Novel method for storing marine organism specimen by using epoxy resin

A technology of epoxy resin, marine life

Inactive Publication Date: 2017-08-18
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The present invention aims at the above-mentioned technical problems such as complex operation and lack of wide applicability in the preparation of aquatic biological specimens, and proposes a scientific method, convenient operation, long storage time, good preservation effect, and convenient transportation using epoxy resin to preserve biological specimens new method of

Method used

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  • Novel method for storing marine organism specimen by using epoxy resin
  • Novel method for storing marine organism specimen by using epoxy resin
  • Novel method for storing marine organism specimen by using epoxy resin

Examples

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Embodiment 1

[0031] Example 1, this example provides a method for making a biological specimen that does not require staining

[0032] Firstly, the organisms that need to be made into specimens and have been fixed are directly put into absolute ethanol or acetone for dehydration. For small and tiny aquatic organisms, the dehydration time is very short. Probably ranging from tens of minutes to several days. Afterwards, the specimen is taken out and placed in a container. During this process, if the body of the specimen is very soft, it may shrink during the dehydration step. In order to prevent deformation during the dehydration process, gradient dehydration can be adopted, or the dehydration time can be shortened.

[0033] Then, mix the epoxy resin and curing agent according to the weight ratio of 3:1. In this embodiment, the epoxy resin is Nanya 128 epoxy resin (E-51), the viscosity is 12000-15000, and the epoxy equivalent is 184-190. It is a liquid bisphenol A resin. The viscosity is...

Embodiment 2

[0036] Embodiment 2, the present embodiment provides a kind of preparation method of the marine biological specimen stained with Coomassie Brilliant Blue staining solution

[0037] Firstly, the organisms to be made into specimens are directly put into absolute ethanol for dehydration. For small and tiny aquatic organisms, the dehydration time is very short. Probably ranging from tens of minutes to several days. Afterwards, the specimen is taken out and placed in a container. During this process, if the body of the specimen is very soft and easy to shrink, in order to prevent deformation during the dehydration process, gradient dehydration can be adopted, or the dehydration time can be shortened.

[0038] Weigh 100mg of Coomassie Brilliant Blue G-250 and dissolve it in 50mL of 90% ethanol, add 100mL of 85% phosphoric acid, and finally distill the volume to 1000mL with distilled water, then let the solution stand at room temperature for one month to obtain Coomassie Brilliant ...

Embodiment 3

[0043] Embodiment 3, the present embodiment provides a kind of preparation method of the marine biological specimen stained with eosin staining solution

[0044] Firstly, the aquatic organisms that need to be made into specimens are directly put into absolute ethanol for dehydration. For small and tiny aquatic organisms, the dehydration time is very short. Probably ranging from tens of minutes to several days. Afterwards, the specimen is taken out and placed in a container. During this process, if the body of the specimen is very soft, it is easy to shrink during the dehydration step. In order to prevent deformation during the dehydration process, gradient dehydration can be adopted, or the dehydration time can be shortened.

[0045] Then, add 0.1 g of alcohol-soluble eosin into 100 mL of 95% ethanol, heat to about 40° C., and stir until it is completely dissolved. Cool down to room temperature to make eosin staining solution.

[0046] Then the above-mentioned dehydrated o...

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Abstract

The invention belongs to the field of preparation methods of organism specimens, relates to an aquatic organism specimen and particularly relates to a novel method for storing an aquatic organism specimen by using epoxy resin. The method comprises the following effective steps of (a) dehydration, (b) resin preparation; (c) embedding; and (d) solidification. By using mixed preparation of the epoxy resin and a curing agent, the whole aquatic organism specimen is simple and fast in preparation and low in cost; the specimen produced by using the method can adapt to the research requirements of an organism teaching tool and an organism specimen of a standard sample. Meanwhile, the organism specimen prepared through the method is convenient to store, transport, exchange and communicate, the classification and identification characteristics of microorganisms are well stored, and the organism specimen is not easy to age and is especially suitable for storage of small and miniature aquatic organisms.

Description

technical field [0001] The invention belongs to the field of preparation methods of biological specimens, and relates to marine and freshwater biological specimens. Background technique [0002] At present, the long-term preservation of miniature and small aquatic organisms is preserved by soaking in formalin solution and sealed by natural resin. Although above-mentioned two kinds of methods can obtain the specimen of aquatic organisms, it still has the following problems: first, the small-scale aquatic organisms preserved with formalin solution has strong smell, is not suitable for teaching aids as students. At the same time, the soaked specimen needs to be poured out for observation. For tiny samples, the operation process is cumbersome, and the specimen is easily lost, which brings inconvenience to teaching and scientific research. However, if the classic resin mounting method is used, due to the high water content of aquatic organisms, it is difficult for the resin to p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 王敏晓程方平何青
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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