39 results about "Phylum Proteobacteria" patented technology

According to the invention, through amplifying a strong promoter in Ensifer adhaerens and carrying out bioinformatics analysis and functional verification, a strong promoter is obtained, and the strong promoter can be widely used for gene expression, genetic manipulation and strain improvement in Sinorhizobium meliloti, Zymomonas mobilis, Caulobacter crescentus, Pseudomonas denitrificans, Agrobacterium tumefaciens, Brucella abortus, Pseudomonas fluorescens, Rhizobium leguminosarum, Ensifer adhaerens and other alpha-proteobacteria and has a nucleotide sequence of SEQ ID NO: 1. The invention also relates to a plasmid vector containing the strong promoter, a method for constructing a genetic engineering strain by using the strong promoter, and an application of the corresponding strain and ahost cell in starting expression of a target gene.

The invention discloses a method for quickly reducing antibiotics and resistance genes in organic solid waste, and belongs to the field of organic solid waste treatment. The method has the advantagesthat ultrahigh-temperature aerobic fermentation is carried out on the organic solid waste by the aid of aerobic zymocyte capable of tolerating the temperatures of at least 80 DEG C under the conditionthat pile temperatures are not lower than 80 DEG C under the control for at least 5-7 days, and accordingly effects of quickly and stably reducing the antibiotics and the resistance genes in the organic solid waste can be realized; the antibiotics and the ARGs (antibiotic resistance genes) can be quickly degraded by means of ultrahigh-temperature aerobic fermentation, integral microbial communitystructures in piles further can be changed, 90% of microorganisms (mainly including Proteobacteria and Bacteroidetes) which carry the ARGs can be killed, risks of gene transfer of the ARGs can be reduced, diffusion of the ARGs can be controlled from the source, and the ARGs can be guaranteed against rebounding; the organic solid waste can be treated, and double effects of efficiently removing antibiotic residues and resistance gene pollution can be realized; exogenous heating can be omitted, energy can be produced only by the aid of metabolism of thermophilic microorganisms to reach the highfermentation temperatures, and accordingly the method is low in energy consumption and is environmentally friendly.

The present invention provides a stable complex microbial system, which simultaneously decomposes a plurality of organic contaminants even under a polluted environment with these contaminants and permits more effective decomposition of persistent organic contaminants such as PCNB and simazine. A support for holding a complexed enrichment of degrading bacteria, which contains a porous material provided as a support on which degrading bacteria A capable of degrading at least one organic contaminant and degrading bacteria B capable of degrading another organic contaminant are enriched, is produced.

The degrading bacteria A may be a PCNB-degrading bacteria, particularly degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of Nocardioides sp. PD653 and the degrading bacteria B may be degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of β-Proteobacteria CDB21.

Screening assays that allow for the identification of agents that increase acyl homoserine lactone (AHL) acylase expression and/or AHL acylase activity in γ-proteobacteria such as Pseudomonas aeruginosa. Such agents are useful, for example, for inhibiting quorum sensing activity of such bacteria by increasing degradation of long chain, but not short chain, AHLs and, therefore, can be useful for treating infections by such bacteria.

The present invention provides a method for controlling crown gall disease in plants using an effective quantity of α-proteobacteria that produces trifolitoxin (TFX). The present invention also provides a biocontrol agent for use in the above method, and a plant coated with the biological control agent. The biocontrol agent is characterized as a biologically pure culture of an α-proteobacteria strain that produces TFX, or an α-proteobacteria strain genetically engineered to produce TFX. The α-proteobacteria strain employed may include any one of the many strains of Agrobacterium capable of producing crown galls, including Agrobacterium vitis and, in particular, A. vitis F2/5. The α-proteobacteria strain employed may be genetically engineered to produce TFX by introducing a genetic construct into the Agrobacterium so as to cause the Agrobacterium to carry and express the tfx operon from Rhizobium. The bacteria may also be genetically engineered to produce TFX by introducing a pT2TFXK plasmid into the Agrobacterium. The biocontrol agent may also be the strain Agrobacterium vitis F2/5 (pT2TFXK), ATCC Patent Deposit Designation PTA-2356.

