High performance liquid chromatography detection method of Cangrelor intermediate adenosine-2-thione
A high-performance liquid chromatography and detection method technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unseen compound detection methods and other reports, and achieve accurate and reliable detection results, easy operation, and high plate number Effect
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Embodiment 1
[0041] This embodiment provides a high-performance liquid chromatography detection method for Cangrelor intermediate adenosine-2-thione, comprising the following steps:
[0042] Preparation of standard solution: Accurately weigh 0.100 g of the product adenosine-2-thione, dissolve it in 0.1 mol / L NaOH solution, and set the volume to 100 mL. Filter with a 0.45 μm microporous membrane to obtain a standard solution, and take 5 μl for injection;
[0043] The standard solution is detected by reversed-phase high-performance liquid chromatography, and the detection conditions are:
[0044] The stationary phase of the chromatographic column: silica gel with a non-polar octadecyl functional group (ODS) bonded to the surface as a filler;
[0045] Mobile phase: acetonitrile and 0.3% acetic acid aqueous solution (adjust the pH to 5.8 with triethylamine) (5.5:94.5(V:V));
[0046] Flow rate: 0.5ml / min;
[0047] Column temperature: 25°C;
[0048] Detection wavelength: 254nm;
[0049] Acc...
Embodiment 2
[0056] This embodiment provides the existing high performance liquid chromatography detection method of Cangrelor intermediate adenosine-2-thione, comprising the following steps:
[0057] Preparation of standard solution: Accurately weigh 0.010 g of the product adenosine-2-thione, dissolve it in pure water, and set the volume to 100 mL. Filter with a 0.45 μm microporous membrane to obtain a standard solution, and take 5 μl for injection;
[0058] The standard solution is detected by reversed-phase high-performance liquid chromatography, and the detection conditions are:
[0059]The stationary phase of the chromatographic column: silica gel with a non-polar octadecyl functional group (ODS) bonded to the surface as a filler;
[0060] Mobile phase: acetonitrile and phosphoric acid aqueous solution (pH=3) (5:95-30:70 (V:V) gradient elution);
[0061] Flow rate: 1ml / min;
[0062] Column temperature: 25°C;
[0063] Detection wavelength: 260nm;
[0064] According to the above de...
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