Preparation method and application of a slow-release enzyme-bacteria mixture for in-situ repair of polluted water
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A slow-release enzyme bacteria, in-situ repair technology, applied in the field of environmental management, can solve the problems of easy loss of microbial activity, poor microbial adaptability, and high requirements for bacterial species, and achieve the effect of increasing the repair effect and reducing the use.
Active Publication Date: 2021-06-22
ZHEJIANG AVOCADO ENVIRONMENTAL PROTECTION TECH CO LTD
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Problems solved by technology
At present, there are commonly used technologies such as planting aquatic plants and bacteriostasis, but the maintenance cost of aquatic plants in the later stage of planting is high, and the water quality is affected by the dry season of plants; Easy to lose, need to invest repeatedly
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Embodiment 1
[0024] The preparation method of the slow-release enzyme bacteria mixture for in-situ repair of polluted water body of the present embodiment comprises the following steps:
[0025] (1) Preparation of compound bacterial liquid: Inoculate Bacillus lateralosporus, photosynthetic bacteria, Lactobacillus acidophilus, and saccharomyces into LB medium respectively, culture on a shaker at 28-30 degrees for 20-30 hours, and press 1 after obtaining the corresponding bacterial liquid : 1:1:1 volume ratio mixed to make a composite bacterial liquid;
[0026] (2) Prepare compound enzyme liquid: mix pineapple enzyme, papain, lysozyme, cellulase and distilled water to make compound enzyme liquid, the content of pineapple enzyme in the compound enzyme liquid is 50mg / L, the content of papain is 35mg / L L, the content of lysozyme is 20mg / L, the content of cellulase is 50mg / L.
[0030] The preparation method of the slow-release enzyme bacteria mixture for in-situ repair of polluted water body of the present embodiment comprises the following steps: the preparation method of the slow-release enzyme bacteria mixture for in-situ repair of polluted water body of the present embodiment comprises the following steps:
[0031] (1) Preparation of compound bacterial liquid: inoculate Bacillus lateralosporus, photosynthetic bacteria, Lactobacillus acidophilus, and saccharomyces into LB medium respectively, and cultivate on a shaker at 28-30 degrees for 20-30 hours, and press 2 after obtaining the corresponding bacterial liquid : 1:5:1 volume ratio mixed to make a composite bacterial liquid;
[0032] (2) Prepare compound enzyme liquid: mix pineapple enzyme, papain, lysozyme, cellulase and distilled water to make compound enzyme liquid, the content of pineapple enzyme in the compound enzyme liquid is 30mg / L, the content of papain is 25mg / L L, the content of lysoz...
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Abstract
The invention discloses a preparation method of a slow-release enzyme bacteria mixture for in-situ restoration of polluted water, which comprises the following steps: (1) preparing a composite bacteria liquid: separately mixing Bacillus lateralosporus, photosynthetic bacteria, Lactobacillus acidophilus and yeast Inoculate in LB medium for cultivation, obtain the corresponding bacterial liquid and mix to make a compound bacterial liquid; (2) prepare a compound enzyme liquid: mix pineapple enzyme, papain, lysozyme, cellulase and distilled water to make a compound enzyme liquid; (3) Preparation of the slow-release enzyme-bacteria mixture: compound the compound bacteria liquid, the composite enzyme liquid, the adsorbent, and honey into a mixture, and mold it into a ball machine to make the slow-release enzyme-bacteria mixture. The slow-release enzyme-bacteria mixture prepared by the present invention can degrade the nutrient-rich ammonia nitrogen and phosphorus in the polluted water body, inhibit the growth of harmful microorganisms, and also have effects such as biological dredging, and because the enzyme-bacteria mixture of the present invention has a slow-release effect, it can be applied to In the restoration of polluted water body, the effect of water body treatment can be exerted gradually, and there is no need to repeatedly put it in.
Description
technical field [0001] The invention relates to the field of environmental treatment, in particular to a preparation method and application of a slow-release enzyme-bacteria mixture for in-situ restoration of polluted water. Background technique [0002] my country's economy is developing at a high speed, but the pollution that comes with it is also very distressing. According to the 2013 China Environmental Bulletin, among the nationally controlled sections of the Yangtze River, the Yellow River, the Pearl River, the Songhua River, the Huaihe River, the Haihe River, the Liaohe River, the rivers in Zhejiang and Fujian, the rivers in the northwest and the rivers in the southwest, the grades IV-V and inferior to V The proportions of water-like sections are 19.3% and 9.0% respectively. The main pollution indicators are chemical oxygen demand, permanganate index and five-day biochemical oxygen demand. 18.2% and 19.5% of the cross-sections with water quality at the provincial b...
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