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Pig chromosome 12 snp markers and detection methods related to the litter size of Erhualian pigs

A technology of Erhualian pigs and Erhualian sows, which is applied in the field of molecular biology, can solve the problems of less effective utilization and achieve important economic benefits and social value

Active Publication Date: 2021-02-02
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Although a large number of domestic research institutions have been trying to identify the genetic mechanism of the high fecundity of Erhualian pig breeds for many years, with the changes in research methods, means, materials and the population itself, there are new Erhualian pig breeds with high fecundity. Genetic mechanisms revealed, but less effectively exploited

Method used

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  • Pig chromosome 12 snp markers and detection methods related to the litter size of Erhualian pigs
  • Pig chromosome 12 snp markers and detection methods related to the litter size of Erhualian pigs
  • Pig chromosome 12 snp markers and detection methods related to the litter size of Erhualian pigs

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Experimental program
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Effect test

Embodiment 1

[0028] 1. Source of experimental animals

[0029] Erhualian sows come from 143 purebred Erhualian sows from three bases of Changzhou Jiaoxi Erhualian Pig Professional Cooperative; 71 purebred Erhualian sows from Suzhou Sutai Enterprise; 90 purebred Erhualian pig breeding farms in Changshu City, Jiangsu Province The first purebred Erhualian sow, a total of 304 heads.

[0030] 2. Genomic DNA extraction

[0031] Ear tissue samples from 304 sows were collected, placed in centrifuge tubes filled with 70% alcohol, and stored in a -20°C refrigerator for later use.

[0032] Using the traditional phenol / chloroform method to extract genomic DNA from ear tissue, the required reagents include:

[0033] Lysate laboratory equipment

[0034] Proteinase K (Germany MERCK Biotechnology Co., Ltd.)

[0035] Tris Saturated Phenol (Beijing Suolaibao Biotechnology Co., Ltd.)

[0036] Tris Saturated Phenol: Chloroform: Isoamyl Alcohol (25:24:1) (Beijing Suo Laibao Biotechnology Co., Ltd.)

[00...

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Abstract

The invention relates to a SNP marker of pig chromosome 12 and a detection method related to the litter size of Erhualian pigs. The SNP marker is located on the nucleotide sequence of the paired ribosomal protein kinase RPS6KB1 gene on pig chromosome 12, and its site is the g.37433196 nucleotide site on pig chromosome 12 of the International Porcine Genome Version 10.2 Reference Sequence , and has a G / C polymorphism, the SNP marker is significantly correlated with the total litter size of Erhualian sows, and is extremely significantly correlated with the number of live children born in the litter. A pair of primers for detecting the SNP marker, the upstream primer is: SEQ ID NO: 2, and the downstream primer is: SEQ ID NO: 3. The SNP marker provided by the present invention is related to the farrowing performance of Erhualian sows, and the high-yielding Erhualian sow strain can be produced by identifying the SNP marker, and the obtained high-productivity Erhualian sow line has important economic benefits and social value .

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a SNP marker of pig chromosome 12 and a detection method related to the litter size of Erhualian pigs. Background technique [0002] Litter size is one of the most important production traits of pigs. It is an important breeding index in the selection of commercial strains of pigs, and is directly related to the economic benefits of the pig industry. As a complex quantitative trait, litter size has low heritability and is influenced by many factors. From before ovulation to after farrowing, various factors such as the breed, age, parity of the sow, the quality of semen with the boar and the nutritional status of the sow during pregnancy will affect the litter size of the sow to varying degrees. influences. However, the progress of traditional breeding methods to increase piglet size is slow, which can no longer meet the production needs. The advent and application of M...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 黄瑞华李平华方宇瑜马翔张倩
Owner NANJING AGRICULTURAL UNIVERSITY