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Preparation method of natural pigment of Xing'an azalea

A technology of natural pigment and Rhododendron, which is applied in the field of natural pigment preparation of Rhododendron xing'an, can solve the problem of less research on the extraction of Rhododendron xing'an anthocyanin, and achieve the effect of improving the extraction rate

Inactive Publication Date: 2017-09-29
BEIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, there have been reports on the extraction of anthocyanins by ultrasonic enhancement, but there are few studies on the extraction of anthocyanins from Rhododendron xing'an

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Fresh Rhododendron petals are cleaned and immersed in 90% ethanol at a liquid-to-material ratio of 8:1 to make them fully infiltrated; adjust the pH of the extract to 3; extract 20min under the ultrasonic power of 440W to obtain the extract; After vacuum filtration, the filtrate was centrifuged under the conditions of 6000rpm, 5min; the supernatant of the centrifugation was rotary evaporated at 40°C to remove the ethanol to obtain the crude extract, and kept in the dark. Use XAD-7 macroporous resin for separation and purification. When the pH is 3, the loading concentration is 0.035mg / ml. After equilibrating for 5 hours, it is loaded on the column at a flow rate of 2BV / h, and then eluted with 80% ethanol at a rate of 3BV / h to obtain a pure extract. The above pure extracts were pre-cooled at -80°C for 30 minutes, and vacuum freeze-dried at an absolute vacuum of 20MPa and -40°C for 24 hours to obtain natural pigment powder of Xing'an azalea.

Embodiment 2

[0024] Fresh Rhododendron petals are cleaned and immersed in 80% ethanol at a liquid-to-material ratio of 10:1 to make them fully infiltrated; adjust the pH of the extract to 3; extract with an ultrasonic power of 360W for 25 minutes to obtain the extract; After vacuum filtration, the filtrate was centrifuged under the conditions of 6000rpm, 5min; the supernatant of the centrifugation was rotary evaporated at 40°C to remove the ethanol to obtain the crude extract, and kept in the dark. Use XAD-7 macroporous resin for separation and purification. When the pH is 3, the loading concentration is 0.039mg / ml. After equilibrating for 5 hours, it is loaded on the column at a flow rate of 2BV / h, and then eluted with 80% ethanol at a rate of 3BV / h to obtain a pure extract. The above pure extracts were pre-cooled at -80°C for 30 minutes, and vacuum freeze-dried at an absolute vacuum of 20MPa and -40°C for 24 hours to obtain natural pigment powder of Xing'an azalea.

Embodiment 3

[0026] Fresh Rhododendron azalea petals are cleaned and immersed in 100% ethanol at a liquid-to-material ratio of 15:1 to make them fully infiltrated; adjust the pH of the extract to 3; extract for 30 minutes under the ultrasonic power of 280W to obtain the extract; After vacuum filtration, the filtrate was centrifuged under the conditions of 6000rpm, 5min; the supernatant of the centrifugation was rotary evaporated at 40°C to remove the ethanol to obtain the crude extract, and kept in the dark. Use XAD-7 macroporous resin for separation and purification. When the pH is 3, the loading concentration is 0.05mg / ml. After equilibrating for 5 hours, it is loaded on the column at a flow rate of 1 BV / h, and then eluted with 100% ethanol at a rate of 3 BV / h to obtain a pure extract. The above pure extracts were pre-cooled at -80°C for 30 minutes, and vacuum freeze-dried at an absolute vacuum of 20MPa and -40°C for 24 hours to obtain natural pigment powder of Xing'an azalea.

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Abstract

The invention provides a preparation method of a natural pigment of Xing'an azalea. The preparation method comprises the following steps: carrying out pretreatment on fresh Xing'an azalea petals, adding 95% ethanol according to a liquid-to-solid ratio of 10:1, soaking for 5 minutes at normal temperature, so that infiltration is sufficient; adjusting pH, and carrying out ultrasonic extraction for 20-30 minutes at a power of 320-360W; carrying out suction filtration, centrifugation and concentration, wherein anthocyanin content of the obtained crude extract is 56.1mg / 100g; and purifying with XAD-7 type macroporous resin under certain conditions, and carrying out vacuum freeze drying, so that a dry powder state is formed. The natural pigment prepared by adopting the preparation method is high in anthocyanin content and definite in pigment stability and can be widely applied to the field of food.

Description

Technical field [0001] The invention relates to a method for preparing natural pigments of Rhododendron xinanensis, belonging to the field of food additives and preparation methods thereof. Background technique [0002] Rhododendron dauricum L. . Rhododendron family, genus Rhododendron, is widely distributed in my country. Flowering in May, the first leaves of the flowers open, the corolla is broad and funnel-shaped, pink or purple, and the anthers and styles are purple. Studies have shown that azaleas not only have high ornamental and cultural value, but also have high economic value. [0003] In view of the safety issues of chemically synthesized pigments, the demand for natural pigments is becoming more and more extensive. As a type of natural water-soluble pigments widely distributed in plants, anthocyanin pigments are colorful, safe and rich in resources. They are excellent for developing natural food pigments. Plant-derived substances. Xing'an Rhododendron contains natural...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 张英莉巩超杜凤国
Owner BEIHUA UNIV