A synergistic anti-tumor polyphenol composition and its application
A polyphenol composition and a synergistic technology, applied in the field of medicine, can solve the problems of organ damage, damage to normal cells, low immune function, etc., and achieve the effect of reducing the dose and reducing the occurrence of drug resistance of tumor cells
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Embodiment 1
[0024] A synergistic anti-tumor polyphenol composition is a composition of quercetin and alfalfa.
[0025] The enhancing effect of quercetin combined with alfalfa in inhibiting the proliferation of human breast cancer MCF-7 cells (purchased from ATCC, USA) in vitro:
[0026] Human breast cancer MCF-7 cells of passage 10-30 were selected. Remove the cell culture medium (DMEM, purchased from Hyclone Company, USA), and wash with cold PBS. Then, the adherent cells were digested with trypsin (purchased from Gibco, USA). The digestion time was 2 min, and 4 mL of wash medium (purchased from Hyclone, USA) was added to terminate the digestion. Use a pipette to transfer the culture solution to a centrifuge tube, and centrifuge at 760 rpm for 4-5 minutes at a temperature of 4°C. cells in 2.5 x 10 4 The density of cells / well was spread in a 96-well plate, 100 μL per well. at 5% CO 2After culturing at 37°C for 6 hours in the incubator, 100 μL of a composition solution of quercetin an...
Embodiment 2
[0033] A synergistic anti-tumor polyphenol composition is a composition of luteolin and alfalfa.
[0034] Enhancement effect of luteolin combined with alfalfa in inhibiting the proliferation of human breast cancer MCF-7 cells in vitro:
[0035] Human breast cancer MCF-7 cells (purchased from ATCC, USA) of passage 10-30 were selected. Remove the cell culture medium (DMEM, purchased from Hyclone Company, USA), and wash with cold PBS. Then, the adherent cells were digested with trypsin (purchased from Gibco, USA). The digestion time was 2 min, and 4 mL of wash medium (purchased from Hyclone, USA) was added to terminate the digestion. Use a pipette to transfer the culture solution to a centrifuge tube, and centrifuge at 760 rpm for 4-5 minutes at a temperature of 4°C. cells in 2.5 x 10 4 The density of cells / well was spread in a 96-well plate, 100 μL per well. at 5% CO 2 After culturing for 6 hours at 37°C in the incubator, add 100 μL of the composition solution of luteolin ...
Embodiment 3
[0042] A synergistic anti-tumor polyphenol composition is a composition of p-coumaric acid and luteolin.
[0043] Synergistic effect of p-coumaric acid combined with luteolin in inhibiting the proliferation of human breast cancer MCF-7 cells in vitro:
[0044] Human breast cancer MCF-7 cells (purchased from ATCC, USA) of passage 10-30 were selected. Remove the cell culture medium (DMEM, purchased from Hyclone Company, USA), and wash with cold PBS. Then, the adherent cells were digested with trypsin (purchased from Gibco, USA). The digestion time was 2 min, and 4 mL of wash medium (purchased from Hyclone, USA) was added to terminate the digestion. Use a pipette to transfer the culture solution to a centrifuge tube, and centrifuge at 760 rpm for 4-5 minutes at a temperature of 4°C. cells in 2.5 x 10 4 The density of cells / well was spread in a 96-well plate, 100 μL per well. at 5% CO 2 After culturing for 6 hours at 37°C in the incubator, add 100 μL of a composition solutio...
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