A method and device for digital pcr
A digital and micro-pipe technology, applied in the field of digital PCR, can solve the problems of inconvenient carrying of digital PCR instruments, inconvenient carrying of digital PCR instruments, high cost of digital PCR instruments, etc., and achieves the effects of simple structure, easy operation and small size.
Active Publication Date: 2021-05-04
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology
[0003] At present, most digital PCR instruments require a separate droplet generating device (droplet generator) to generate droplets. The separate droplet generating device has a complex structure and a large size, which makes the digital PCR instrument inconvenient to carry. In the way of generating droplets, external equipment is generally used to provide power for the flow of oil phase and water phase. Due to the existence of external equipment, the problem of inconvenient portability of digital PCR instruments is also difficult to solve.
In addition, droplet generation equipment or external equipment to provide power will make the digital PCR machine expensive
Method used
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Embodiment 2
[0049] The difference between Embodiment 2 and Embodiment 3 and Embodiment 1 includes the difference in the PCR amplification area except that the four-way pipe 7 is changed to a Y-shaped pipe (tee pipe). In the second embodiment and the third embodiment, a part of the heat conduction sheet 9 is overlapped on the thermostat 8, and the remaining part is suspended outside the thermostat 8, and the micro-pipe 10 is arranged on the other side of the heat conduction sheet 9 opposite to the thermostat 8 , and the micro-pipe 10 reciprocates through the portion where the heat conduction sheet 9 overlaps the thermostat 8 and the portion that hangs outside the thermostat 8 in a preset number of cycles. Similar to Embodiment 1, the micro-pipe 10 may circulate 20 to 80 times on the surface of the heat conducting sheet 9 , for example, circulate 40 or 60 times.
[0050] Since Embodiment 2 and Embodiment 3 have adopted a Y-shaped pipe (tee pipe) to connect the oil phase container 2, the wat...
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The invention provides a method and device for digital PCR, the method is characterized in that, firstly, inflate and pressurize the system composed of the water phase container (6), the oil phase container (2) and the micropipe (10), Make the internal pressure of the system higher than the external pressure, then intercept a part of the high-pressure gas through the plugging of the water phase and the oil phase, and finally connect the end of the micropipe (10) to the outside of the system, so that the micropipe (10) A pressure difference is formed between the water phase container (6) and the oil phase container (2), so the water phase and the oil phase flow through the micropipe (10) under the action of the high-pressure gas. The method for digital PCR provided by the present invention avoids the use of external equipment to provide power for the flow of the oil phase and the water phase, so the digital PCR device designed according to the method is small in size, simple in structure, easy to carry, and has an economical price. , The advantages of easy operation.
Description
technical field [0001] The invention relates to the technical field of digital PCR, in particular to a method and device for digital PCR. Background technique [0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify and amplify specific DNA fragments. Droplet digital PCR needs to microdropletize the sample before amplification, and disperse the sample at one time. Into tens or even millions of micro-droplets to achieve the isolation of single-template molecules. [0003] At present, most digital PCR instruments require a separate droplet generating device (droplet generator) to generate droplets. The separate droplet generating device has a complex structure and a large size, which makes the digital PCR instrument inconvenient to carry. In the way of generating droplets, external equipment is generally used to provide power for the flow of oil phase and water phase. Due to the existence of external equipment, the problem of inconvenient port...
Claims
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IPC IPC(8): C12Q1/686C12M1/34C12M1/36
CPCC12Q1/686C12Q2563/159
Inventor 吴文明李渊明穆全全
Owner CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI