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Microfluidic system and method for perfusion bioreactor cell retention

A microfluidic system, bioreactor technology, applied in specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, biochemical equipment and methods, etc.

Inactive Publication Date: 2017-10-13
MASSACHUSETTS INST OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the optimal frequency and flow rate for each cell type must be adjusted, and there is no industrial scale use of this technique in perfusion culture

Method used

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  • Microfluidic system and method for perfusion bioreactor cell retention
  • Microfluidic system and method for perfusion bioreactor cell retention
  • Microfluidic system and method for perfusion bioreactor cell retention

Examples

Experimental program
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Embodiment Construction

[0035] Exemplary embodiments are described below.

[0036] One embodiment of the present invention provides a membrane-free and non-clogging microfiltration platform that uses inertial microfluidics to perform ultra-high throughput (at the level of liters / minute) of cell separation with extremely high throughput. The system developed according to one embodiment of the present invention is a highly multiplexed microfluidic device, which consists of a multilayer PDMS sheet with concave and convex microchannels (ie, up to 500 spirals), which are combined together for use from large Volumetric biological samples continuously separate cells based on size. This technology uses the hydrodynamic forces existing in the curved microchannels to concentrate and sort cells.

[0037] In a system according to an embodiment of the present invention, the cells are separated only due to the fluid interaction driven by the external driving fluid, so the system is essentially non-blocking and does no...

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Abstract

A microfluidic system for cell retention for a perfusion bioreactor is provided. The system comprises at least one inlet configured to receive a bioreaction mixture to be processed. At least one curvilinear microchannel is in fluid flow connection with the at least one inlet, the at least one curvilinear microchannel being adapted to isolate cells in the bioreaction mixture, based on cell size, along at least one portion of a cross-section of the at least one curvilinear microchannel. At least two outlets are in fluid flow connection with the at least one curvilinear microchannel. At least one outlet of the at least two outlets is configured to flow the isolated cells to be recycled to the perfusion bioreactor.

Description

[0001] Related application [0002] This application claims the benefits of U.S. Provisional Application No. 62 / 051,497 filed on September 17, 2014, the entire contents of which are incorporated herein by reference. [0003] governmental support [0004] This invention was completed with government support under DE-AR0000294 granted by ARPA-E, and is entitled "Scalable, Self-Powered Purification Technology for Brackish and Heavy-Purification Technology for Brackish and Heavy Metal Polluted Water. Metal Contaminated Water)". The U.S. government has certain rights in this invention. Background technique [0005] Mammalian cell cultures are widely used to manufacture large and complex chemicals, such as drugs and proteins used in biotechnology and medicine [1]. The increasing demand for these products has led to a continuous push for "upstream" (bioreactor operation) improvements [2]. For this purpose, perfusion bioreactors have been widely used because they can maintain high cell num...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M3/00C12M3/04C12N5/02
CPCC12M23/02C12M23/16C12M47/04C12M47/02C12M29/10C12M29/18B01L3/502753B01L3/502761B01L2200/0647B01L2300/0681B01L2300/0848B01L2300/0861C12M29/04
Inventor M·埃博拉西米沃齐亚尼韩宗润K·P·A·泰关国峰
Owner MASSACHUSETTS INST OF TECH