Capillary chemiluminiscence detection device and detection method thereof

Abstract

The invention discloses a capillary chemiluminiscence detection device and a detection method thereof and solves the problems of high cost, insufficient work stability and liquid source pollution in the prior art. The device disclosed by the invention comprises a liquid adding device for adding reagents into a capillary tube body, wherein the liquid adding device comprises a funnel cup, an installation frame and liquid reagent cups; the bottom end of the funnel cup is communicated with the top end of the capillary tube body, the installation frame is provided with one or more tank body, and the liquid reagent cups for accommodating the reagents are arranged in the tan bodies; each liquid reagent cup comprises a cup body with an opening, a reagent arranged in the cup body and a sealing film arranged at the opening position of the cup body; the bottom end of each tank body is communicated with the top end of the funnel cup, and a puncturing device for puncturing the sealing film, when the liquid reagent cup downwards presses, to enable the reagent in the liquid reagent cup to flow into the funnel cup is further arranged at the bottom in each tank body. The capillary chemiluminiscence detection device disclosed by the invention has the advantages of reducing instrument cost, improving operation reliability and the like.

Description

technical field [0001] The invention belongs to the field of chemiluminescence detection, and in particular relates to a capillary chemiluminescence detection device and a detection method thereof. Background technique [0002] The disposable sampling capillary has the capillary phenomenon of automatically absorbing liquid, which can realize automatic liquid addition, but it is only effective for the first use. When the same capillary is used once and the capillary phenomenon is used to add liquid again, it will cause the liquid used later. source pollution. In this way, when the capillary is used as a disposable reaction container, it is impossible to carry out multi-step reactions such as immunoassays. During the immunoassay, two kinds of antibodies or antigens are used to combine the antigens or antibodies to be tested, and at the same time, washing liquid and Common reagents such as substrates are used to assist the experiment. If it is used as a fixed reaction vessel,...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: in the prior art, when a capillary is used as a disposable reaction container, multi-step reactions such as immune experiments cannot be carried out, while a capillary that can be used as an immune reaction container has high cost and poor work stability. Insufficient and liquid source pollution problems, the purpose is to provide a capillary chemiluminescence detection device and its detection method, which can not only effectively use the capillary as a disposable reaction container, reduce the amount of reagents, but also effectively solve the problem of reagent source pollution , the small volume of the capillary itself can also be used to realize the control of the reaction volume and reduce the demand for sample addition accuracy. The present invention not only reduces the cost of the instrument, but also improves the reliability of the operation

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