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Spectral unmixing detection method for in-vivo fluorescence

A technology of in vivo fluorescence and spectral unmixing, which can be used in fluorescence/phosphorescence, measurement devices, material analysis by optical means, etc., and can solve problems such as the lack of industry consensus.

Active Publication Date: 2017-10-10
DALIAN MARITIME UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In this field, it is still in the exploratory stage, and there is no effective and reasonable calculation method that has formed an industry consensus

Method used

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  • Spectral unmixing detection method for in-vivo fluorescence
  • Spectral unmixing detection method for in-vivo fluorescence
  • Spectral unmixing detection method for in-vivo fluorescence

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Embodiment 1

[0087] This experiment uses two sets of image data, the size of which is 1040×1392×11 and 520×696×27, which are the color band test image and the white mouse fluorescence image respectively. Ribbon test image such as image 3 As shown, the fluorescence image of white mouse is shown as Figure 4 shown.

[0088] The present invention researches and designs a method for detecting and analyzing fluorescence distribution using hyperspectral unmixing technology, which eliminates the influence of living autofluorescence in two different ways, and uses weighting and automatic determination of weight coefficients, with the help of two detection methods And the unmixing method LSE, LSOSP, carried out the experiment respectively with strip test data and live white mouse fluorescence data, verified the effectiveness of the method proposed by the present invention.

[0089] According to the method of spectral angle matching, the detection situation of each color band can be obtained as f...

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Abstract

The invention discloses a spectral unmixing detection method for in-vivo fluorescence. The spectral unmixing detection method comprises the following steps: acquiring a to-be-analyzed in-vivo fluorescence image, analyzing a mixed fluorescence spectrum of the in-vivo fluorescence, and establishing a linear spectrum mixing model of a current fluorescence image according to a spontaneous fluorescence spectrum and a target fluorescence spectrum; and establishing an orthogonal subspace projection of the linear spectrum mixing model, and combining with a least squares algorithm to eliminate the spontaneous fluorescence spectrum in the linear spectrum mixing model, so as to obtain an interested spectrum, namely the target fluorescence spectrum in a nonseparable manner.

Description

technical field [0001] The present invention relates to a spectral unmixing detection method of living body fluorescence, G06 calculation; calculation; counting G06T general image data processing or generation G06T7 / 00 image analysis, for example, from position image to non-position image. Background technique [0002] In vivo fluorescence technology can simulate the localization effect of targeted drugs on specific tumors in biomedicine, so as to analyze the performance of drugs and help in real-time surgical guidance. The realization of this function relies on the accurate resolution and detection of living fluorescence. [0003] In this field, it is still in the exploratory stage, and there is no method that is both effective and has a reasonable amount of calculation that has reached an industry consensus. [0004] Hyperspectral remote sensing is now widely used in various fields, because high spatial resolution and spectral resolution make it provide more information f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6486G01N2021/6423
Inventor 宋梅萍薛白于纯妍张建祎李森王玉磊
Owner DALIAN MARITIME UNIVERSITY