Method for detecting parasitism rate and ovipositing quantity of aphelinus mali in eriosoma lanigerum

Abstract

The invention provides a method for detecting parasitism rate and ovipositing quantity of aphelinus mali in eriosoma lanigerum. An invented primer pair is used for conducting PCR amplification, the aphelinus mali and target genes of the aphelinus mali in genomic DNA of the eriosoma lanigerum can be detected, and accordingly, whether the eriosoma lanigerum is parasitized by the aphelinus mali or not is distinguished. By adoption of the method provided by the invention, the ovipositing quantity of the aphelinus mali can be quite clearly calculated, and calculation is more accurate and easier than an anatomical method; the parasitism rate of the parasites in the eriosoma lanigerum can be accurately calculated, the pest control effect of the aphelinus mali on the eriosoma lanigerum is confirmed, and the phenomenon that certain aphelinus mali eggs die in the development process of the aphelinus mali or part of the eriosoma lanigerums parasitized by the aphelinus mali fall preys or are lost, and consequentially the parasitism rate is low is avoided.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology detection, and in particular relates to a method for detecting the parasitism rate and egg laying rate of the apple cotton aphid by sun bees. Background technique [0002] The apple aphid Eriosoma lanigerum (Hausmann) is an important pest of the genus Apple. It has strong reproductive ability. Because it has a layer of cotton wool on its body, it will cause little damage when spraying liquid medicine. Ordinary chemicals cannot kill it at all. it. In recent years, the harm in our country has become increasingly serious, and there is a tendency to further expand and spread. The apple aphid mainly parasitizes the adults and nymphs on apple trees throughout the year, and often forms tumor-like galls on the branches and roots of apples, directly affecting the growth and development of apple trees, the differentiation of flower buds, and the deterioration of fruit quality; Gradually widening,...

AI Technical Summary

Problems solved by technology

[0004] The sun bee achieves parasitic effect on the apple aphid by laying eggs in the apple cotton aphid. The eggs develop into larvae in the apple cotton aphid and absorb their nutrients. Before and after the sun bee pupates, the apple aphid turns black and dies. In the past, the statistics of the parasitism rate of the sun bee were based on the blackening and death of the apple aphid, and the number of apple aphids parasitized by the sun bee was counted. After the sun bee parasitized the apple cotton aphid in the field, some of the apple aphids were parasitized by the sun bee during the development process of the sun bee. The apple cotton aphid parasitized by the sun bee will be lost due to the predation of other natural enemies and the erosion of rainwater. More importantly, in the field, not all eggs of the sun bee can develop to the larval or pupal stage, and some apple cotton aphids parasitized by the sun bee For aphids, the eggs of the sunbee cannot hatch or die before they reach the pupal stage. Therefore, it is not accurate to count the number of blackened and dead apple aphids as the number of sunbee parasitized apple aphids, and the calculated parasitism rate is also inaccurate. precise

In the past, the statistical method of egg laying by sun bee was usually dissected. Under the dissecting microscope, the apple aphid was dissected to observe whether there were sun bee eggs in the body of the apple cotton aphid. However, the eggs of the sun bee were oblong, pearly white, and The body fluid of the apple aphid is similar, and it is extremely small, and it is difficult to distinguish it even under a dissecting microscope. This method is not accurate enough to count the eggs laid by the sunbee, and it is time-consuming and labor-intensive

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