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A method and system for str typing individuals

An individual and systematic technology, applied in the field of STR typing and system for individuals, can solve the problems of huge differences in ethnic genome polymorphisms and cannot be directly applied to Asian populations, and achieve increased inspection costs, uniform arrangement, and high individual identification Effect

Active Publication Date: 2020-12-15
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are huge differences in genomic polymorphisms in different regions and ethnic groups. The existing foreign databases and the core loci for individual identification and infringement identification cannot be directly applied to Asian populations. Establish a database of its own characteristics and determine the core loci that can be used for individual identification and infringement identification

Method used

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  • A method and system for str typing individuals
  • A method and system for str typing individuals
  • A method and system for str typing individuals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1, the verification of the accuracy of the method and system for carrying out STR typing of the present invention

[0053] In this example, 200 samples of human venous blood are described, and their individual sources are known, but in the implementation of Example 1 of this application, their individual sources are set to be unknown, and the method and system of this application are used for STR analysis. type, including:

[0054] 1) Using the DNA acquisition system in the system of the present invention to obtain the individual DNA, 2) Using the composite detection system in the system of the present invention to obtain the genotypes of the 19 STR loci of the individual DNA, and obtain according to the genotypes The STR typing result of the individual, 3) using the inference system in the system of the present invention, carry out individual identification, family investigation or paternity identification according to the STR typing result of the individual...

Embodiment 2

[0083] Example 2 Sensitivity, species specificity, system repeatability, accuracy verification of typing results, and detection of anti-inhibition ability of the complex amplification system of the present invention

[0084] 1. Sensitivity detection

[0085] When the template amount is ≥0.125ng, all alleles of the 19 loci can be correctly typed; if the template amount is figure 2 . It can be seen that the system of the present invention can detect as little as 0.125 ng of DNA.

[0086] 2. Species Specific Detection

[0087] The complex amplification system detected animal blood samples, and there was no specific peak type in the typing area, as shown in Figure 3. It can be seen that the method and system of the present invention have good species specificity.

[0088] 3. System repeatability detection

[0089] 2 batches of reagents were used to test 92 samples (venous blood, from the Physical Evidence Identification Center of the Ministry of Public Security), and the resu...

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Abstract

The invention provides a method and a system for performing STR genotyping on individuals. The method comprises the following steps: obtaining DNA of the individuals, wherein the obtained DNA of the individuals comprises a genotype with 19 gene loci comprising 18 autosome STR gene loci and a sex-determining gene locus Amelogenin, wherein the 18 autosome STR gene loci refer to D18S51, D21S11, D3S1358, FGA, D8S1179, vWA, CSF1PO, D16S539, D7S820, D13S317, D5S818, D2S1338, D12S391, TH01, TPOX, D19S433, Penta E and D6S1043; and obtaining the STR genotyping results of the individuals according to the genotype of the 19 gene loci. According to the scheme, the normal checked materials and trace degradation checked materials of individuals of Chinese population can be subjected to genotyping, and a data support is provided for realizing individual recognition, family checking or paternity identification and the like.

Description

technical field [0001] The present invention relates to a typing method and system, in particular to a method and system for performing STR typing on individuals. Background technique [0002] Since the advent of DNA testing technology, especially STR testing technology in the mid-1980s, it has been widely used in criminal and civil cases, large-scale catastrophic accidents (such as: 9.11 terrorist attacks in the United States, Indonesian tsunami, Wenchuan Earthquake) and mass emergencies. Both played a huge role. With the development of microbiological evidence DNA testing technology and short fragment STR composite amplification testing technology, the application scope of DNA testing technology has a tendency to further expand, and the continuous expansion of DNA database construction scale has made cross-regional mobile crimes and series of cases And detection has become a long-term mechanism. In the anti-trafficking special operation in 2009, the Ministry of Public Se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/156C12Q2600/16
Inventor 白雪姚伊人李万水叶健莫晓婷马温华
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY