Unlock instant, AI-driven research and patent intelligence for your innovation.

Detection apparatus and detection method for optical density of microbes

A detection device and microbial technology, applied in enzymology/microbiology devices, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems of low accuracy, large interference, time-consuming, etc., and achieve accurate measurement results

Active Publication Date: 2017-10-03
承启医学(深圳)科技有限公司
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of this, the microbial optical density detection device and detection method disclosed in the present invention can effectively solve the technical defects of time-consuming, labor-intensive, low accuracy and large interference in traditional microbial growth curve measurement methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection apparatus and detection method for optical density of microbes
  • Detection apparatus and detection method for optical density of microbes
  • Detection apparatus and detection method for optical density of microbes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] see figure 1 , the present invention provides a microorganism optical density detection device, and embodiment 1 includes a horizontal bottom plate 1, a homogeneous light plate 2, a microbial culture plate 3, a vertical fixing part 4, and an RGB channel detection part 5; the vertical fixing part 4 is fixed and vertically arranged on the horizontal bottom plate One side of 1; the homogeneous light plate 2 is fixed on the upper surface of the horizontal bottom plate 1 of the fixed frame; the RGB channel detection unit is arranged on the top of the homogeneous light plate 2 through the vertical fixing part 4 of the fixed frame; the computing unit 6 and the RGB channel detection component 5 communication connection.

Embodiment 2

[0069] see figure 1 , the present invention provides a microorganism optical density detection device, and embodiment 2 includes a horizontal bottom plate 1, a homogeneous light plate 2, a microbial culture plate 3, a vertical fixing component 4, and an RGB channel detection component 5; the vertical fixing component 4 is fixed and vertically arranged on the horizontal bottom plate 1 side; the horizontal bottom plate 1 of the fixed frame contains a groove compatible with the homogeneous light plate 2; the homogeneous light plate 2 is fixed on the upper surface of the horizontal bottom plate 1 of the fixed frame; the RGB channel detection component 5 is fixed on the vertical fixed component 4 Above, the RGB channel detection part 5 needs to face the homogeneous light plate 2; the horizontal bottom plate 1 and the vertical fixing part 4 are both black acrylic frames, which can effectively prevent the light from being transmitted by the homogeneous light plate from scattering on t...

Embodiment 3

[0071] In order to evaluate the measurement accuracy of the OD value of the microbial culture solution of the detection device and method of the present invention, a spectrophotometer is used to measure the OD value of the microorganism, and then the OD value of the same microorganism is measured using the detection device and the detection method of the present invention. Correlation analysis is performed on the two measurement results, so as to evaluate the accuracy of the measurement results of the present invention and the correspondence with the measurement values ​​of traditional precision instruments.

[0072] 1. Cultivate E. coli BW25113 overnight, and dilute E. coli BW25113 in 13 different concentrations with medium.

[0073] 2. Use a Thermo spectrophotometer to measure the OD of 13 different concentrations of E. coli BW25113 in step 1 600 value.

[0074] 3. use the Auto24OD value of the escherichia coli E.coli BW25113 of 13 different concentrations in the detection ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a detection apparatus and detection method for the optical density of microbes, belonging to the field of detection of the growth of microbes. The detection apparatus comprises a fixed mount, a homogeneous light plate, a microbe culture plate and a RGB channel detection part, wherein the fixed mount comprises a transverse base plate and at least one vertical fixed part; the homogeneous light plate is arranged on the upper surface of the transverse base plate of the fixed mount; the microbe culture plate is fixedly arranged above the homogeneous light plate; and the RGB channel detection part is arranged above the homogeneous light plate via the vertical fixed part of the fixed mount and used for measuring the intensity I of transmitted light in each pore of the microbe culture plate. The detection apparatus further comprises an arithmetic unit which calculates the Auto OD value of each pore in the microbe culture plate based on a correction coefficient F corresponding to the pore and a preset formula. The detection apparatus and detection method provided by the invention can effectively overcome the technical defects of great consumption of time and manpower, low accuracy and great interference in traditional measurement methods for the growth of microbes.

Description

technical field [0001] The invention belongs to the field of microbe optical density monitoring, and in particular relates to a microbe optical density detection device and a detection method. Background technique [0002] Research on microbial fermentation and bacterial drug resistance are two important directions in the fields of industrial fermentation and biomedicine respectively. However, both microbial fermentation and bacterial drug resistance research require the detection of the activity of the strains. Detection is the determination of the growth rate of the strain. [0003] The determination of the microbial growth curve is an important indicator to reflect the activity of microbial strains in the field of microbial-related functional research. The growth curve is an experimental curve that quantitatively describes the growth law of the microbial population in the liquid medium. When a small amount of pure-bred single-cell microorganisms are inoculated into cons...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12M1/34C12Q1/04
CPCC12M41/36C12Q1/04
Inventor 杜高飞钟嘉泳
Owner 承启医学(深圳)科技有限公司