Tissue culture method and rooting medium for Ginkgo biloba

A technology for rooting medium and tissue culture, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve the problems of tissue culture seedlings browning, easy pollution, etc. The effect of time to promote germination

Inactive Publication Date: 2017-08-18
广安职业技术学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] This application provides a tissue culture method and rooting medium for ginkgo to solve the technical problems of serious browning of tissue culture seedlings and easy pollution in the existing technology of promoting ginkgo reproduction through tissue culture

Method used

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  • Tissue culture method and rooting medium for Ginkgo biloba
  • Tissue culture method and rooting medium for Ginkgo biloba
  • Tissue culture method and rooting medium for Ginkgo biloba

Examples

Experimental program
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Effect test

Embodiment 1

[0091] Select the side bud stem tips with 2-3 leaves of the mother tree with 2-3 leaves from late March to early May in Guang'an area as explants. Soak the collected explants in washing powder water for 1 hour, rinse with running water for 5 minutes; then soak them in tap water for 4-5 times; place the obtained explants in 70% ethanol solution for 1 minute, and wash them with sterile water for 3 times , treated with 0.5% bromogeramine for 15 minutes, and washed 3-4 times with sterile water. The explant after the disinfection treatment is inoculated in the rooting medium, the mass ratio of each raw material in the rooting medium is: MS medium mother liquor: sucrose: Ginkgo biloba gac: naphthalene acetic acid: 6-benzylaminopurine: agar=1000000: 15000:2000:0.15:1:8000. The pH of the medium was 5.2. Place the inoculated culture bottle under the conditions of 1000lux light, light 10h / d, and 20°C for cultivation.

Embodiment 2

[0093]Select the side bud stem tips with 2-3 leaves of the mother tree with 2-3 leaves from late March to early May in Guang'an area as explants. Soak the collected explants in washing powder water for 2 hours, rinse with running water for 10 minutes; then soak them in tap water for 4-5 times; place the obtained explants in 70% ethanol solution for 1 minute, and wash them with sterile water for 3 times , treated with 0.5% bromogeramine for 20 minutes, and washed 3-4 times with sterile water. The explant after the disinfection treatment is inoculated in the rooting medium, the mass ratio of each raw material in the rooting medium is: MS medium mother liquor: sucrose: Ginkgo biloba gac: naphthalene acetic acid: 6-benzylaminopurine: agar=1000000: 75000:4000:0.15:1:7500, the pH value of the medium is 5.4. Place the inoculated culture bottle under the conditions of 1000lux light, light 10h / d, and 20°C for cultivation.

Embodiment 3

[0095] Select the side bud stem tips with 2-3 leaves of the mother tree with 2-3 leaves from late March to early May in Guang'an area as explants. Soak the collected explants in washing powder water for 3 hours, rinse with running water for 15 minutes; then soak them in tap water for 4-5 times; place the obtained explants in 70% ethanol solution for 2 minutes, and wash them with sterile water for 3 times , treated with 0.5% bromogeramine for 25 minutes, and washed 3-4 times with sterile water. The explant after the disinfection treatment is inoculated in the rooting medium, the mass ratio of each raw material in the rooting medium is: MS medium mother liquor: sucrose: Ginkgo biloba gac: naphthalene acetic acid: 6-benzylaminopurine: agar=1000000: 0:6000:0.15:1:7000, the pH value of the medium is 5.6. Place the inoculated culture bottle under the conditions of 1000lux light, light 10h / d, and 20°C for cultivation.

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Abstract

The embodiment of the application provides a tissue culture method and a rooting medium for Ginkgo biloba. The method comprises the steps that agar, sucrose, an MS medium mother liquid, a 6-benzylaminopurine solution and a Naphthylacetic acid solution, and Ginkgo biloba branch activated carbon or Ginkgo biloba husk activated carbon are taken as raw materials to prepare the rooting medium; a stem tip with leaves of a Ginkgo biloba lateral bud is taken as explant, the explant is inoculated to the rooting medium after disinfection treatment, and the explant is cultivated with illumination of 500-1000 lux and at the temperature of 20-25 DEG C. According to the technical scheme, the stem tip with leaves of the Ginkgo biloba lateral bud is taken as the explant, and rooting is directly induced, so that the seedling period is shortened; good traits of a parent can be retained to the utmost extent; the Ginkgo biloba branch activated carbon or the Ginkgo biloba husk activated carbon is added into the rooting medium, which can improve the browning phenomenon of tissue culture seedlings and have the effects of anti-bacteria and growth promotion at the same time.

Description

technical field [0001] The application relates to the technical field of tissue culture, in particular to a tissue culture method and rooting medium of ginkgo biloba. Background technique [0002] Ginkgo is a deciduous tree belonging to the Ginkgo family and the genus Ginkgo. It is a unique tree species in my country. Ginkgo appeared hundreds of millions of years ago. It is the oldest relic plant among the gymnosperms left over from the Quaternary glacial movement. The surviving Ginkgo is rare and scattered. , Hundred-year-old trees are rare, and all other plants in the same class are extinct, so ginkgo is also known as a "living fossil". Ginkgo biloba branches grow slowly and have a very long life span. Under natural conditions, it takes more than 20 years from planting to ginkgo fruiting, and it takes 40 years for a large number of fruiting. Ginkgo has the functions of greening the environment, purifying the air, maintaining water and soil, controlling insect pests, regula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 喻娜向丹崔帅赵静
Owner 广安职业技术学院
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