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Method for performing supersensitive detection on mercury ions by using T-Hg(II)-T structure-based AgNCs probe

A sensitive detection and mercury ion technology, applied in the field of biosensing, can solve the problems of low detection accuracy of mercury ions, narrow detection linearity, complex synthesis, etc., and achieve the effect of wide detection concentration range, low detection limit and high sensitivity

Active Publication Date: 2017-10-27
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest defect of this type of method is that the detection accuracy of mercury ions by fluorescence quenching is low. In addition, fluorescent probes labeled with fluorescein are required, which are costly, complex to synthesize, high detection limits, and narrow detection linearity.

Method used

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  • Method for performing supersensitive detection on mercury ions by using T-Hg(II)-T structure-based AgNCs probe
  • Method for performing supersensitive detection on mercury ions by using T-Hg(II)-T structure-based AgNCs probe
  • Method for performing supersensitive detection on mercury ions by using T-Hg(II)-T structure-based AgNCs probe

Examples

Experimental program
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Effect test

Embodiment 1

[0035] The DNA1 sequence number adopted is: 5'-TTTTTTCCCCCCTAATTCCCAAAGCTCGACGGATT-3';

[0036] The DNA2 sequence number used is: 5'-AATCCGTCGAGCAGGGGAAGGGGAAGGGTTTTTT-3';

[0037] The preparation method of DNA1-AgNCs, the steps are as follows:

[0038] Take 33 μL of DNA1 (100 μM), and dissolve DNA1 with pH=7.4, 10 mM phosphate buffer (the DNA1 solution that has been centrifuged before use is stored in a refrigerator at 4°C). Next, 160 μL phosphate buffer (20 mM, pH=7.0) was mixed with the above 33 μL DNA1, and 13.2 μL AgNO was added to the mixed solution. 3 Aqueous solution (1.5mM), the whole mixed solution above was continuously shaken with a mixer at room temperature for 15min (1000r / min), and then 13.2μL of freshly prepared NaBH was taken 4 (1.5mM) solution was quickly added to the above mixed solution, and the whole solution was mixed for 30s, and then the whole solution was placed in a constant temperature water bath at 25°C for 2h. After the reaction was completed, th...

Embodiment 2

[0052] The operation steps and experimental conditions are the same as in Example 1, except that the sequence number of DNA2 is replaced by 5'-AATCCGTCGAGCAGGGGAAGGGGAAGGGTTTTTTCGACGACGACGACGACGACGACGACGACGA-3'.

[0053] from Figure 5 It can be seen that Hg in the concentration range of 0.00002nM ~ 1.6nM 2+ It has a certain linear relationship with its corresponding response signal.

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Abstract

The invention a method for performing supersensitive detection on mercury ions by using a T-Hg(II)-T structure-based AgNCs probe. The method comprises the following steps: synthesizing a siliver nano-cluster (DNA1-AgNCs) by taking DNA1 as a template, performing hybrid combination on the DNA1-AgNCs and DNA2 to obtain a DNA1 / DNA2-AgNCs fluorescent probe, and detecting the Hg(II) concentration of the solution by utilizing the fluorescence change of the DNA1 / DNA2-AgNCs fluorescent probe. The method has the advantages of label-free property, strong signal, high specificity, high sensitivity, wide detection concentration range, low detection limit and the like.

Description

technical field [0001] The present invention relates to a kind of Hg 2+ A detection method, in particular to a DNA1 / DNA2-AgNCs fluorescent probe based on DNA as a template, and using the DNA1 / DNA2-AgNCs fluorescent probe to detect Hg 2+ A method for specific fluorescence detection belongs to the technical field of biosensing. Background technique [0002] Heavy metal mercury ions exist widely in nature in the form of inorganic salts, organic complexes and metallic mercury. Mercury ions easily accumulate in the body through the food chain, resulting in permanent damage to the brain, liver and central nervous system. The US Environmental Protection Agency (EPA) strictly defines the concentration of mercury ions in drinking water as ≤10nM. At present, the trace detection of metal mercury ions has become an urgent problem. The traditional and reliable detection methods of metal mercury ions are mainly based on atomic absorption / emission spectrometry. However, such methods ar...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/65
CPCC09K11/65G01N21/6428
Inventor 邓春艳丁亚林
Owner CENT SOUTH UNIV
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