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Screening method of benzene pollutant BTEX degrading bacteria

A technology of benzene-based pollutants and a screening method is applied in the field of screening of benzene-based pollutants BTEX degrading bacteria, which can solve the problems of large workload, long time, and low screening efficiency, and achieves small workload, strong pertinence, and screening. Efficient effect

Inactive Publication Date: 2017-10-27
SUZHOU ZHONGSHENG ENVIRONMENTAL REMEDIATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional bacteria screening methods mostly use pollutants as the only carbon source for cultivation and screening. Although they are highly targeted, they have a large workload, long time, and low screening efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The method for screening BTEX degrading bacteria of benzene series pollutants provided by the present invention includes two stages of preliminary screening and re-screening, and after optimizing culture factors, strains with high-efficiency degradation characteristics are obtained;

[0066] The specific operation includes the following steps:

[0067] S1. Collection of bacteria source: taken from a long-term idle land after the relocation of an agrochemical plant. The preliminary investigation found that there are serious benzene series (benzene, toluene, xylene) pollution in the soil in some areas of the site. With a production history of more than 30 years, the factory has been left unoccupied for nearly 10 years after relocation. The 0.2m depth soil in this area is selected as the source of benzene-based pollutant degrading bacteria. Multi-point sampling is carried out to collect a number of soil for screening, and 1.5 soil samples are taken from a single point. about kg...

Embodiment 2

[0075] The method for screening BTEX degrading bacteria of benzene series pollutants provided by the present invention includes two stages of preliminary screening and re-screening, and after optimizing culture factors, strains with high-efficiency degradation characteristics are obtained;

[0076] The specific operation includes the following steps:

[0077] S1. Collection of bacteria source: taken from a long-term idle land after the relocation of an agrochemical plant. The preliminary investigation found that there are serious benzene series (benzene, toluene, xylene) pollution in the soil in some areas of the site. With a production history of more than 30 years, the plant has been left unoccupied for nearly 10 years after the relocation. The soil in this area is selected to be 0.3m deep as the source of benzene-based pollutant degrading bacteria to screen the bacteria. Multi-point sampling is carried out to collect several soils for screening, and 1.5 soil samples are taken fro...

Embodiment 3

[0085] The method for screening BTEX degrading bacteria of benzene series pollutants provided by the present invention includes two stages of preliminary screening and re-screening, and after optimizing culture factors, strains with high-efficiency degradation characteristics are obtained;

[0086] The specific operation includes the following steps:

[0087] S1. Collection of bacteria source: taken from a long-term idle land after the relocation of an agrochemical plant. The preliminary investigation found that there are serious benzene series (benzene, toluene, xylene) pollution in the soil in some areas of the site. With a production history of more than 30 years, the plant has been left unoccupied for nearly 10 years after relocation. The soil in this area is selected as the source of benzene-based pollutant degrading bacteria to screen the bacteria. Multi-point sampling is carried out to collect a number of soil for screening, and a soil sample of 1.5 is taken from a single poi...

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Abstract

The invention discloses a screening method of benzene pollutant BTEX degrading bacteria. The screening method comprises the following steps: collecting a bacterial source; adding bacterial source soil into a conical bottle with sterile water, shaking, and standing; smearing supernatant onto a primary screened culture medium flat plate to be cultured; selecting cultured rusty red oxidized sphere bacterial colony, and concentrating and culturing the bacterial colony in a liquid culture medium; smearing a culture solution on a secondary screened culture medium flat plate to be cultured; scribing and purifying the cultured transparent halo bacterial colony; and inoculating the purified bacterial colony onto an inclined surface of a strain preservation culture medium, and storing in a refrigerator for standby use. The screening method is high in screening efficiency and small in workload; the obtained degrading bacteria have high pertinence, have good degrading and removal effect for benzene pollutants in the soil, are used for restoring the soil polluted by the benzene pollutant BTEX, have the advantages of short restoration time, high restoration efficiency, low restoration cost, environmental friendliness, no pollution and the like, and is worth of popularization.

Description

Technical field [0001] The invention relates to the technical field of microbial remediation of organic contaminated soil, in particular to a method for screening BTEX degrading bacteria of benzene series pollutants. Background technique [0002] Benzene-based pollutants (BTEX) are a type of organic pollutants with greater toxicity and carcinogenicity. They are often used in large quantities as raw and auxiliary materials or organic solvents in the chemical industry. Due to its strong hydrophobicity, it is easy to be adsorbed and enriched in the soil. At the same time, due to its small specific gravity and strong volatility, it is more harmful to human health and the ecological environment. [0003] Regarding the contaminated soil caused by benzene series pollutants (BTEX), in the prior art, conventional remediation methods include gas phase extraction, thermal desorption, cement kiln co-processing, chemical oxidation and other logistics chemical measures, but the above treatment m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02B09C1/10
CPCB09C1/10C12N1/02
Inventor 吴廷辉盛月慧潘澄
Owner SUZHOU ZHONGSHENG ENVIRONMENTAL REMEDIATION CO LTD
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