Method for quickly reducing antibiotics and resistance genes in organic solid waste

A technology of organic solids and resistance genes, applied in the removal of solid waste, microorganism-based methods, preparation of organic fertilizers, etc., can solve the problems of ARGs and antibiotics contamination, increase of ARGs content, lack of efficient and economical treatment technology, etc.

Active Publication Date: 2018-05-15
FUJIAN AGRI & FORESTRY UNIV
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  • Application Information

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Problems solved by technology

Xie et al. reported that after long-term application of organic fertilizers made from traditional sludge composting, the content of ARGs in farmland soil increased significantly, and there was a risk of expanding pollution and spreading
So far, there is still a lack of efficient and economical treatment technology, which can not only solve the p...

Method used

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  • Method for quickly reducing antibiotics and resistance genes in organic solid waste
  • Method for quickly reducing antibiotics and resistance genes in organic solid waste
  • Method for quickly reducing antibiotics and resistance genes in organic solid waste

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Embodiment 1

[0054] 1) The configuration of the ultra-high temperature fermentation liquid bacterial agent: hyperthermophilic bacteria Calditerricola yamamurae UTM801, thermophilic bacteria Thermus thermophilus FAFU013, thermophilic Bacillus geobacillussp.UTM801, methylotrophic Bacillus Bacillus methylotrophicus UTM401, Bacillus sp.UTM03 was respectively activated and cultivated until the logarithmic growth phase, and the bacteria were mixed evenly according to the mass ratio of 1:1:1:1:1 for later use.

[0055] 2) Material blending: Mix sludge (moisture content 80%) and auxiliary material rice husk (moisture content 15%) according to the volume ratio (1:4). At this time, the moisture content of the mixed material is about 55%, and add 0.05% The ultra-high temperature aerobic fermentation bacterial agent, mash some agglomerates with a diameter greater than 10cm, and stir evenly.

[0056] 3) Ultra-high temperature aerobic composting: stack the mixed materials into the fermentation tank for ...

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Abstract

The invention discloses a method for quickly reducing antibiotics and resistance genes in organic solid waste, and belongs to the field of organic solid waste treatment. The method has the advantagesthat ultrahigh-temperature aerobic fermentation is carried out on the organic solid waste by the aid of aerobic zymocyte capable of tolerating the temperatures of at least 80 DEG C under the conditionthat pile temperatures are not lower than 80 DEG C under the control for at least 5-7 days, and accordingly effects of quickly and stably reducing the antibiotics and the resistance genes in the organic solid waste can be realized; the antibiotics and the ARGs (antibiotic resistance genes) can be quickly degraded by means of ultrahigh-temperature aerobic fermentation, integral microbial communitystructures in piles further can be changed, 90% of microorganisms (mainly including Proteobacteria and Bacteroidetes) which carry the ARGs can be killed, risks of gene transfer of the ARGs can be reduced, diffusion of the ARGs can be controlled from the source, and the ARGs can be guaranteed against rebounding; the organic solid waste can be treated, and double effects of efficiently removing antibiotic residues and resistance gene pollution can be realized; exogenous heating can be omitted, energy can be produced only by the aid of metabolism of thermophilic microorganisms to reach the highfermentation temperatures, and accordingly the method is low in energy consumption and is environmentally friendly.

Description

technical field [0001] The invention belongs to the field of organic solid waste treatment, in particular to a method for rapidly reducing antibiotics and resistance genes in sludge. Background technique [0002] my country is the largest producer and consumer of antibiotics in the world. In 2013, the total amount of antibiotics used in my country reached 162,000 tons, half of which was used in livestock and poultry breeding. In order to prevent the infection of livestock and poultry, farmers often feed large doses of antibiotics, but most of these antibiotics are not absorbed, and about 30-90% of them are excreted with feces and urine in the form of original drugs or metabolites, resulting in livestock and poultry feces Antibiotic residues exceeded the standard. The irrational use and long-term abuse of antibiotics have resulted in excessive antibiotic residues in the environment, resulting in increased selection pressure for drug-resistant microorganisms in the environmen...

Claims

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Application Information

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IPC IPC(8): C05F17/00C12N1/20C12R1/07C12R1/01
CPCC12N1/20C05F17/50C05F17/20Y02W30/40B09B5/00C12R2001/07C12N1/205C12R2001/01B09B3/40B09B3/00
Inventor 周顺桂廖汉鹏陈志
Owner FUJIAN AGRI & FORESTRY UNIV
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