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Monodisperse nanolamella-based nanometer single hole, and making method and application thereof

A nanosheet, monodisperse technology, applied in the field of nanopores, to achieve the effects of stable device structure, mild conditions and great application value

Active Publication Date: 2017-11-17
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, surface-enhanced Raman also has inherent limitations. For complex samples, all molecules in the laser spot will be enhanced by the Raman substrate. To obtain single-molecule information from complex samples, the Raman signals of other molecules must be filtered out.

Method used

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  • Monodisperse nanolamella-based nanometer single hole, and making method and application thereof
  • Monodisperse nanolamella-based nanometer single hole, and making method and application thereof
  • Monodisperse nanolamella-based nanometer single hole, and making method and application thereof

Examples

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Effect test

Embodiment 1

[0040] The method for preparing nano single hole of the present invention, comprises the steps:

[0041] (1) Preparation of the tip of the glass capillary: the instrument used is a P-2000 needle puller from SUTTER, USA, and the glass capillary is BOROSILIGATE GLASS WITH FILAMENT from SUTTER, O.D: 1.0mm, I.D: 0.58mm, and the total length is 10cm. Set parameters as Heat 350,fil 4,vel 33,del 250,pull 200

[0042] The diameter of the tip of the capillary prepared under the above conditions with the capillary of this specification is 50-100nm, such as image 3 shown.

[0043] (2) Preparation of Silver Triangle Tablets: Fix the total volume of the solution at 25ml, add 50ul silver nitrate (0.05M), 0.5ml trisodium citrate dihydrate (75mM), 100ul PVPK-30 (Mw≈ 40,000g / mol, 17.5mM), 60ul H 2 o 2 (30wt%), stirred vigorously, then quickly added 150ul NaBH 4 (100mM), the solution quickly changed from colorless to light yellow, and after about 25min, the light yellow turned to dark yel...

Embodiment 2

[0051] The difference between Example 2 and Example 1 is that in this example, gold nanotriangles are used to prepare a single-pore structure, and the single-layer gold nanoparticles are fixed on the tip of a glass capillary using DNA with thiol groups at both ends as a linker. Such as Figure 9 shown. The prepared single hole has a pore diameter of 60 nm.

Embodiment 3

[0053] The difference between Example 3 and Example 1 is that in this example, a copper nanohexagonal sheet is used to prepare a single-hole structure, and polydopamine is used as a linker to fix copper nanoparticles on the tip of a glass capillary, and the self-assembly time is 2 hours. The prepared single hole has a pore diameter of 100 nm.

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Abstract

The invention relates to a monodisperse nanolamella-based nanometer single hole, and a making method and an application thereof. The nanometer single hole is positioned in the nanometer tip of a capillary tube, and is formed by a monodisperse nanolamella capping the tip of the capillary tube, and the aperture of the nanometer single hole is 10-100 nm. The metal nanometer single hole has the advantages of simple making method, low cost, small dimension and controllable structure, and can be combined with Raman spectrum as an electrochemical device to realize electrochemical controllable single molecule detection and realize the identification of single bases, so a method is provided for DNA sequencing and even protein sequencing.

Description

technical field [0001] The invention belongs to the technical field of nanopores, and specifically designs a nano single-pore structure formed by using monodisperse nano-sheets, a preparation method and application thereof. Background technique [0002] With the gradual deepening of human understanding of life activities, single-molecule detection has received more and more attention. Nanopore analysis technology is currently the youngest single-molecule detection technology. When the pore size is close to the size of the analyte, the molecules of the analyte passing through the nanopore will generate a large ion current change, thereby realizing the detection of a single molecule or ion. The detection conditions of the nanopore analysis technology are mild, no pretreatment is required for the detection object, and the detection process does not destroy the physical and chemical properties of the detection object. Although nanopore analysis technology has made great achieve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65B82Y15/00B82Y30/00B82Y40/00
CPCG01N21/658B82Y15/00B82Y30/00B82Y40/00
Inventor 王康曹姣刘海玲夏兴华
Owner NANJING UNIV
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