The invention discloses Klebsiella pneumoniae, a bacterium agent of the Klebsiella pneumoniae and a preparation method and application. The Klebsiella pneumoniae FM84 is a thiram degradation bacterium; the preservation unit is China General Microbiological Culture Collection Center (CGMCC); the preservation date is December 15, 2015; the preservation number is CGMCC N0. 11663; the Klebsiella pneumoniae belongs to bacteria, proteobacteria, gamma-proteobacteria, enterobacteriales, enterobacteriaceae and Klebsiella. The bacterium agent with the Klebsiella pneumoniae as an active ingredient is a bacterium agent prepared by activating the Klebsiella pneumoniae FM84 through strains, culturing seeds, producing and culturing and preparing with a preparation. The preparation method comprises an activation culture step and a fermentation culture step; the invention provides application of the bacterium agent to preparation of agricultural plants capable of degrading thiram. The bacterium agent comprising the strains has a simple preparation process, is low in cost and convenient to use, and has very good application prospect.

The invention provides use of mussel polysaccharides and degradation products thereof for regulating intestinal microflora, and use of preparation of a food, a health food or a medicine for regulatingintestinal microflora of a human body and feed or a medicine for regulating intestinal microflora of animals. A food, a health food, feed and a medicine containing the mussel polysaccharides and thedegradation products thereof are provided. The mussel polysaccharides have a molecular weight ranging from 2 KDa to 400 KDa. The degradation product is mussel polysaccharides, the molecular weight range is 300 Da to 2 KDa, and the degree of polymerization is 2-10. The mussel polysaccharides serving as a prebiotic raw material are extracted from the marine animal mussel, the source of the raw material is rich, the production and preparation costs are low, the proportion of the phylum firmicutes of obese model rats can be significantly reduced, the proportion of bacteroides is improved, and themussel polysaccharides play a role of improving intestinal microecology and is a novel prebiotic.

The invention relates to a method for regulating microbial denitrifying florae on the basis of combination of powdered activated carbon and organic matter. According to the method, after activated anammox sludge, anaerobic granular sludge and activated carbon powder are added to an SAMBR, to-be-treated wastewater is introduced, the organic matter is added, and the microbial denitrifying florae areregulated. The method has the following advantages: microbial community structure in the anammox process can be domesticated effectively, and nitrogen-containing wastewater containing the organic matter can be removed from the domesticated activated sludge; proteobacteria becomes dominant phylum, relative content of predicted genes related to nitrite reductase, nitric oxide reductase and other enzymes throughout a denitrification pathway in a nitrogen circulation pathway is increased remarkably, the whole denitrification process is enhanced, and the anammox process can adapt to the wastewatercontaining the organic matter.

The invention relates to an illness state evaluating system and reagent kit for systemic lupus erythematosus, and belongs to the technical field of biomedicine detection. An evaluating system is provided for conveniently and swiftly evaluating the illness state of the systemic lupus erythematosus. The evaluating system comprises the steps of calculating the ratio of proteobacteria/bacteroidetes (Proteobacteria/Bacteroidetes,P/B) of intestinal tracts of a patient, comparing the ratio with the reference value of healthy crowds (the medical reference value range set based on the early-stage research result is 0.13 to 0.63), when the ratio is higher than the reference value of the healthy crowds, performing effective assistance on SLE disease evaluation; and when the ratio is increased along with increase of an SLE disease activity index (SLE disease activity index, SLEDAI) mark, performing effective evaluation on SLE disease activity according to changes of the ratio. In addition, the invention further provides a corresponding reagent kit.

The invention relates to a method for fast and quantitatively measuring the microbial community composition in the fermentation process of baijiu. The method is characterized in that phylum specific primers are used in a PCR system so that special amplification of microbial communities of a certain specific phylum can be performed in the fermentation process of baijiu, quantitative amplification is performed through a qPCR system, and quantitative recognition is achieved. According to the category of known microorganisms, primers of the bacteroidetes, proteobacteria, firmicutes, synergistetes and actinobacteria are respectively designed, and the microstructure change of microbial communities can be fast inspected in the baijiu fermentation process in the level of the phylum on the basis of quantitative amplification of the phylum specific primers.

The invention belongs to the technical field of molecular biology, and provides a biomembrane-alpha proteobacteria quantitative determination method. The method comprises the following steps: making a sample of alpha-proteobacteria, recovering the sample of alpha-proteobacteria, preparing Escherichia coli competent cell, conversing the alpha-proteobacteria sample to competence bacteria; culturing by selecting bacterium of the sample of alpha-proteobacteria, extracting the plasmid of the sample of alpha-proteobacteria, determining the quantification double chain DNA content of alpha-proteobacteria, pretreating the biomembrane sample to be measured; and performing real-time fluorescence quantification PCR detection. The method has the advantages of fast analysis speed, less restriction factor, high accuracy, and reduced subjective factor influence, and provides support for microbe stability of a drinking water system as well as scientific basis for purifying water quality of a feed pipe net.

