Hyperspectral microscopy imaging and polarization modulation apparatus based on LCTF and method thereof

Abstract

The invention provides a hyperspectral microscopy imaging and polarization modulation apparatus based on LCTF and a method thereof. The hyperspectral microscopy imaging and polarization modulation apparatus is an integration system by combing a microscopy imaging technology and a hyperspectral imaging and polarization modulation technology. The apparatus comprises a microscopic imaging module, a spectrum splitting module, a polarization modulation module, and an image processing module. The method is characterized in that broadband light source is subjected to light splitting by LCTF, light with different center wavelengths can be output, the specific angle rotation is carried out on a polarizing film, the polarization modulation is carried out on the light from a sample, the image is taken once when a camera is rotated for one time, three-time rotation is taken as a single wave band period, the collected light intensity graph with different polarization information is input into a computer for being processed, and a polarization data cube of the sample under single wavelength is obtained, then a next center wavelength is output by LCTF, the rotation of the polarizing film is repeated, the image taking by the camera is repeated until sampling at each wave band is completed, and a polarization spectrum data set of the sample is obtained. The method has the advantages of simple optical path, low cost, and comprehensive measure information.

Description

A device and method for hyperspectral microscopic imaging and polarization modulation based on LCTF technical field The invention belongs to the technical field of micro-spectral imaging, and in particular relates to a micro-imaging device and method for spectrally splitting light through liquid crystals combined with polarization modulation. Background technique Since the 1860s, spectroscopy has been used for qualitative and quantitative analysis of substances. After more than a hundred years of development, spectral analysis technology has gradually matured and been used in various fields. However, it was not until the 1980s that spectral technology was combined with two-dimensional imaging technology to produce spectral imaging technology, that is, map-spectrum integration. This technology is mainly used in the field of remote sensing, and is gradually expanding to application fields such as agriculture, minerals, archaeology, and microscopic imaging. Due to the diffe...

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Problems solved by technology

The spectral image obtained by filter wheel filtering is relatively scattered, and only certain imaging under certain specific spectra can be obtained, but its complete and wide range of spectral characteristics cannot be obtained.

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