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Extracting and refining technology for heparin sodium in porcine intestinal mucosa

A technology of heparin sodium and mucous membranes, which is applied in the field of extraction and purification of heparin sodium in porcine intestinal mucous membranes, can solve the problems of not being able to meet a large number of needs, long production cycle, incomplete hydrolysis, etc., to improve and stabilize the yield and production cost of heparin sodium Low, well-designed effects

Inactive Publication Date: 2017-09-29
RUGAO YONGXING CASING
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  • Summary
  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0003] At present, in my country, pig small intestine is usually used to extract fresh intestinal mucosal fluid, and the traditional salt solution process is used to produce heparin sodium. Due to the long production cycle and incomplete hydrolysis of this preparation method, the obtained heparin sodium has low purity and low yield. Low, low potency, difficult to carry out large-scale production, and cannot meet the large demand of the market

Method used

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Embodiment Construction

[0020] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0021] The special enzymes for heparin sodium used in this example are the special enzymes for extracting heparin sodium produced by Nanning Dongheng Huadao Biotechnology Co., Ltd.

[0022] The extraction and refining technology of heparin sodium in pig intestinal mucosa, the steps are as follows:

[0023] (1) Pulp breaking: First, wash the fresh pig large intestine carefully with clean water to remove the internal and external dirt and external cortical fat, then mince it and grind it into a slurry, take 1000g of the pig large intestine slurry, add it under full stirring 1000g of water was mixed to obtain pig large intestine slurry;

[0024] (2) Enzymolysis: Under the condition of sufficient stirring, adjust the pH value of the slurry to 8...

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Abstract

The invention relates to an extracting and refining technology for heparin sodium in porcine intestinal mucosa. The extracting and refining technology comprises the following steps: smashing fresh pork intestines into slurry; preserving heat of the slurry for enzymatic hydrolysis; filtering enzymatic hydrolysate to collect filtrate; performing ion exchange adsorption treatment on the filtrate; washing and eluting with a resin; precipitating heparin sodium; drying to obtain crude heparin sodium. By adopting an enzymatic hydrolysis method, the pork intestine slurry can be dissolved more completely and fully, the product quality is effectively enhanced and stabilized, and the heparin sodium yield is increased; meanwhile, the prepared crude product is low in impurity content, and is high in titer.

Description

technical field [0001] The invention relates to a preparation technology for extracting heparin sodium from animal organs, in particular to a technology for extracting and refining heparin sodium in pig intestinal mucosa. Background technique [0002] Heparin sodium is a glucosaminoglycan widely present in animal organs (such as small intestinal mucosa, liver, pancreas, and lung tissue of cattle and sheep), and exists as a protein-bound complex in the body like most mucopolysaccharides , the anticoagulant activity of this complex increases with the removal of the protein. Heparin sodium and its derivatives are classic anticoagulant and antithrombotic drugs, which are widely used clinically to treat hemorrhage in patients with kidney disease, acute myocardial infarction, prevent thrombosis, cerebrovascular disease, skin disease, and eliminate the formation of kidney disease Heparin sodium also has a good effect in reducing blood fat and immunity, and with the gradual in-dept...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
CPCC08B37/0075
Inventor 费苹费正海
Owner RUGAO YONGXING CASING
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