Device and method for digital PCR

Abstract

The invention provides a device and method for digital PCR. The device comprises a water phase container (1), an oil phase container (2), a micro pipeline (4), and a collection bottle (7). The volumes of the water phase container (1) and the oil phase container (2) are adjustable. One end of the micro pipeline (4) is communicated with the water phase container (1) and the oil phase container (2). The other end of the micro pipeline (4) is provided with a first vent valve (5). The collection bottle (7) is communicated with the first vent valve (5). An exhaust valve (6) is arranged on the collection bottle (7). The device can automatically generate liquid drops and drive liquid drops to flow without any external power supply. According to the relationship between the flowing speed of liquid in the micro pipeline (4) and the liquid viscosity, pipeline diameter, pipeline length, and the pressure difference between the tail end and head end of the pipeline, the flowing speed of the liquid can be controlled, the pressure in the collection bottle (7) can be changed, and the whole system is operated under situation that the pressure of the tail end is equal to and higher than the atmospheric pressure.

Description

technical field [0001] The invention relates to the technical field of digital PCR quantification, in particular to a device and method for digital PCR. Background technique [0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. The generation of micro-droplets is an important part of micro-droplet digital PCR technology. Currently, commonly used micro-droplets There are two main production methods: 1. Put the oil phase and the water phase into the same container, and use physical or chemical methods to make the water phase form droplets in the oil phase; 2. Put the oil phase and the water phase separately In the two containers, the water phase and the oil phase are driven to flow, the oil phase and the water phase are mixed in the pipeline, and the shear force of the two phases is used to form droplets. [0003] In order to implement the above two methods, relatively complex equipment and external energy drive are ...

AI Technical Summary

Problems solved by technology

[0003] In order to implement the above two methods, relatively complex equipment and external energy drive are usually required, while the control of droplets is mostly realized through control valves and complex control systems. The equipment is expensive and bulky, and is not suitable for large-scale promotion.

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