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Paracoccus and its bacterial agent, preparation method and application

A kind of paracoccus and bacterial agent technology, applied in the field of microorganisms, to achieve the effect of good potential application value

Active Publication Date: 2017-11-17
YUNNAN ACAD OF TOBACCO AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And a kind of microbial agent that has multiple effects has no relevant reports

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  • Paracoccus and its bacterial agent, preparation method and application
  • Paracoccus and its bacterial agent, preparation method and application
  • Paracoccus and its bacterial agent, preparation method and application

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preparation example Construction

[0033] Paracoccus of the present invention is the preparation method of bacterial agent of active ingredient, comprises plate culture, seed culture, fermentation, bacterial agent preparation steps, specifically comprises:

[0034] A. Plate culture: inoculate Paracoccus limosus A36 on the plate medium, and culture it at 26-30°C for 48-54 hours to obtain plate strains;

[0035] B. Seed culture: inoculate the plate strains into the liquid medium, and cultivate them on a shaker at a temperature of 26-30°C for 55-65 hours to obtain liquid-fermented seeds;

[0036] C. Fermentation: Inoculate the liquid fermented seeds into the fermenter according to the volume of 8~12% of the fermentation medium volume, cultivate at 28~32°C for 36~54h, the stirring speed is 170~190 r / min, and the ventilation volume is 60~100 %, to obtain Paracoccus (Paracoccuslimosus) A36 fermentation broth;

[0037] D. Bacterial agent preparation: add an adsorbent to the fermentation broth for adsorption and air-d...

Embodiment 1

[0047] ——Preparation of preparations containing Paracoccus limosus A36 as active ingredient

[0048] Take Paracoccus limosus cultured on the NA plate and inoculate it into a 250ml Erlenmeyer flask filled with NB liquid culture solution, cultivate it on a shaker with a rotation speed of 100-110 rpm for 60 hours, and then use 10% of the inoculum amount Then transfer it to a tissue culture orchid vase with liquid culture medium, cultivate it on a shaking table with a rotation speed of 100-110 rpm for 72 hours, and then use 80L of peat with a particle size of 1-5mm for adsorption to form a solid bacterial formulation .

[0049] The above-mentioned NA medium components are: peptone 10.0g, beef powder 5.0g, sodium chloride 5.0g, agar powder 15.0g, distilled water 1000m, pH7.0-7.5. The composition of NB culture medium is: peptone 10.0g, beef powder 5.0g, sodium chloride 5.0g, distilled water 1000m, pH7.0-7.5.

[0050] The above cultivation temperature is room temperature 20-25° C.,...

Embodiment 2

[0052] ——Preparation of preparations containing Paracoccus limosus A36 as active ingredient

[0053] Take the Paracoccus limosus cultured on the NA plate and inoculate it into a tissue culture orchid vase with NB liquid culture medium, cultivate it on a shaker with a rotation speed of 100-110 rpm for 60 hours, and inoculate at 10% The amount is then transferred to the CT5-2 automatic fermentation tank equipped with liquid fermentation culture medium, the feeding amount is 3.5L, the fermentation culture time is 48 hours, and 80L of peat with a particle size of 1-5mm is used for adsorption to form solid bacteria dosage form.

[0054] The above-mentioned NA medium components are: peptone 10.0g, beef powder 5.0g, sodium chloride 5.0g, agar powder 15.0g, distilled water 1000m, pH7.0-7.5. The composition of NB culture medium is: peptone 10.0g, beef powder 5.0g, sodium chloride 5.0g, distilled water 1000m, pH7.0-7.5.

[0055] The ventilation volume of the sterile air in the CT5-2 a...

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Abstract

The invention discloses a paracoccus and its preparation, preparation method and application. The paracoccus is Paracoccus limosus (Paracoccus limosus) A36. Date: November 21, 2014, deposit number: CGMCC No. 10032; the Paracoccus belongs to the kingdom Bacteria, the phylum Proteobacteria, the class α-Proteobacteria, the order Rhodobacteria, the family Rhodobacteraceae, and the genus Paracoccus. The bacterial agent in which the paracoccus is the active ingredient is a bacterial agent made from the paracoccus actinomycetes through plate culture, seed culture, and fermentation; the preparation method of the bacterial agent in which the bacillus is the active ingredient includes a flat plate Cultivation, seed cultivation, fermentation, and bacterial agent preparation steps; the application of the bacterial agent in preparing phosphorus-dissolving, potassium-relieving, and improving soil quick-acting nitrogen-based fertilizers.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a paracoccus and its bacterial agent, preparation method and application. Background technique [0002] With the extensive application of chemical fertilizers in modern agricultural production, the output value and yield of crops have been greatly improved, but it has also led to a series of problems such as the reduction of soil organic matter, soil compaction, and land productivity decline. Soil microorganisms can promote the transformation of soil nutrients, activate soil nutrients, and increase nutrient supply. Soil microorganisms participate in various biochemical reactions of soil nutrient transformation, which are closely related to the supply and loss of soil nutrients. In soil, soil microorganisms are involved in processes such as mineralization and humification of organic matter, nitrification and denitrification of nitrogen, and effective use of pho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05G3/00C12R1/01
Inventor 段玉琪夏振远杨宇虹徐照丽张立猛张翠萍
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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