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Construction of rat alveolar macrophage cell line for expressing porcine CD163 molecule and application thereof

A technology of alveolar macrophages and MH-SCD163, applied in the field of biology, can solve problems such as limited research, inability to guarantee stability, and inability to evaluate immune indicators, and achieve the effect of expanding the scope of research

Inactive Publication Date: 2017-09-01
NORTHWEST A & F UNIV
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Problems solved by technology

Because primary PAM cells need to be obtained from the lungs of dissected pigs, the cost of obtaining PAM is high, and the stability between different batches cannot be guaranteed; since Marc-145 cells are derived from African green monkeys and belong to renal epithelial cells, Non-macrophages, the relevant immune indicators after virus infection cannot be evaluated, so the in vitro research of PRRSV is limited. It is necessary to establish a cell line that can replace PAM for the isolation, culture and evaluation of relevant immune indicators after infection of PRRSV

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  • Construction of rat alveolar macrophage cell line for expressing porcine CD163 molecule and application thereof
  • Construction of rat alveolar macrophage cell line for expressing porcine CD163 molecule and application thereof
  • Construction of rat alveolar macrophage cell line for expressing porcine CD163 molecule and application thereof

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[0021] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0022] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0023] The mouse alveolar macrophage cell line expressing porcine CD163 molecule provided by the embodiment of the present invention is named MH-S CD163 , its collection number is: CCTCC NO: C2016167, received by the China Center for Type Culture Collection on October 19, 2016, and registered in the register, and preserved for 30 years from October 19, 2016; Tested on October 28, 2011, the result was survival; proposed classification and naming: mouse alveolar macrophage cell line MH-...

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Abstract

The invention belongs to the technical field of biology, and discloses construction of a rat alveolar macrophage cell line for expressing porcine CD163 molecule and application thereof. The rat alveolar macrophage cell line is MH-SCD163, and the preservation number is CCTCC NO: C2016167. The virus titer of PRRSV infection can be greater than 105TCID50 / mL. A constructed MH-S cell line for stably expressing porcine CD163 can be applied to the separation and culture of PRRSV virus, and can be further applied to the research of PRRSV cell receptors. The construction of the PRRSV impressionable rat alveolar macrophage cell line provides an alternative cell line for the culture of PRRSV, and can be applied to the separation, culture and multiplication of PRRSV and the research of the immunologic mechanism of PRRSV.

Description

technical field [0001] The invention belongs to the technical field of biology, and in particular relates to the establishment and application of a mouse alveolar macrophage cell line expressing porcine CD163 molecule. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS) is an acute infectious disease of pigs caused by PRRS virus (PRRSV) infection, which causes huge economic losses to the pig industry. Because PRRSV has genetic variability, cytotropism, and its pathogenic mechanism is still unclear, the cell lines currently used to isolate, culture and study PRRSV are mainly porcine alveolar macrophages (PAM) and African green monkey kidney cells (Marc -145). Studies have shown that the cellular tropism of PRRSV is determined by the viral receptors on the surface of host cells. The current study found that the porcine CD163 molecule is a major cell receptor of PRRSV, which can mediate PRRSV non-susceptible cell lines into PRRSV-susceptible cel...

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Application Information

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IPC IPC(8): C12N5/10C12N15/867C12N7/00C07K14/705C12R1/91
CPCC07K14/70596C12N5/0688C12N7/00C12N2770/10021C12N2770/10071
Inventor 周恩民李亮亮
Owner NORTHWEST A & F UNIV
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