The invention discloses a strong promoter from ensifer adhaerens as well as a plasmid vector and an application of the strong promoter. Function verification is performed by amplifying one strong promoter in ensifer adhaerens, and the strong promoter which can be widely applied to gene expression, gene operation and strain improvement of alpha-proteobacteria such as sinorhizobium meliloti, zymomonas mobilis, caulobacter crescentus, pseudomonas denitrificans, agrobacterium tumefaciens, brucella abortus, pseudomonas fluorescens, rhizobium leguminosarum and ensifer adhaerens is obtained and has the nucleotide sequence being SEQ ID NO:1. The invention also relates to the plasmid vector containing the strong promoter, a method for constructing a gene engineering strain by use of the promoter, the corresponding strain and an application of a host cell in starting target gene expression.

The invention provides a gene chip for detecting beta-proteobacteria communities in a marine environment. The gene chip comprises a chip carrier and a probe which is fixed on the chip carrier and used for detecting Alcaligenaceae bacteria, Burkholderiaceae bacteria, Comamonadaceae bacteria, Hydrogenophilaceae bacteria, Methylophilaceae bacteria, Neisseriaceae bacteria, Nitrosomonadaceae bacteria, Oxalobacteraceae bacteria and Rhodocyclaceae bacteria in seawater. The probe used for the chip can realize rapid and high-throughput detection of beta-proteobacteria communities in seawater, and can detect up to 9 beta-proteobacteria families' information. Therefore, the invention provides powerful technical support for monitoring bacterial communities in the marine environment and has laid a solid foundation for establishing and improving an offshore and marine environmental quality comprehensive assessment system.

The invention discloses a xylose-induced promoter. By amplifying a xylose-induced promoter in sinorhizobium meliloti, bioinformatics analysis and function verification are carried out, the xylose-induced promoter which can be widely applied to gene expression, genetic gene operation and strain improvement in alpha-proteobacteria such as Sinorhizobium meliloti, Zymomonas mobilis, Caulobacter crescentus, Pseudomonas denitrificans, Agrobacterium tumefaciens, Brucella abortus, Pseudomonas fluorescens, Rhizobium leguminosarum and Sinorhizobium adhaerens is obtained, and the nucleotide sequence of the promoter is SEQ ID NO: 1 or SEQ ID NO: 2. The invention also relates to a plasmid vector containing the xylose-induced promoter, a method for constructing a genetic engineering strain by using thepromoter, a corresponding strain, and application of a host cell to induction and starting of expression of a target gene under xylose inducible conditions.

The invention discloses a production process of novel chicken soup-stock. The production process comprises the following steps: raw material inspection, raw material storage, raw material CCP1, unfreezing, cooking, filtering, CCP2 mixed sterilization, inner packaging, sterilization or no sterilization, labeling, quick freezing, CCP3 gold detection, outer packaging, warehousing, qualified inspection and delivery. According to the method, the optimal process is achieved in the indexes such as nutrition, taste, color and fragrance, meanwhile, the indexes of other pathogenic bacteria such as the total number of bacterial colonies, escherichia coli, streptococcus, salmonella, staphylococcus and pseudomonas aeruginosa are remarkably reduced or not detected, and the optimal hygienic index and the process standard of the sterilization effect are achieved; moreover, in the whole chicken soup-stock processing process, workshop standardized production processing is adopted, the taste is stable, the nutritional value is better guaranteed, inconvenience caused by household making of existing traditional clear chicken soup is avoided, the production efficiency is improved, and the wide requirements of existing marketization are greatly met.

The invention relates to a biological reduction technology for composite pollutants, and aims to provide a method for reducing hexavalent chromium in a methane matrix biomembrane by NO<3><->. The method includes the following steps: adding Na2CrO4 and NaNO3 to an inorganic medium, and allowing the obtained medium, for later use, to stand under a micro-aerobic condition; introducing simulated wastewater into a methane matrix membrane bioreactor, adding CrO<4><2-> and activated sludge, and carrying out self-circulation to obtain the a bacterial solution; introducing the simulated wastewater andCH4 into the reactor in a continuous flow manner, and controlling the concentration of NO<3><-> in inlet water in stages; and ensuring that the concentrations of the CrO<4><2-> and NO<3><-> in outletwater reach a steady state for at least 10 days in every running stage. Compared with the prior art, the method has the following advantages: the high load of NO<3><-> has a significant irreversible inhibition effect on the reduction of Cr(VI); the introduction of the NO<3><-> deceases Meiothermus originally involved in the reduction of Cr(VI) and makes alpha-proteobacteria enriched; and when theNO<3><-> is removed, beta-proteobacteria become important, so the beta-proteobacteria play a potential role in Cr(VI) reduction.