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Keratin nanomaterials and methods of production

A technology of nanomaterials and keratin, which is applied in the preparation and application of keratin nanomaterials, macromolecular keratin complexes, and keratin nanomaterials, and can solve the problem of low immunogenicity

Inactive Publication Date: 2017-08-01
VIRGINIA TECH INTPROP INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Finally, biomaterials made of keratin nanomaterials are less immunogenic because of the absence of damaged peptides and because of the intact native structure of molecular complexes of type I and type II monomers

Method used

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  • Keratin nanomaterials and methods of production
  • Keratin nanomaterials and methods of production
  • Keratin nanomaterials and methods of production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1: prepare oxidized keratin nanomaterial

[0096] Samples of Chinese hair were obtained from commercial suppliers and used directly without treatment. 100 g of hair fibers were placed into 2 liters of 2% w / v peracetic acid and shaken at 150 rpm for 8 hours at 37°C. The oxidized hair was recovered through a sieve and extracted with 4 liters of 100 mM Tris base by shaking at 150 rpm for 15 hours at 37°C. The hair was recovered through a sieve, the extract solution was retained, and the hair fiber was further extracted using 4 liters of pure water by shaking at 150 rpm for 2 hours at 37°C. Recover the hair with a sieve and discard, reserving the extract solution. The two extract solutions are combined to form a solution of crude keratin extract which is purified of particulate matter by centrifugation through a solid separator operating at 40000 rpm, followed by filtration through a membrane with an average pore size of 20-25 microns . Keratin nanomaterial...

Embodiment 2

[0099] Embodiment 2: prepare oxidized keratin nanomaterial

[0100] Samples of Chinese hair were obtained from commercial suppliers and used directly without treatment. 200 g of hair fibers were placed into 4 liters of 2% w / v peracetic acid and shaken at 125 rpm for 8 hours at 37°C. The oxidized hair was recovered through a sieve and extracted using 8 liters of 100 mM Tris base by shaking at 125 rpm for 14 hours at 37°C. The hair was recovered through a sieve, the extract solution was retained, and the hair fiber was further extracted using 8 liters of pure water by shaking at 125 rpm for 2 hours at 37°C. Recover the hair with a sieve and discard, reserving the extract solution. The two extract solutions were combined to form a solution of crude keratin extract that was purified of particulate matter by centrifugation through a solid separator operating at 43,000 rpm, followed by centrifugation through a membrane with an average pore size of 20-25 microns. filter. Kerati...

Embodiment 3

[0103] Embodiment 3: preparation reduces keratin nanomaterial

[0104] Samples of Chinese hair were obtained from commercial suppliers and used directly without treatment. Keratin extraction was achieved by a multi-step reduction process as follows: 100 g of hair was placed in 2 liters of a 0.5 M thioglycolic acid (TGA) solution adjusted to a pH of 10.5 and heated at 150 rpm at 37° C. Shake for 15 hours. The hair is recovered with a sieve, and the extraction solution is retained. Then, the hair fibers were placed in 4 liters of a solution of 100 mM Tris and shaken at 150 rpm for 2 hours at 37°C. The resulting extraction solution was kept, and the hair was placed in 4 liters of pure water and shaken at 150 rpm for 2 hours at 37°C. The hair was recovered with a sieve, placed in 1 liter of a freshly prepared 0.5 M TGA solution adjusted to a pH of 10.5, and shaken at 150 rpm for 15 hours at 37°C. The hair is recovered with a sieve, and the extraction solution is retained. T...

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Abstract

The present disclosure relates to keratin nanomaterials, methods for obtaining keratin nanomaterials, and biomaterials made from keratin nanomaterials. In particular, keratin nanomaterials comprising Type I and Type II monomer pairs are disclosed as well as a method for obtaining keratin nanomaterials comprising obtaining a solution of keratin and processing the solution by ultrafiltration with buffer solution containing phosphate.

Description

[0001] Cross References to Related Applications [0002] This application claims priority and benefit to U.S. Provisional Patent Application No. 61 / 987,182, filed May 1, 2014, and U.S. Provisional Patent Application No. 61 / 987,855, filed May 02, 2014, all of which are adopted by incorporated by reference. [0003] field of invention [0004] The present invention generally relates to the field of keratin nanomaterials, biomaterials comprising keratin nanomaterials, and methods for preparing keratin nanomaterials. Specifically, the present invention relates to the preparation and application of keratin nanomaterials, which are macromolecular keratin complexes comprising dimers and tetramers of tightly bound keratin monomers. [0005] Background of the invention [0006] Intermediate fibers (IFs) contain subunit assemblies belonging to the α-helical protein superfamily belonging to one of six major classes. In general, IF proteins have shared secondary structural features that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17
CPCA61L27/227A61L15/32B82Y5/00A61L24/108A61L26/0047A61L2400/12C07K1/34C07K14/4741
Inventor M·范 戴克M·B·拉夫曼妮
Owner VIRGINIA TECH INTPROP INC
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