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Preparation method of reagent for detection of monohydroxyphenol derivatives by sulfonation reaction

A technology for detecting reagents and monohydric phenols, which is applied in the field of reagents and preparations for detecting monohydric phenol derivatives in urine, and can solve problems such as accuracy of processing problems

Active Publication Date: 2017-11-17
青岛浩铂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the shortcomings of the above method, the present invention provides a reagent preparation method for detecting monohydric phenol derivatives by sulfonation reaction, in order to solve the problems of reagent configuration and post-use treatment and low accuracy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. take by weighing mercuric sulfate and be dissolved in the sulfuric acid aqueous solution of 10% mass concentration and obtain A solution, in the solution, mercuric sulfate content is 400g / l;

[0020] 2. Measure a certain volume of chlorosulfonic acid and dissolve it in deionized water to obtain solution B. The content of chlorosulfonic acid in the solution is 100ml / l;

[0021] 3. Take a certain volume of sulfolane and dissolve it in deionized water to obtain solution C. The content of sulfolane in the solution is 100ml / l;

[0022] 4. Mixing: Mix the prepared A, B, and C solutions at a volume ratio of 1:1.6:0.2 to obtain the monohydric phenol derivative urine detection reagent.

[0023] Reagent stability: It can be stored for 2 years at room temperature (4°C-40°C), and has good stability.

[0024] Make a corresponding color comparison card, add five times the volume of urine to the reagent, observe the color of the complex, and compare it with the standard color comp...

Embodiment 2

[0026] 1. take by weighing mercuric sulfate and be dissolved in the sulfuric acid aqueous solution of 20% mass concentration and obtain A solution, in the solution, mercuric sulfate content is 500g / l;

[0027] 2. Take a certain volume of chlorosulfonic acid and dissolve it in deionized water to obtain solution B. The content of chlorosulfonic acid in the solution is 50ml / l;

[0028] 3. Take a certain volume of sulfolane and dissolve it in deionized water to obtain solution C. The sulfolane content in the solution is 60 l / l;

[0029] 4. Mixing: Mix the prepared A, B, and C solutions at a volume ratio of 1:1.2:0.15 to obtain the monohydric phenol derivative urine detection reagent.

[0030] Reagent stability: It can be stored for 2 years at room temperature (4°C-40°C), and has good stability.

[0031] Make a corresponding color comparison card, add five times the volume of urine to the reagent, observe the color of the complex, and compare it with the standard color comparison ...

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Abstract

The invention relates to a preparation method of a reagent for detection of monohydroxyphenol derivatives by sulfonation reaction. The monohydroxyphenol derivatives include tyrosine, p-hydroxyphenylalanine, tryptophan and 5-hydroxyindoleacetic acid. The monohydroxyphenol derivative urine detection reagent is an aqueous solution composed of sulfuric acid, mercury sulfate, chlorosulfonic acid and sulfolane. The preparation method includes: preparation a solution A, preparation of a solution B, preparation of a solution C, and finally mixing of the three solutions in certain proportion. According to the technical scheme provided by the invention, the raw materials are cheap and easily available, the preparation process is simple, the reagent has good stability, and has the characteristics of accurate testing, high sensitivity and good specificity, etc., the reaction time is short during detection, the result is easily judged, and the reagent is especially suitable for large-scale population general survey, clinical auxiliary diagnosis of cancer and dynamic check of efficacy.

Description

technical field [0001] The invention relates to a preparation method of a reagent for detecting monohydric phenol derivatives, in particular to a reagent for detecting monohydric phenol derivatives in urine by using sulfuric acid sulfonation reaction and a preparation method thereof. The monohydric phenolic derivatives include tyrosine, p-hydroxyphenylalanine, tryptophan and 5-hydroxyindoleacetic acid. The monohydric phenol derivative urine detection reagent is an aqueous solution composed of sulfuric acid, mercury sulfate, chlorosulfonic acid and sulfolane. The configuration method includes: preparation of A solution, preparation of B solution, preparation of C solution, and finally mixing the three solutions according to a certain ratio. The technical solution provided by the invention has the advantages of low raw material price and easy acquisition, simple preparation process, high reagent stability, accurate test, high sensitivity and good specificity, short reaction tim...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 温云飞王成林黄冬琴姜文彬赵美青
Owner 青岛浩铂生物科技有限公司
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