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Application of kineococcus cavernae sp to tobacco quality improvement

A technology of kinetococcus and cave, which is applied in the fields of application, tobacco, bacteria, etc., and can solve the problems that there is no public report of kinetococcus.

Inactive Publication Date: 2017-09-12
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The Zoonococcus burrowing bacteria provided by the present invention was isolated for the first time, and was identified as a new species of microorganism. At present, there is no public report about the Zoonidiococcus burrowing bacteria and its use

Method used

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  • Application of kineococcus cavernae sp to tobacco quality improvement
  • Application of kineococcus cavernae sp to tobacco quality improvement
  • Application of kineococcus cavernae sp to tobacco quality improvement

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] ——Application of Kinetococcus elegans SYSUZL-1 in improving the quality of tobacco leaves, the specific steps are as follows:

[0023] (1) Slant culture: medium: glucose 0.5g; yeast extract 0.5g; peptone 0.5g; acid hydrolyzed casein 0.5g; soluble starch 0.5g; sodium pyruvate 0.3g; g; agar 15g; distilled water 1000ml, pH7.2; culture temperature is 28°C, culture time is 7d.

[0024] (2) Seed culture: pick part of the mycelium from the slope and put it into the seed liquid, and shake the flask for 36 hours. Seed medium: dextrin 120g; soybean powder 40g; yeast extract 2g; tryptophan 0.5g; β-alanine 5g; magnesium sulfate 0.5g; ammonium phosphate 0.2g; ℃.

[0025] (3) Fermentation culture: Inoculate 10% of the inoculum into the fermentation medium and culture in shake flasks for 6 days. Medium: soybean powder 10g; glucose 10g; peptone 3g; sodium chloride 2.5g; calcium carbonate 2g;

[0026] Take 1L of the culture solution, centrifuge at 3500r / min for 10min, remove the sup...

Embodiment 2

[0043] Repeat implementation 1, with the following differences: the treatment concentration is 20 mL per 1000 grams of shredded tobacco.

[0044] ——Chemical Composition Determination Results

[0045]The test results of the chemical components of the tobacco leaves are as follows (see Tables 4 and 5 for details). The experimental results show that, from the perspective of a single compound, the internal chemical components of the tobacco leaves treated with the bacterial agent have changed significantly. Compared with the control, the content of total sugar and reducing sugar increased greatly, and the content of total nitrogen, cellulose, protein and nicotine decreased compared with the control. Sugar-to-nitrogen ratio, sugar-to-base ratio, and nitrogen-to-base ratio all improved. The systematic test data shows that all kinds of shredded tobacco treated with bacterial agents are better than the control, and the components in the shredded tobacco tend to be balanced. It is be...

Embodiment 3

[0055] Repeat implementation 1, with the following differences: the treatment concentration is 30 mL per 1000 grams of shredded tobacco.

[0056] ——Chemical Composition Determination Results

[0057] The test results of the chemical components of the tobacco leaves are as follows (see Tables 7 and 8 for details). The experimental results show that, from the perspective of a single compound, the internal chemical components of the tobacco leaves treated with the bacterial agent have changed significantly. Compared with the control, the content of total sugar and reducing sugar increased greatly, and the content of total nitrogen, cellulose, protein and nicotine decreased compared with the control. Sugar-to-nitrogen ratio, sugar-to-base ratio, and nitrogen-to-base ratio all improved. The systematic test data shows that all kinds of shredded tobacco treated with bacterial agents are better than the control, and the components in the shredded tobacco tend to be balanced. It is b...

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Abstract

The invention discloses application of kineococcus cavernae sp to tobacco quality improvement. The strain named kineococcus cavernae sp is preserved in the China Center for Type Culture Collection on December 9, 2016, and a preservation number is M2016738. According to microbiological characteristic analysis and 16S rDNA gene sequence analysis, the strain is a novel bacterium. By spraying of an SYSUZL-1 bacterial agent to tobacco leaves prior to mellowing fermentation, tobacco aroma quality is improved, reducing sugar content is increased, contents of proteins, cellulose, total nitrogen and nicotine are decreased, and a sugar-nitrogen ratio, a sugar-nicotine ratio and a nitrogen-nicotine ratio are in increasing tendency, so that intrinsic chemical components of the tobacco leaves are harmonized, and organoleptic quality is improved evidently.

Description

technical field [0001] The invention belongs to the technical field of alcoholization and fermentation of tobacco, and in particular relates to the application of Kinetococcus tunica in improving the quality of tobacco leaves. Background technique [0002] Tobacco is one of the important pillars of our country's finances and has made great contributions to our country's social and economic development. In order to promote the progress of tobacco in our country and realize the sustainable development of the cigarette industry, we must take the road of tobacco technology innovation. The quality of tobacco leaves directly affects the intrinsic taste of cigarettes. The State Tobacco Monopoly Administration clearly pointed out that it is necessary to "overcome the key technologies that affect the aroma, quality and aroma of tobacco leaves" and narrow the quality gap with foreign tobacco leaves in order to improve domestic and foreign market competitiveness. [0003] Tobacco lea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B15/20C12R1/01
CPCA24B15/20C12N1/205C12R2001/01
Inventor 陈兴陈微段焰青夏建军李源栋张翼鹏党立志
Owner CHINA TOBACCO YUNNAN IND
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