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Preparation method and application of solid lactobacillus reuteri micro-ecological preparation

A technology of Lactobacillus reuteri and microecological preparations, applied in the field of probiotics, can solve the problems of not considering homologous screening and homologous feeding of strains, less consideration of action objects, hindering addition, etc., to achieve reduction The frequency and cost of artificial insemination, the effect of reducing the incidence of diarrhea and reducing the incidence of miscarriage

Active Publication Date: 2017-08-18
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

③After the living bacteria enter the digestive tract, most of them are not easy to tolerate the action of gastric acid and bile acid, and it is difficult for a sufficient number to reach the intestinal tract to play a role, so they cannot play a good role in inhibiting pathogenic bacteria
④ The application of microecological preparations lacks pertinence, less consideration is given to the target of action, homologous screening and homologous feeding of strains are not considered
⑤ Insufficient safety, some bacillus such as Bacillus cereus can contaminate food and produce toxins, causing food poisoning
[0008] In reality, lactic acid bacteria preparations are mostly liquid, and the number of live bacteria contained in them does not meet the requirements.
This not only hinders the addition in the feed, but also fails to achieve the effect of use

Method used

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Embodiment 1

[0037] A preparation method of solid-state Lactobacillus reuteri probiotics, the specific steps are as follows:

[0038] (1) Selection of strains

[0039] Select Lactobacillus reuteri (patent preserved by China Type Culture Collection Center (Wuhan University Collection Center), the preservation number is CCTCC M 2015660) and store it at 4°C for later use;

[0040] (2) Preparation of MRS medium

[0041] 10.0g peptone, 10.0g beef extract, 5.0g yeast extract, 20.0g glucose, 1.0mL Tween 80, 2.0g dipotassium hydrogen phosphate, 5.0g sodium acetate, 2.0g diammonium hydrogen citrate, 0.58g magnesium sulfate, 0.25g of manganese sulfate and 1000mL of distilled water are heated and dissolved, and the pH value is adjusted to 6.7. After subpackaging, sterilize by high-pressure steam at 121°C for 20 minutes, and set aside.

[0042] (3) Activation of strains and acquisition of seed solution

[0043] Inoculate the Lactobacillus reuteri stored at 4°C on the MRS medium, culture at 37°C for...

Embodiment 2

[0054] Experimental animals and groups

[0055] Choose 80 healthy, uniform weight sows (sows with similar parity), and the basic diet is based on the standards of the National Research Council of the United States (NRC, 1998) and the agricultural industry standards of the People's Republic of China (Pig Standards, NY / T65-2004 ) sows with nutritional requirements, the basic diet composition and nutritional levels are shown in Table 1. Divide into 4 groups at random (every 20), and I, II, III group are respectively the probiotic lactobacillus group purchased on the market, the solid-state Lactobacillus reuteri microecological preparation group of the present invention, the composite probiotic group purchased on the market, The addition amount is 0.5%, so that the number of viable bacteria in the basal diet reaches 1.0×10 7 CFU / mL, see Table 2. Feeding method: Mix the probiotic preparation into the basal diet, and feed each head in a single pen. The feeding cycle is the entire ...

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Abstract

The present invention relates to a preparation method of a solid lactobacillus reuteri micro-ecological preparation. The method comprises the following specific steps: a MRS medium is prepared for a standby application; lactobacillus reuteri is inoculated on the MRS medium, the lactobacillus reuteri is cultured at 37 DEG C for 30-36 h, continuous activations are conducted for 3-5 times, then the activated lactobacillus reuteri is inoculated in the MRS medium, and a strain of a lactobacillus reuteri seed solution is obtained by the culture at 37 DEG C for 24 hours; soybean meal, soybean flour, corn flour, wheat bran, betaine and mannose are mixed evenly to obtain a solid medium; the seed liquid is inoculated to the solid medium, and the solid fermentation is conducted at 37 DEG C for 24-28 hours; and the product is dried, the dried product is bagged, and the bagged product is sealedly preserved. In the product, the viable count of the lactobacillus reuteri reaches 3.2+ / -0.5x10<9> CFU / g. The product is used as a feed additive for pigs, lowers incidence of sow abortion, reduces frequency and cost of artificial insemination, reduces incidence of diarrhea and mortality of suckling piglets, etc.

Description

technical field [0001] The invention belongs to the field of microecological preparations, and in particular relates to a preparation method and application of a solid Lactobacillus reuteri microecological preparation. Background technique [0002] As a new type of non-toxic and non-polluting microbial product feed additive, microecological preparations can regulate the microecological balance of animals, prevent diseases, improve feed conversion rates, improve animal production performance and protect the ecological environment. substitute role. [0003] Lactic acid bacteria are the most important source of probiotics, and the application of lactic acid bacteria in animal production and the exploration of related mechanisms are gradually deepening. Lactic acid bacteria can adjust the flora balance of the gastrointestinal tract, neutralize the toxins produced by pathogenic bacteria, and reduce the pH value of the intestine. In addition to producing antibacterial substances,...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K40/00A23K50/30
Inventor 杨家军钱坤吴义景陈胜徐晓英徐雅芫
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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