Embryo culture unit and manufacturing method thereof

Abstract

The invention belongs to the technical field of embryo culture, and relates to an embryo culture unit. The embryo culture unit comprises a culture unit body, wherein the culture unit body is made of transparent materials; a sample feeding end plug hole and a sampling end plug hole are formed in the upper surface of the culture unit body, and are sealed by plugs; a sample feeding hole is formed in the bottom part of the sample feeding end plug hole, and a sampling hole is formed in the bottom part of the sampling end plug hole; an embryo containing cavity is arranged just under the sampling hole; an embryo culture cavity is arranged in the culture unit body, and is communicated with the sample feeding hole through a sample feeding channel; the embryo containing cavity is communicated with the embryo containing cavity through a sample discharging channel. The embryo culture unit has the advantages that the embryo culture unit is filled with a one-travel type culture medium which is pre-balanced by carbon dioxide, then an absolutely sealed embryo in-vitro culture system is formed, the in-vitro development of oosperm to the blastocyst stage can be directly supported in a common constant-temperature box at temperature of 37 DEG C, and the carbon dioxide culture box is not needed; the development of embryo is conveniently observed, the culture step is greatly simplified, and the working efficiency and quality are improved.

Description

technical field [0001] The invention belongs to the technical field of embryo culture equipment, and relates to an embryo culture unit and a manufacturing method thereof. Background technique [0002] The current in vitro fertilization and embryo culture are all completed in a carbon dioxide incubator, relying on the interaction and communication between the constant temperature and constant concentration of carbon dioxide in the incubator and the chemical components in the medium to maintain the sperm and egg fertilization and embryo growth. physiological environment. However, there are some defects or deficiencies in the existing culture system, whether in the hardware equipment (cultivation device or incubator) or in the culture medium. As far as the hardware is concerned, the existing culture device (incubator) is an open system. The gas in the carbon dioxide incubator needs to communicate with the air outside the box, that is, the embryo laboratory, to maintain the gas...

AI Technical Summary

Problems solved by technology

As far as the hardware is concerned, the existing culture device (incubator) is an open system. The gas in the carbon dioxide incubator needs to communicate with the air outside the box, that is, the embryo laboratory, to maintain the gas in the incubator or the device. Therefore, such an open system has the following obvious deficiencies or defects: First, the gas concentration and temperature in the device are actually in a dynamic equilibrium state, and the gas concentration and temperature fluctuate within a certain range. The size of the fluctuation depends on the type and quality of the device or incubator and the frequency of opening the door of the incubator; second, volatile organic compounds and harmful physical and chemical factors such as ozone in the laboratory can enter into the laboratory through this open gas exchange system. In the carbon dioxide incubator, it will cause damage to the embryo, or even death; the third is that when the door of the carbon dioxide incubator is opened, the gas in the incubator will overflow a large amount, which will inevitably cause the gas concentration and temperature in the incubator to fluctuate violently. The degree of embryo damage caused by such violent fluctuations also increases

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