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Method for producing enzymatically modified stevioside with total glycoside content of 80% by using active-enzyme deep reaction

A production method and technology of stevioside, which is applied in the field of stevioside, can solve the problems of low conversion rate and limit the application of stevioside, and achieve the effects of increasing conversion rate, reducing production cost and improving sweetness quality

Inactive Publication Date: 2017-09-05
安徽润海生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Stevioside has no toxic side effects, no carcinogens, and is safe to eat. Regular consumption can prevent hypertension, diabetes, obesity and other diseases. It is an ideal sweetener to replace sucrose, but the existing production methods of stevioside have defects. If the conversion rate is low, and the extracted stevioside has a strong after-bitter taste, the application of stevioside is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A production method for obtaining 80% enzyme-modified stevioside of total glycosides through active enzyme deep reaction, comprising the following steps:

[0020] S1: Solution preparation, take a certain amount of pure water and starch into the tank, seal the opening of the tank through the sealing film, mix and stir to prepare a certain concentration of starch solution;

[0021] S2: solution hydrolysis, adjust the pH of the starch solution in the S1 tank so that the pH of the starch solution is 4.5, and at the same time increase the water bath heating of the tank, the heating temperature is 55 ° C, after the temperature of the solution in the tank is stable, Then add α-amylase to the tank, seal the tank through a parafilm, and continue the reaction for 4 hours to prepare solution A;

[0022] S3: Stevioside solution configuration, add an appropriate amount of stevia into solution A, and adjust the pH of solution A so that the pH value of solution A is 5.5, and at the sa...

Embodiment 2

[0031] A production method for obtaining 80% enzyme-modified stevioside of total glycosides through active enzyme deep reaction, comprising the following steps:

[0032] S1: Solution preparation, take a certain amount of pure water and starch into the tank, seal the opening of the tank through the sealing film, mix and stir to prepare a certain concentration of starch solution;

[0033] S2: solution hydrolysis, adjust the pH of the starch solution in the S1 tank so that the pH of the starch solution is 5, and at the same time increase the water bath heating of the tank, the heating temperature is 58 ° C, after the temperature of the solution in the tank is stable, Then add α-amylase to the tank, seal the tank through a parafilm, and continue the reaction for 5 hours to prepare solution A;

[0034] S3: Stevioside solution configuration, add a certain amount of stevioside to solution A, and adjust the pH of solution A so that the pH value of solution A is 6, and at the same time...

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PUM

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Abstract

The invention discloses a method for producing enzymatically modified stevioside with total glycoside content of 80% by using an active-enzyme deep reaction. The method comprises the following steps: preparation and hydrolysis of a solution; preparation of a stevioside solution; inactivation and filtering; adsorption and recovery; concentration and drying; and quality inspection and packaging. According to the invention, starch is hydrolyzed into cassava powder, and under the action of enzyme, glucosyl groups on the cassava powder are transferred to stevioside, so the sweet taste of stevioside is substantially improved; and hydrolysis temperature is strictly controlled by using a water bath, so high efficiency of hydrolase is guaranteed, the conversion rates of raw materials are improved, and the production cost of the enzymatically modified stevioside is lowered.

Description

technical field [0001] The invention relates to the technical field of stevioside, in particular to a production method for obtaining 80% enzymatically modified stevioside of total glycosides through deep reaction of active enzymes. Background technique [0002] Stevioside has no toxic side effects, no carcinogens, and is safe to eat. Regular consumption can prevent hypertension, diabetes, obesity and other diseases. It is an ideal sweetener to replace sucrose, but the existing production methods of stevioside have defects. For example, the conversion rate is low, and the extracted stevioside has a strong after-bitter taste, which limits the application of stevioside. Contents of the invention [0003] The purpose of the present invention is to solve the shortcomings in the prior art, and propose a production method for obtaining 80% enzyme-modified stevioside of total glycosides through deep reaction of active enzymes. [0004] In order to achieve the above object, the p...

Claims

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Application Information

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IPC IPC(8): C12P19/56C12P19/18C12P19/14
CPCC12P19/14C12P19/18C12P19/56
Inventor 张永逄坤忠夏正一夏修耀李存彪
Owner 安徽润海生物科技股份有限公司