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A high-precision identification method for the optimal excitation angle position of two-dimensional surface plasmons

An identification method and technology of excitation angle, which is applied in the direction of material excitation analysis, measuring device, instrument, etc.

Inactive Publication Date: 2019-07-05
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to solve the problem of accurate identification of the optimal excitation angle position of two-dimensional SPR, the present invention provides a high-precision automatic identification method for the optimal excitation angle position of two-dimensional SPR

Method used

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  • A high-precision identification method for the optimal excitation angle position of two-dimensional surface plasmons
  • A high-precision identification method for the optimal excitation angle position of two-dimensional surface plasmons
  • A high-precision identification method for the optimal excitation angle position of two-dimensional surface plasmons

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specific Embodiment approach 1

[0033] Specific embodiment one: when using the incident light of linear polarization mode to excite SPR, the optimal excitation angle of SPR obtained on the image sensor conjugated with the rear focal plane of the microscope objective is characterized in that a pair of Symmetrical SPR absorption arc. The precise fitting of the clear aperture boundary and the optimal excitation angle position for the SPR is performed as follows:

[0034] Step 1: Input a frame of rectangular image containing linearly polarized back focal plane information, the image size is n×m, normalize the pixel value to [0,1], and get the following image 3 image shown.

[0035] Step 2: For the original image data of the example, the brightness is uneven, one side is brighter, and the other side is darker. When using the global threshold value to binarize the image, if the threshold value is small, the boundary of the dark side of the clear aperture is easily confused with the background It is difficult to...

specific Embodiment approach 2

[0055] Specific embodiment two: when using the incident light of the radial polarization mode to excite the SPR, the optimal excitation angle of the SPR obtained on the image sensor conjugated to the rear focal plane of the microscope objective is characterized in that a Full circle of SPR absorption. The precise fitting of the clear aperture boundary and the SPR optimal excitation angle position is performed as follows:

[0056] Input a frame of rectangular image containing radially polarized back focal plane information, normalize the pixel values ​​to [0,1], and get the following Figure 11 image shown. Take steps 2-step 7 in the specific embodiment 1 to process the image, wherein the processing of steps 5-step 7 is the processing of the absorption ring corresponding to the best excitation angle of the SPR in the clear aperture, so as to extract the radial The precise center and radius of the optimal excitation angle of SPR under polarization, as attached Figure 12 show...

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Abstract

The invention discloses a high-precision identification method for an optimal excitation angle position of two-dimensional surface plasma. The method comprises the following steps: roughly positioning the position of a clear aperture circle through local binarization and Hough transform, and cutting an objective rear focal plane image acquired by an image sensor conjugated with an objective rear focal plane into a W*W square matrix image comprising the clear aperture circle; increasing a contrast ratio of clear aperture to a clear aperture external part through local binarization, and detecting the circle center and the radius of the clear aperture; counting grey value distribution in the radius direction in the clear aperture, and estimating the rough radius of a circle on which the SPR optimal excitation angle is located; determining grey distribution of pixels nearby the SPR optimal excitation angle by combining with a morphological method; and finally, calculating the circle center and the radius of the circle on which the optimal excitation angle is calculated through a least square method, thereby obtaining the precision position of the SPR optimal excitation angle.

Description

technical field [0001] The invention relates to the technical field of nanometer optical detection, in particular to a method for feature extraction of two-dimensional surface plasmon optimal excitation angle. Background technique [0002] Surface plasmon (Surface Plasmon Resonance, SPR) is a surface electromagnetic wave formed by the free electron flow on the metal surface under the action of an external electric field such as electron flow or incident light field. Accurate quantitative characterization has a wide range of applications in biomedicine, materials, chemistry and other fields. Commonly used SPR coupling methods include prism coupling and high numerical aperture (NA) oil immersion microscope objective coupling. Among them, the optimal excitation angle feature of SPR corresponding to prism coupling SPR technology is usually a black light band, and the identification method is simple and has been established. relatively mature. Microscopic objective coupled SPR ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 张蓓王秋生陈璐张承乾刘雨闫鹏
Owner BEIHANG UNIV