Embodiment 2

[0032] Example 2 The relationship between the expression level of PITX1 and the prognosis of gastric cancer

[0033] Using the method disclosed in Example 1, the expression levels of PITX1 in 174 gastric cancer patients were detected, wherein negative, false positive and weak positive were low expression groups, positive and strong positive were high expression groups, according to Kaplan-Meier (KM ) to analyze the correlation between PITX1 low expression and high expression groups and prognosis, the results are as follows figure 1 .

[0034] The results showed that PITX1 was significantly correlated with prognosis, that is, the stronger the expression of PITX1, the better the prognosis of the patient, on the contrary, the weaker the expression of PITX1, the worse the prognosis.

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Abstract

The invention relates to application of a reagent for detecting PITX1 (paired-like homeodomain 1) expression in the preparation of a gastric cancer prognosis evaluation kit, belongs to the technical field of biology and particularly relates to the field of medical diagnosis. The invention discloses application of a reagent for detecting PITX1 expression in the preparation of a gastric cancer prognosis evaluation kit. Predicted gastric cancer prognosis and chemotherapy resistance condition can be acquired by marking gastric cancer prognosis prediction and gastric cancer chemotherapy evaluation, and by using immunohistochemical method to detect the expression level of PITX1 gene; the PITX1 gene is simple to introduce; the whole predictive evaluation is simple and easy and is highly feasible.

Description

technical field [0001] The invention relates to the application of a reagent for detecting the expression level of PITX1 in the preparation of a gastric cancer prognosis assessment kit, belonging to the field of biotechnology, in particular to the field of medical diagnosis. Background technique [0002] Gastric cancer is a disease that seriously endangers the health of our people. So far, there is no better targeted drug, resulting in poor treatment effect and poor prognosis for patients. In addition, gastric cancer is a highly heterogeneous disease, and different patients have different etiologies, resulting in differences in diagnosis and treatment. In particular, different genetic bases may cause differences in the efficacy of chemotherapy drugs in patients. At present, there is no technical means at home and abroad that can effectively judge the prognosis of the differences in the efficacy of chemotherapy drugs. [0003] Establishing effective predictive markers for...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57446
Inventor 樊红乔凤昌沈小慧李懿萍
Owner SOUTHEAST UNIV
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