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Aeromonas salmonicida bacteriophage, bactericidal composition containing same and application thereof

A technology for killing Aeromonas salmon and bacteriophage, applied in the field of bactericidal compositions and killing Aeromonas salmon phage, can solve the problems of evolutionary phage, high dose, narrow host spectrum, etc.

Active Publication Date: 2020-08-25
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, as far as the current application of single phage therapy is concerned, some potential disadvantages gradually appear in the application process, such as a narrow range of host spectrum that can be lysed, a high dose required to lyse a certain number of hosts, and hosts evolving phage resistance in a short period of time. sex etc.

Method used

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  • Aeromonas salmonicida bacteriophage, bactericidal composition containing same and application thereof
  • Aeromonas salmonicida bacteriophage, bactericidal composition containing same and application thereof
  • Aeromonas salmonicida bacteriophage, bactericidal composition containing same and application thereof

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Embodiment

[0069] Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only for illustrating the present invention, and should not be considered as limiting the scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased from the market.

[0070] The invention discloses a phage cocktail preparation for efficiently cracking Aeromonas salmonicida atlantis. Using a strain of broad-spectrum drug-resistant pathogenic Aeromonas salmonicida isolated from the muscle, liver, and kidney of Atlantic salmon with skin ulceration as the host, 10 cleavage phage strains were isolated from the water enviro...

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Abstract

The invention relates to an aeromonas salmonicida phage, a bactericidal composition comprising the same and an application of the bactericidal composition. The phage is capable of quickly inhibiting growth of Atlantic aeromonas salmonicida within a short period of time and inhibiting generation of resistance of the pathogenic bacterium phage. In addition, the bactericidal composition comprising the phage is safe and effective, and is high in specificity and low in production cost, and the disadvantages of single phagotherapy are compensated in the control process of pathogenic bacterium.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to Aeromonas salmonicida bacteriophage, a bactericidal composition containing it and applications thereof. Background technique [0002] Aeromonas salmonicida ( Aeromonas salmonicida ) is a Gram-negative bacillus belonging to the genus Aeromonas under the phylum Proteobacteria. It is widely distributed in the water environment, especially in the intestinal tract of aquatic animals. bacteria. Aeromonas salmonicida has a broad host range and, in some cases, is responsible for a variety of infectious diseases in humans and animals, both in and out of the intestinal tract. Under high-density farming conditions, due to the high lethality and morbidity rate of Aeromonas salmonicida, this type of pathogenic bacteria is considered to be one of the main causes of explosive deaths of various aquatic animals. Trout losses were severe. In addition, this type of pathogenic bacteria can infect no...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K35/76A61P31/04C12R1/92
CPCA61K35/76C12N7/00C12N2795/00021C12N2795/00032Y02A50/30
Inventor 马迎飞陈玲袁盛建
Owner SHENZHEN INST OF ADVANCED TECH
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