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Tremella fuciformis strain and application thereof

A technology of white fungus and strains, applied in fungi, microorganisms, biochemical equipment and methods, etc., can solve the problems of low molecular weight and low yield, and achieve the effects of large molecular weight, high yield and high content

Active Publication Date: 2017-12-08
BLOOMAGE BIOTECHNOLOGY CORP LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of low molecular weight and low yield in the production of Tremella polysaccharide by spore fermentation, the present invention provides a Tremella spore strain HX5-1 screened from nature and the application of the strain in the production of Tremella polysaccharide by fermentation

Method used

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  • Tremella fuciformis strain and application thereof
  • Tremella fuciformis strain and application thereof
  • Tremella fuciformis strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Isolation, purification and identification of bacterial strains

[0039] Selection of seed ears: Collect and select wild white fungus with large flowers, white color, thick flesh, normal spreading, no bacteria and pest infection, and 80-90% maturity as seed ears from all over the country. After the seed ear is sterilized with sterile water, the bacteria on the spores are removed, and the surface of the ear piece is dried with sterile gauze or absorbent paper.

[0040] Ejection of spores: take a small piece of selected seed ear and make it hang in the middle of the triangular flask without touching the wall of the bottle or the culture medium below the bottle. The seed ear is 4-5 cm away from the surface of the culture medium. Place the triangular flask in an incubator and incubate at 30°C for 2 days. When foggy spores and small colonies grow on the surface of the medium, take out the seed ears, plug them with cotton plugs, and continue the cultivation. After ...

Embodiment 2

[0045] Example 2 Selection of bacterial strain fermentation liquid medium

[0046] 2.1 Types of nitrogen sources

[0047] Inoculate the spores of Tremella fungus HX5-1 into the basal medium (tryptone 10 g / L, glucose 20 g / L, potassium dihydrogen phosphate 3 g / L, magnesium sulfate 1.5 g / L and V B1 15mg / L; pH value is 6.0), fermented in a shaking flask at 200 r / min at 30°C for 3 days to obtain a working seed solution;

[0048] Put the working seed solution into a 300 mL Erlenmeyer flask with a 3% inoculum volume. The medium formula is shown in the table below. The fermentation was completed after 144 h at 30 °C and the rotation speed was 200 r / min, and the viscosity was measured. and polysaccharide content.

[0049] Table 2 The amount of polysaccharide produced by medium with different nitrogen sources

[0050]

[0051] Note: (1) soybean peptone 10 g / L, glucose 20 g / L, potassium dihydrogen phosphate 3 g / L, magnesium sulfate 1.5 g / L and V B1 15mg / L;

[0052] (2) Yeast pep...

Embodiment 3

[0061] Example 3 Production of High Molecular Weight Tremella Polysaccharide

[0062] (1) Preparation of seed liquid: Inoculate Tremella fungus HX5-1 into liquid medium (barley peptone 5 g / L, yeast peptone 5 g / L, glucose 20 g / L, potassium dihydrogen phosphate 3 g / L, magnesium sulfate 1.5 g / L and V B1 15mg / L; pH value is 6.0), 200r / min shaking flask fermentation at 30℃ for 3 days to obtain seed liquid;

[0063] (2) Three-stage aerated fermentation: put the seed liquid into a 10 L fermenter with 3% inoculation amount, the medium formula is as in step (1), culture with aeration and stirring at 30°C, the rotation speed is 200 r / min, and the aeration volume is: Initially 10 L / min, 15 L / min after 24 h, 20 L / min after 48 h until the end of the fermentation, the pH value was adjusted to 6.0-7.0 with 40% NaOH during the fermentation process, fed intermittently by feeding, and fermented to no Finish the fermentation when there is residual sugar, and obtain a fermented liquid (viscos...

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Abstract

The invention discloses a tremella fuciformis spore strain HX5-1 capable of producing tremella polysaccharide in high yield. The tremella fuciformis spore strain HX5-1 is preserved in CCTCC (China Center for Type Culture Collection), and the preservation number is CCTCC M 2017271. The invention also provides a method for producing polymer tremella polysaccharide by utilizing the tremella fuciformis spore strain HX5-1 trough fermentation. A tremella polysaccharide product is obtained by adopting the steps of seed solution preparation, three-stage aerated fermentation, alcohol precipitation, decoloration, deproteinization, refining, drying and the like. According to the method provided by the invention, through the three-stage aerated fermentation, the yield of the tremella polysaccharide can be up to 18 g / L; the tremella polysaccharide product is obtained through purifying and refining, the total saccharide content is greater than 80 percent, the molecular weight is greater than 1500 KDa, the protein content is smaller than 0.5 percent, the polysaccharide content is high, the molecular weight is high, and a wide application prospect in tremella fuciformis industrial production is obtained.

Description

technical field [0001] The invention relates to a tremella spore and the application of the strain in the production of high molecular weight tremella polysaccharides, belonging to the field of microbial fermentation engineering. Background technique [0002] Tremella polysaccharide is a kind of acid heteropolysaccharide, from Tremella ( Tremella fuciformis Berk) fruiting bodies, tremella spores or tremella steamed stuffed bun fermentation liquid, its main chain structure is mannan linked by α-(1→3) glycosidic bonds, and the branch chain is composed of glucuronic acid and xylose. [0003] Tremella polysaccharide has the effect of improving the immune function of the body and increasing white blood cells: it can significantly improve the phagocytosis of reticuloendothelial cells in experimental animals, promote non-specific immunity, and increase the level of immunoglobulin and serum total complement. Tremella polysaccharide can also increase the activity of SOD in the brai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N15/04C12P19/04C12R1/645
CPCC12N15/04C12P19/04C12N1/145C12R2001/645
Inventor 张天萌郭文逸孙劭靖郭学平
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
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