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Application of mir-339-5p in inhibiting prostate cancer bone metastasis and TGF-β signaling pathway

A technology for prostate cancer and bone metastasis, applied in the field of molecular biology, can solve the problems that affect the treatment effect of tumors and have no research reports

Active Publication Date: 2019-11-05
THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemoradiotherapy drugs not only act on the tumor, but can also act on the healthy tissues around the tumor. Therefore, while killing the tumor, they also bring great side effects to the body, which ultimately affects the therapeutic effect on the tumor.
At present, there is no research report on the relationship between miR-339-5p and prostate cancer bone metastasis and TGF-β signaling pathway

Method used

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  • Application of mir-339-5p in inhibiting prostate cancer bone metastasis and TGF-β signaling pathway
  • Application of mir-339-5p in inhibiting prostate cancer bone metastasis and TGF-β signaling pathway
  • Application of mir-339-5p in inhibiting prostate cancer bone metastasis and TGF-β signaling pathway

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Experimental program
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Effect test

experiment example 1

[0029] Experimental example 1. miR-339-5p is highly expressed in prostate cancer tissues, but it is low expressed in bone metastatic prostate cancer tissues

[0030] The inventors used the data in the TCGA database to conduct bioinformatics analysis and found that miR-339-5p was highly expressed in prostate cancer tissues, such as figure 1 As shown, compared with 52 cases of paracancerous tissues, the expression of miR-339-5p in 498 cases of prostate cancer tissues was significantly increased ( figure 1 A), Consistently, miR-339-5p was highly expressed in 52 paired prostate cancer tissues compared with paired paracancerous tissues ( figure 1 B). However, unexpectedly, the inventor found that miR-339-5p was lowly expressed in prostate cancer tissue with bone metastases. The research method is as follows:

[0031] RNA extraction, reverse transcription and real-time quantitative RT-PCR detection of miR-339-5p: using TRIzol lysate (LifeTechnologies) to extract total RNA from all...

experiment example 2

[0034] Experimental example 2. Molecular mechanism of miR-339-5p inhibiting bone metastasis of prostate cancer

[0035] experimental method:

[0036] (1) RNA extraction, reverse transcription and real-time quantitative PCR

[0037] The total RNA of all tissues and cells was extracted using TRIzol lysate (Life Technologies Company) instructions, mRNA and miRNA were reverse-transcribed according to the Revert Aid First Strand cDNA Synthesis Kit (Thermo Fisher Company) instructions, and the 2-ddCt relative quantification method was used for fluorescence quantification For detection, the relative expression levels of miRNA and mRNA were calculated using U6 and GAPDH as internal references, respectively.

[0038] (2) Plasmid construction, siRNA Using the human genome as a template, amplify the human miR-339-5p (miRBase number MIMAT0000764) gene, and clone it into the pMSCV-puro retroviral vector (Clontech Company) to obtain miR-339-5p The expression plasmid is used to up-regulate...

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Abstract

The invention discloses an application of miR-339-5p in retraining prostate cancer with osseous metastasis and in a TGF-beta signal channel. The invention researches and finds that the miR-339-5p has low expression in prostate cancer with osseous metastasis and a further research proves that the miR-339-5p is capable of restraining the activity of the TGF-beta signal channel through SMAD2, SMAD3 and TGFBRI in a targeting TGF-beta signal channel so as to restrain the migration and invasion of the cells of prostate cancer with osseous metastasis, so that the miR-339-5p is expected to be used for preparing a diagnostic reagent for prostate cancer with osseous metastasis, a drug for restraining osseous metastasis, a TGF-beta / smad inhibitor, an SMAD2 inhibitor, an SMAD3 inhibitor and a TGFBRI inhibitor.

Description

technical field [0001] The invention belongs to the field of molecular biology, and more specifically relates to the application of miR-339-5p in inhibiting prostate cancer bone metastasis and TGF-β signaling pathway. Background technique [0002] Prostate cancer (PCa) is one of the most common malignant tumors of the male genitourinary system. Cancer metastasis will greatly increase the mortality of prostate cancer patients. Bone is the second most common site of cancer metastasis. In my country, more than 80% of prostate cancer patients have had bone metastasis at the time of diagnosis, and bone metastasis is the leading cause of death in prostate cancer patients. Important reason, therefore, the early diagnosis of bone metastases is the key to clinical diagnosis and prognosis. In addition, for patients with advanced prostate cancer, the effect of surgical treatment is poor, and most of them adopt drug treatment. Depending on the condition, chemical drug therapy, external...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888A61K48/00A61K31/7088A61P35/04
CPCA61K31/7088C12Q1/6886C12Q2600/158C12Q2600/178
Inventor 黄帅唐欲博瓦庆德彭新生郭远清
Owner THE SECOND AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
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