A kind of silkworm chrysalis protein, its complex protein and preparation method thereof

A silkworm chrysalis protein, silkworm chrysalis technology, applied in the direction of animal protein processing, protein food ingredients, protein food processing and other directions, can solve the problems of amino acid destruction and nutritional value reduction, and achieve the effects of reducing blood lipids, improving sleep, and solving yield

Active Publication Date: 2020-10-27
深圳市万欣生物智能科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The current silkworm chrysalis protein extraction method mainly has three characteristics. One is the hydrolysis process, such as using alkali hydrolysis, acid hydrolysis and enzymatic hydrolysis. It has a destructive effect on tryptophan and amino acids containing hydroxyl and sulfhydryl groups; in contrast, the enzymatic hydrolysis conditions are relatively mild, and various types of amino acids in proteins can be retained

Method used

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  • A kind of silkworm chrysalis protein, its complex protein and preparation method thereof
  • A kind of silkworm chrysalis protein, its complex protein and preparation method thereof
  • A kind of silkworm chrysalis protein, its complex protein and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 prepares silkworm chrysalis protein

[0043] S1. Silkworm chrysalis pretreatment: soak the silkworm chrysalis in hydrochloric acid, wash with water until the pH is neutral, and drain;

[0044] S2. Homogenization: the silkworm chrysalis obtained in S1 is mixed and stirred with water according to the solid-to-liquid ratio of 1:2, and the slag shell is filtered to obtain the silkworm chrysalis slurry;

[0045] S3. Enzymolysis: Enzyme is added to the silkworm chrysalis slurry obtained in S2 for enzymolysis. The enzyme is papain. The hydrolysis rate of the silkworm chrysalis slurry is 80-90%, the enzyme-to-substrate ratio is 2.5-4.5U / g, and the substrate concentration is 30-45g / L; the obtained enzymolyzed solution is placed in a boiling water bath to inactivate the enzyme;

[0046] S4. Degreasing: Centrifuge the enzymatic hydrolyzate obtained in S3 after deactivating the enzyme to separate the upper layer of fat to obtain a hydrolyzed protein solution;

[0047...

Embodiment 2

[0051] Embodiment 2 prepares silkworm chrysalis protein

[0052] S1. Silkworm chrysalis pretreatment: the silkworm chrysalis is soaked in acid, washed with water until the pH is neutral, and drained;

[0053] S2. Homogenization: the silkworm chrysalis obtained in S1 is mixed and stirred with water according to the solid-to-liquid ratio of 1:5, and the slag shell is filtered to obtain the silkworm chrysalis slurry;

[0054] S3. Enzymolysis: Enzyme is added to the silkworm chrysalis slurry obtained in S2 for enzymolysis, and the enzyme is neutral protease. The enzymatic hydrolysis method is as follows: use neutral protease to hydrolyze 3- After 4 hours, the hydrolysis rate of the silkworm chrysalis slurry is 40-60%, the enzyme-to-substrate ratio is 1000-4000U / g, and the substrate concentration is 45-55g / L; the obtained enzymolyzed solution is placed in a boiling water bath to inactivate the enzyme;

[0055] S4. Degreasing: Centrifuge the enzymatic hydrolyzate obtained in S3 aft...

Embodiment 3

[0060] Embodiment 3 prepares silkworm chrysalis protein

[0061] S1. Silkworm chrysalis pretreatment: the silkworm chrysalis is soaked in acid, washed with water until the pH is neutral, and drained;

[0062] S2. Homogenization: the silkworm chrysalis obtained in S1 is mixed and stirred with water according to the solid-to-liquid ratio of 1:3, and the slag shell is filtered to obtain the silkworm chrysalis slurry;

[0063] S3. Enzymolysis: Enzyme is added to the silkworm chrysalis slurry obtained in S2 for enzymolysis. The enzyme is trypsin. The hydrolysis rate of the silkworm chrysalis slurry is 60-80%, the enzyme-to-substrate ratio is 4000-7000U / g, and the substrate concentration is 45-55g / L; the obtained enzymatic solution is placed in a boiling water bath to inactivate the enzyme;

[0064] S4. Degreasing: Centrifuge the enzymatic hydrolyzate obtained in S3 after deactivating the enzyme to separate the upper layer of fat to obtain a hydrolyzed protein solution;

[0065] S...

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Abstract

The invention discloses silkworm pupa protein, compound protein thereof and a preparation method thereof. The preparation method of the silkworm pupa protein comprises the following technologies of silkworm pupa pretreatment, homogenate, enzymolysis, degreasing, decoloring, acid sedimentation, odor removing, drying and the like. The silkworm pupa protein can be compounded with soybean protein, whey protein or egg white protein; the compound protein can also be compounded with gamma-aminobutyric acid again. Compared with amino acid in protein with a single source, an amino acid proportion in the compounded protein is more close to an amino acid pattern needed by a human body, so that the compound protein can be more efficiently absorbed by the human body and has the functions of promoting growth and development of a body, increasing the body immunity, reducing serum cholesterol of a human and delaying senescence; further combination of the compounded protein and the gamma-aminobutyric acid has the functions of promoting sleep, strengthening the brain, benefitting intelligence, relieving restlessness, reducing blood pressure and the like.

Description

technical field [0001] The invention relates to the technical field of health food, in particular to a silkworm chrysalis protein, the invention also relates to a silkworm chrysalis protein compound, and the invention also relates to a preparation method of the compound. Background technique [0002] Silkworm chrysalis is the chrysalis of the Bombyx moth of the silk moth family insect, which is the by-product left after silk reeling. my country is the largest cocoon producing country in the world, and the silkworm chrysalis resources are very rich, with an annual output of more than 700,000 tons of silkworm cocoons. Generally, 1 ton of raw silk is produced, and about 0.8t of dry silkworm chrysalis is produced. However, the processing utilization rate of silkworm chrysalis in my country is extremely low, and most of them are used as feed or even Many fertilizers are discarded, and the utilization efficiency of silkworm chrysalis resources is very low. With the continuous deve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/17A23J1/02A23J3/34A23J3/04A23L33/175
CPCA23J1/02A23J3/341A23V2002/00A23L33/17A23L33/175A23V2200/324A23V2200/3262A23V2200/302A23V2250/542A23V2250/5488A23V2250/54252A23V2250/5428
Inventor 朱小松张小溪樊亚鸣
Owner 深圳市万欣生物智能科技有限公司
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