Sealed poultry and livestock house internal environment microbe aerosol monitoring method

A technology for livestock and poultry houses and aerosols, which is applied in the measurement/inspection of microorganisms, biochemical equipment and methods, and special data processing applications, etc. Sampling requirements, low sampling efficiency and other issues, to achieve good protection effect

Active Publication Date: 2017-10-03
SOUTHWEAT UNIV OF SCI & TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study of microbial aerosols needs to be sampled first, but the current sampling efficiency of microbial aerosols is low, the sampling time is long, and the sampling quality is poor, which cannot meet the requirements of rapid, efficient and high-quality sampling of microbial aerosols.

Method used

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  • Sealed poultry and livestock house internal environment microbe aerosol monitoring method
  • Sealed poultry and livestock house internal environment microbe aerosol monitoring method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for monitoring environmental microbial aerosols in a closed livestock and poultry house, comprising the following steps:

[0027] Step 1. Arrangement of sampling points: In the net bed pig house, the points are arranged according to the method of selecting points on the four equal diagonal lines, and at least 5 points are set at the sampling points; From the center of the house as the starting point, select equal sampling points from the starting point to the four corners of the net bed pig house, and use the equal sampling points and the central point of the livestock and poultry house to form 5 sampling points; figure 1 As shown, three-layer sampling is selected for sampling, and the heights are respectively 0.2m for the first layer 1, 0.4m for the second layer 2 and 0.6m for the third layer 3;

[0028] Step 2. Continuously sample the sampling points through the air microbial aerosol sampling device in the net-bed pig house, use metagenomic technology to anal...

Embodiment 2

[0030] A method for monitoring environmental microbial aerosols in a closed livestock and poultry house, comprising the following steps:

[0031]Step 1. Arrangement of sampling points: In the caged laying hen house, the points are arranged according to the method of quartering the diagonal line selection, and the sampling points are set at least 5 points; The center of the laying hen house is used as the starting point, and equal sampling points are selected from the starting point to the direction of the four corners of the caged laying hen house, and five sampling points are composed of equal sampling points and the center point of the caged laying hen house; three layers are selected for sampling Sampling, the heights are 0.8m, 1m and 1.2m respectively;

[0032] Step 2. Continuously sample the sampling points through the air microbial aerosol sampling device in the caged layer house, use the metagenomic technology to analyze the type and content of microorganisms, and combi...

Embodiment 3

[0034] A method for monitoring environmental microbial aerosols in a closed livestock and poultry house, comprising the following steps:

[0035] Step 1. Arrangement of sampling points: in the ground breeding duck house, the points are arranged according to the method of quadrangling the diagonal line selection, and at least 5 sampling points are set; Take the center of the house as the starting point, select equal sampling points from the starting point to the four corners of the ground breeding duck house, and use the equal sampling point and the center point of the ground breeding duck house to form 5 sampling points; select three-layer sampling for sampling, and the heights are respectively 0.1m, 0.3m and 0.4m;

[0036] Step 2. Continuously sample the sampling points through the air microbial aerosol sampling device in the duck house on the ground, use the metagenomic technology to analyze the type and content of microorganisms, and combine bioinformatics to obtain dominan...

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Abstract

The invention discloses a sealed poultry and livestock house internal environment microbe aerosol monitoring method, which comprises the following steps that (1) sample points are arranged: point distribution is performed in a poultry and livestock house by a quartering diagonal line point selection method; at least five sampling points are set; (2) the continuous sampling is performed on the sampling points in the poultry and livestock house by an air microbe aerosol sampling device; a metagenome technology is used for analyzing the microbe kinds and contents; the bioinformatics is combined for obtaining information of dominant bacteria, pathogenic bacteria and potential pathogenic bacteria, wherein the continuous sampling process comprises the sampling during the behaviors of feed feeding, feed taking peak period, poultry and livestock resting, poultry and livestock free activity, poultry and livestock house cleaning and pad material replacement.

Description

technical field [0001] The invention relates to a method for monitoring microbial aerosols, in particular to a method for monitoring environmental microbial aerosols in closed livestock and poultry houses. Background technique [0002] my country is a large livestock and poultry breeding country, with a large number and variety of livestock and poultry. Their growth is affected by genetics, products, feed, diseases and the environment. A large number of scientific production and practical experience have confirmed that environmental impact factors account for 30%, and with the rapid development of intensive and large-scale farming, the proportion of environmental impact will become larger and larger. Especially in recent years, due to the large number of closed livestock and poultry houses, the environment in the house has become more and more complicated. The frequent outbreak and spread of livestock and poultry epidemic diseases such as foot-and-mouth disease, swine unknow...

Claims

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Application Information

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IPC IPC(8): C12Q1/24G06F19/00G06Q50/02
CPCC12Q1/24G06Q50/02G16Z99/00
Inventor 刘静波余马刘林娜郭振东欧洋谢强
Owner SOUTHWEAT UNIV OF SCI & TECH
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