Preparation method and application of a slow-release enzyme-bacteria mixture for in-situ repair of polluted water
A slow-release enzyme bacteria, in-situ repair technology, applied in the field of environmental management, can solve the problems of easy loss of microbial activity, poor microbial adaptability, and high requirements for bacterial species, and achieve the effect of increasing the repair effect and reducing the use.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0024] The preparation method of the slow-release enzyme bacteria mixture for in-situ repair of polluted water body of the present embodiment comprises the following steps:
[0025] (1) Preparation of compound bacterial liquid: Inoculate Bacillus lateralosporus, photosynthetic bacteria, Lactobacillus acidophilus, and saccharomyces into LB medium respectively, culture on a shaker at 28-30 degrees for 20-30 hours, and press 1 after obtaining the corresponding bacterial liquid : 1:1:1 volume ratio mixed to make a composite bacterial liquid;
[0026] (2) Prepare compound enzyme liquid: mix pineapple enzyme, papain, lysozyme, cellulase and distilled water to make compound enzyme liquid, the content of pineapple enzyme in the compound enzyme liquid is 50mg / L, the content of papain is 35mg / L L, the content of lysozyme is 20mg / L, the content of cellulase is 50mg / L.
[0027] (3) Preparation of slow-release enzyme-bacteria mixture: compound bacteria liquid, composite enzyme liquid, sta...
Embodiment 2
[0030] The preparation method of the slow-release enzyme bacteria mixture for in-situ repair of polluted water body of the present embodiment comprises the following steps: the preparation method of the slow-release enzyme bacteria mixture for in-situ repair of polluted water body of the present embodiment comprises the following steps:
[0031] (1) Preparation of compound bacterial liquid: inoculate Bacillus lateralosporus, photosynthetic bacteria, Lactobacillus acidophilus, and saccharomyces into LB medium respectively, and cultivate on a shaker at 28-30 degrees for 20-30 hours, and press 2 after obtaining the corresponding bacterial liquid : 1:5:1 volume ratio mixed to make a composite bacterial liquid;
[0032] (2) Prepare compound enzyme liquid: mix pineapple enzyme, papain, lysozyme, cellulase and distilled water to make compound enzyme liquid, the content of pineapple enzyme in the compound enzyme liquid is 30mg / L, the content of papain is 25mg / L L, the content of lysoz...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com