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Yak skin collagen sponge having rapid haemostasis effect, and production method and application thereof

A technology of skin collagen and hemostatic effect, which is applied in the field of biomedical materials, can solve the problem of single variety, etc., and achieve the effect of wide application, obvious effect and good flexibility

Pending Publication Date: 2017-09-05
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing hemostatic collagen sponges mostly use bovine Achilles tendon as raw material to extract collagen or gelatin, and then combine other high molecular biomaterials, high molecular polymers or preparation technologies to prepare collagen sponges. The variety is single, so the development effect is better The variety of hemostatic collagen sponges is of great significance

Method used

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  • Yak skin collagen sponge having rapid haemostasis effect, and production method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Preparation of Yak Skin Collagen Sponge

[0039] (1) Clean the yak skin of the fresh Qinghai-Tibet plateau yak Wild Yak, soak it in 5% NaOH solution for 48 hours, remove the yak hair, wash it with distilled water for 3 times until neutral, and cut it into small pieces with a length of 5-7 cm and a width of 5-7 cm , and remove the epidermis and subcutaneous tissue layers to obtain the dermis;

[0040] (2) Preparation of type I collagen: the yak skin dermis pieces obtained in step (1) are further chopped, and the length is 1-2 mm, and the width is 1-2 mm. 0.5mol / L acetic acid solution, the ratio of solid to liquid is 1:15, and the weight ratio of yak hide:pepsin is 100:1.0. Stir and extract at low temperature for 16 hours, after filtration, add NaCl to the filtrate for salting out, the final concentration of NaCl is 4mol / L, stir for 10h, centrifuge at low temperature, dissolve the precipitate in 0.5mol / L glacial acetic acid, and then use 2.5, 1.8 and 0.9mol / L NaCl wa...

Embodiment 2

[0047] 1. Preparation of Yak Skin Collagen Sponge

[0048] (1) Clean the yak hide of the fresh Qinghai-Tibet Plateau yak Wild Yak, soak it in 10% NaOH solution for 12 hours, remove the yak hair, wash it with distilled water for 5 times until neutral, and cut it into small pieces with a length of 6-8 cm and a width of 6-8 cm , and remove the epidermis and subcutaneous tissue layers to obtain the dermis;

[0049] (2) Preparation of type I collagen: the yak skin dermis pieces obtained in the step (1) are further chopped, and the length is 2-3 mm, and the width is 2-3 mm. 0.5mol L-1 acetic acid solution, the ratio of solid to liquid is 1:20, and the weight ratio of yak hide:pepsin is 100:1.5. Stir and extract at low temperature for 24 hours. After filtering, add NaCl to the filtrate for salting out. The final concentration of NaCl is 5 mol / L, stir for 12 hours, and centrifuge at low temperature. 1.0mol / L NaCl was repeatedly salted out and acid-dissolved to obtain a crude collage...

Embodiment 3

[0056] 1. Preparation of Yak Skin Collagen Sponge

[0057] (1) Clean the yak hide of fresh Qinghai-Tibet Plateau yak Wild Yak, soak it in 8% NaOH solution for 36 hours, remove the yak hair, wash it with distilled water for 4 times until it becomes neutral, and cut it into small pieces with a length of 5-6 cm and a width of 5-6 cm , and remove the epidermis and subcutaneous tissue layers to obtain the dermis;

[0058] (2) Preparation of type I collagen: the yak skin dermis pieces obtained in step (1) are further chopped, and the length is 1-2 mm, and the width is 1-2 mm. 0.5mol / L acetic acid solution, the ratio of solid to liquid is 1:30, and the weight ratio of yak hide:pepsin is 100:2. Stir and extract at low temperature for 24 hours, after filtration, add NaCl to the filtrate for salting out, the final concentration of NaCl is 3mol / L, stir for 10h, centrifuge at low temperature, dissolve the precipitate in 0.5mol / L glacial acetic acid, and then use 2.0, 1.4 and 0.9mol / L Na...

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Abstract

The invention discloses a yak skin collagen sponge having a rapid haemostasis effect, and a production method and an application thereof. The rapid haemostasis collagen sponge is a scaffold having a three-dimensional porous structure, obtained through freeze drying or direct drying of a yak skin extract I collagen and gelatin mixed solution. The rapid haemostasis collagen sponge can accelerate the haemostasis speed of wounds, promote the proliferation of wound cells, prevent adhesion and accelerate the healing of the wounds, and can be used as a filler. The yak skin collagen sponge has the advantages of good endophilicity, realization of uniform and close adherence to the wounds, promotion of the healing of the wounds, certain mechanical strength, softness, no deformation, and good biocompatibility. The rapid haemostasis collagen sponge has wide uses, can be applied to the haemostasis of epidermal hemorrhage and the haemostasis of excessive hemorrhage of internal organ tissues, and also can be applied to the neurosurgery department, the orthopaedics, the gynaecology, the general surgery, operating rooms and other fields in clinic medical science.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a yak skin collagen sponge with rapid hemostasis and its preparation method and application. Background technique [0002] Collagen is the main component of the extracellular matrix, distributed in various organs of the body, and is the most abundant protein in the body, accounting for about 25% to 30% of the total protein in the body. Collagen contains a large number of polar groups, has surface activity and good compatibility. The unique triple helix structure of collagen endows it with good biocompatibility, biodegradability, low antigenicity and other characteristics. In addition, collagen molecules can activate the endogenous blood coagulation pathway, and the hydroxyproline contained in it is a repairing amino acid. When collagen interacts with platelets, it can quickly cause a series of subsequent processes associated with blood aggregation. At the same ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/00
CPCA61L24/102A61L24/0015A61L24/0036A61L24/0042A61L24/104A61L2300/252A61L2300/412A61L2300/418A61L2300/604A61L2400/04
Inventor 杨红霞李岑肖远灿毕宏涛高婷婷杜玉枝魏立新
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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