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The use of sodium copper chlorophyllin

A technology of sodium copper chlorophyllin and bacteria, which is applied in the field of medicine to achieve the effect of alleviating liver disease and increasing the number of bacteria

Active Publication Date: 2017-11-07
CHENGDU TONGDE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no mechanism study on the restoration of intestinal microecological balance with sodium copper chlorophyllin as the active ingredient, or drugs containing sodium copper chlorophyllin

Method used

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  • The use of sodium copper chlorophyllin
  • The use of sodium copper chlorophyllin
  • The use of sodium copper chlorophyllin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: sodium copper chlorophyllin protects the small intestinal chorion

[0040] 1. Materials and methods

[0041] 1.1 Materials

[0042] Male C57BL / 6 and BALB / C mice were provided by Beijing Huafukang Biotechnology Co., Ltd.; chlorophyllin sodium copper powder was provided by Chengdu Tongde Pharmaceutical Co., Ltd., and the molecular formula is C 34 h 29 CuN 4 Na 3 o 6 , molecular weight: 722.13, CAS number: 65963-40-8; carbon tetrachloride (CCl 4 ) and mineral oil were provided by sigma; methionine choline deficiency (MCD) feed was provided by Nantong Trofee Feed Technology Co., Ltd.

[0043] 1.2 Reagent preparation

[0044] Sodium copper chlorophyllin agent (mother solution): Add 50 ml of sterilized ultrapure water and 100 mg of sodium copper chlorophyllin powder into a 50 ml sterilized centrifuge tube in turn in an ultra-clean workbench, vortex to mix and store at 4°C.

[0045] CCl 4 Injection: 0.75 ml, 2.25 ml, 3.75 ml of CCl were respectively mixed ...

Embodiment 2

[0056] Example 2: Oral administration of sodium copper chlorophyllin can effectively prevent liver fibrosis / cirrhosis

[0057] 1.1 Materials

[0058] Male BALB / C mice were provided by Beijing Huafukang Biotechnology Co., Ltd.; sodium copper chlorophyllin powder was provided by Chengdu Tongde Pharmaceutical Co., Ltd., and the molecular formula is C 34 h 29 CuN 4 Na 3 o 6 , molecular weight: 722.13, CAS number: 65963-40-8; carbon tetrachloride (CCl 4 ) and mineral oil are provided by sigma;

[0059] 1.2 Reagent preparation

[0060] Sodium copper chlorophyllin agent (mother solution): Add 50 ml of sterilized ultrapure water and 100 mg of sodium copper chlorophyllin powder into a 50 ml sterilized centrifuge tube in turn in an ultra-clean workbench, vortex to mix and store at 4°C.

[0061] CCl 4 Injection: 0.75 ml, 2.25 ml, 3.75 ml of CCl were respectively mixed in the ultra-clean workbench 4 and 14.25ml, 12.75ml, 11.25ml of mineral oil were added to a 15ml sterilized ce...

Embodiment 3

[0068] Example 3: Sodium copper chlorophyllin up-regulates Firmicutes bacteria in the intestinal tract of animals with liver cirrhosis

[0069] image 3 Shown are the results of relative quantification of fecal bacteria in mice. Compared with normal healthy mice, the relative content of Firmicutes bacteria in the feces of mice with liver cirrhosis induced by carbon tetrachloride injection was significantly reduced, and the bacteria of Firmicutes in mice treated with sodium copper chlorophyllin returned to normal levels . As Gram-positive cells, Firmicutes are the most dominant commensal bacteria in the gut. It is generally believed that Firmicutes decompose undigested food in the small intestine in the intestine, produce short-chain fatty acids, and obtain energy absorption. We found that long-term injection of carbon tetrachloride caused liver cirrhosis, and we found that the process of liver cirrhosis was related to the decline of intestinal Firmicutes bacteria. A large ...

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Abstract

The invention discloses a new application of sodium copper chlorophyllin, relates to the field of medicine, and relates to the application of sodium copper chlorophyllin in the preparation of medicines for treating and regulating intestinal flora. The beneficial effects of the present invention are: the sodium copper chlorophyllin is similar to prebiotics, which balances the microbial flora ecology of the intestinal tract, thereby limiting the growth of conditional pathogenic bacteria and the release of bacterial toxins in the intestinal tract, thereby alleviating liver disease. The present invention verifies through experiments that the sodium copper chlorophyllin can be used to regulate intestinal flora, increase the number of Firmicutes bacteria, and inhibit the abundance of α-proteobacteria and γ-proteobacteria. The imbalance of intestinal flora is closely related to various liver diseases, including liver failure and cirrhosis. Therefore, the preparation of sodium copper chlorophyllin can be used to prevent and treat various liver diseases.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of sodium copper chlorophyllin. Background technique [0002] Relationship between dysbiosis of gut microbiota and fatty liver, obesity, and metabolic syndrome. Intestinal microecology (microbiota) is an inseparable part of the normal human body. It participates in the establishment of the intestinal immune system, produces vitamin B and vitamin K, helps break down food, and assists the body to resist the invasion of pathogenic bacteria. The number of symbiotic microorganisms in the human body is 10 times the number of our human cells (100 trillion), most of which are anaerobic bacteria and cannot be cultured in vitro. Bacterial cells in the human gut can number up to 10 14 , accounting for 78% of the total microbial biomass in the human body. There are about 400 to 900 species of intestinal bacteria. There are also other microorganisms in the human intestinal tract, i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/409A61P1/00A61P1/16
Inventor 韩源平郑翰范伟川
Owner CHENGDU TONGDE PHARMA
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